Monitoring the Levels of Cellular NF-κB Activation States

The NF-κB signaling system plays an important regulatory role in the control of many biological processes. The activities of NF-κB signaling networks and the expression of their target genes are frequently elevated in pathophysiological situations including inflammation, infection, and cancer. In th...

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Autores principales:	Johanna Meier-Soelch, Christin Mayr-Buro, Jana Juli, Lisa Leib, Uwe Linne, Jan Dreute, Argyris Papantonis, M. Lienhard Schmitz, Michael Kracht
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	NF-κB signaling kinase transcription factor methods single cell assays Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/426544c935b74634922da09fe4a6efd5
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id	oai:doaj.org-article:426544c935b74634922da09fe4a6efd5
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spelling	oai:doaj.org-article:426544c935b74634922da09fe4a6efd52021-11-11T15:28:54ZMonitoring the Levels of Cellular NF-κB Activation States10.3390/cancers132153512072-6694https://doaj.org/article/426544c935b74634922da09fe4a6efd52021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/21/5351https://doaj.org/toc/2072-6694The NF-κB signaling system plays an important regulatory role in the control of many biological processes. The activities of NF-κB signaling networks and the expression of their target genes are frequently elevated in pathophysiological situations including inflammation, infection, and cancer. In these conditions, the outcome of NF-κB activity can vary according to (i) differential activation states, (ii) the pattern of genomic recruitment of the NF-κB subunits, and (iii) cellular heterogeneity. Additionally, the cytosolic NF-κB activation steps leading to the liberation of DNA-binding dimers need to be distinguished from the less understood nuclear pathways that are ultimately responsible for NF-κB target gene specificity. This raises the need to more precisely determine the NF-κB activation status not only for the purpose of basic research, but also in (future) clinical applications. Here we review a compendium of different methods that have been developed to assess the NF-κB activation status in vitro and in vivo. We also discuss recent advances that allow the assessment of several NF-κB features simultaneously at the single cell level.Johanna Meier-SoelchChristin Mayr-BuroJana JuliLisa LeibUwe LinneJan DreuteArgyris PapantonisM. Lienhard SchmitzMichael KrachtMDPI AGarticleNF-κBsignalingkinasetranscription factormethodssingle cell assaysNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5351, p 5351 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	NF-κB signaling kinase transcription factor methods single cell assays Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	NF-κB signaling kinase transcription factor methods single cell assays Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Johanna Meier-Soelch Christin Mayr-Buro Jana Juli Lisa Leib Uwe Linne Jan Dreute Argyris Papantonis M. Lienhard Schmitz Michael Kracht Monitoring the Levels of Cellular NF-κB Activation States
description	The NF-κB signaling system plays an important regulatory role in the control of many biological processes. The activities of NF-κB signaling networks and the expression of their target genes are frequently elevated in pathophysiological situations including inflammation, infection, and cancer. In these conditions, the outcome of NF-κB activity can vary according to (i) differential activation states, (ii) the pattern of genomic recruitment of the NF-κB subunits, and (iii) cellular heterogeneity. Additionally, the cytosolic NF-κB activation steps leading to the liberation of DNA-binding dimers need to be distinguished from the less understood nuclear pathways that are ultimately responsible for NF-κB target gene specificity. This raises the need to more precisely determine the NF-κB activation status not only for the purpose of basic research, but also in (future) clinical applications. Here we review a compendium of different methods that have been developed to assess the NF-κB activation status in vitro and in vivo. We also discuss recent advances that allow the assessment of several NF-κB features simultaneously at the single cell level.
format	article
author	Johanna Meier-Soelch Christin Mayr-Buro Jana Juli Lisa Leib Uwe Linne Jan Dreute Argyris Papantonis M. Lienhard Schmitz Michael Kracht
author_facet	Johanna Meier-Soelch Christin Mayr-Buro Jana Juli Lisa Leib Uwe Linne Jan Dreute Argyris Papantonis M. Lienhard Schmitz Michael Kracht
author_sort	Johanna Meier-Soelch
title	Monitoring the Levels of Cellular NF-κB Activation States
title_short	Monitoring the Levels of Cellular NF-κB Activation States
title_full	Monitoring the Levels of Cellular NF-κB Activation States
title_fullStr	Monitoring the Levels of Cellular NF-κB Activation States
title_full_unstemmed	Monitoring the Levels of Cellular NF-κB Activation States
title_sort	monitoring the levels of cellular nf-κb activation states
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/426544c935b74634922da09fe4a6efd5
work_keys_str_mv	AT johannameiersoelch monitoringthelevelsofcellularnfkbactivationstates AT christinmayrburo monitoringthelevelsofcellularnfkbactivationstates AT janajuli monitoringthelevelsofcellularnfkbactivationstates AT lisaleib monitoringthelevelsofcellularnfkbactivationstates AT uwelinne monitoringthelevelsofcellularnfkbactivationstates AT jandreute monitoringthelevelsofcellularnfkbactivationstates AT argyrispapantonis monitoringthelevelsofcellularnfkbactivationstates AT mlienhardschmitz monitoringthelevelsofcellularnfkbactivationstates AT michaelkracht monitoringthelevelsofcellularnfkbactivationstates
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Monitoring the Levels of Cellular NF-κB Activation States

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