The rescue of miR-148a expression in pancreatic cancer: an inappropriate therapeutic tool.

MicroRNAs are small non-coding RNAs that physiologically modulate proteins expression, and regulate numerous cellular mechanisms. Alteration of microRNA expression has been described in cancer and is associated to tumor initiation and progression. The microRNA 148a (miR-148a) is frequently down-regu...

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Autores principales:	Yannick Delpu, Hubert Lulka, Flavie Sicard, Nathalie Saint-Laurent, Frédéric Lopez, Naïma Hanoun, Louis Buscail, Pierre Cordelier, Jérôme Torrisani
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/42695a09c3c249bf9e5d1226d06f4ee3
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spelling	oai:doaj.org-article:42695a09c3c249bf9e5d1226d06f4ee32021-11-18T07:59:08ZThe rescue of miR-148a expression in pancreatic cancer: an inappropriate therapeutic tool.1932-620310.1371/journal.pone.0055513https://doaj.org/article/42695a09c3c249bf9e5d1226d06f4ee32013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23383211/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203MicroRNAs are small non-coding RNAs that physiologically modulate proteins expression, and regulate numerous cellular mechanisms. Alteration of microRNA expression has been described in cancer and is associated to tumor initiation and progression. The microRNA 148a (miR-148a) is frequently down-regulated in cancer. We previously demonstrated that its down-regulation by DNA hypermethylation is an early event in pancreatic ductal adenocarcinoma (PDAC) carcinogenesis, suggesting a tumor suppressive function. Here, we investigate the potential role of miR-148a over-expression in PDAC as a therapeutic tool. We first report the consequences of miR-148a over-expression in PDAC cell lines. We demonstrate that miR-148a over-expression has no dramatic effect on cell proliferation and cell chemo-sensitivity in four well described PDAC cell lines. We also investigate the modulation of protein expression by a global proteomic approach (2D-DIGE). We show that despite its massive over-expression, miR-148a weakly modulates protein expression, thus preventing the identification of protein targets in PDAC cell lines. More importantly, in vivo data demonstrate that modulating miR-148a expression either in the epithelia tumor cells and/or in the tumor microenvironment does not impede tumor growth. Taken together, we demonstrate herein that miR-148a does not impact PDAC proliferation both in vitro and in vivo thus suggesting a weak potential as a therapeutic tool.Yannick DelpuHubert LulkaFlavie SicardNathalie Saint-LaurentFrédéric LopezNaïma HanounLouis BuscailPierre CordelierJérôme TorrisaniPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e55513 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yannick Delpu Hubert Lulka Flavie Sicard Nathalie Saint-Laurent Frédéric Lopez Naïma Hanoun Louis Buscail Pierre Cordelier Jérôme Torrisani The rescue of miR-148a expression in pancreatic cancer: an inappropriate therapeutic tool.
description	MicroRNAs are small non-coding RNAs that physiologically modulate proteins expression, and regulate numerous cellular mechanisms. Alteration of microRNA expression has been described in cancer and is associated to tumor initiation and progression. The microRNA 148a (miR-148a) is frequently down-regulated in cancer. We previously demonstrated that its down-regulation by DNA hypermethylation is an early event in pancreatic ductal adenocarcinoma (PDAC) carcinogenesis, suggesting a tumor suppressive function. Here, we investigate the potential role of miR-148a over-expression in PDAC as a therapeutic tool. We first report the consequences of miR-148a over-expression in PDAC cell lines. We demonstrate that miR-148a over-expression has no dramatic effect on cell proliferation and cell chemo-sensitivity in four well described PDAC cell lines. We also investigate the modulation of protein expression by a global proteomic approach (2D-DIGE). We show that despite its massive over-expression, miR-148a weakly modulates protein expression, thus preventing the identification of protein targets in PDAC cell lines. More importantly, in vivo data demonstrate that modulating miR-148a expression either in the epithelia tumor cells and/or in the tumor microenvironment does not impede tumor growth. Taken together, we demonstrate herein that miR-148a does not impact PDAC proliferation both in vitro and in vivo thus suggesting a weak potential as a therapeutic tool.
format	article
author	Yannick Delpu Hubert Lulka Flavie Sicard Nathalie Saint-Laurent Frédéric Lopez Naïma Hanoun Louis Buscail Pierre Cordelier Jérôme Torrisani
author_facet	Yannick Delpu Hubert Lulka Flavie Sicard Nathalie Saint-Laurent Frédéric Lopez Naïma Hanoun Louis Buscail Pierre Cordelier Jérôme Torrisani
author_sort	Yannick Delpu
title	The rescue of miR-148a expression in pancreatic cancer: an inappropriate therapeutic tool.
title_short	The rescue of miR-148a expression in pancreatic cancer: an inappropriate therapeutic tool.
title_full	The rescue of miR-148a expression in pancreatic cancer: an inappropriate therapeutic tool.
title_fullStr	The rescue of miR-148a expression in pancreatic cancer: an inappropriate therapeutic tool.
title_full_unstemmed	The rescue of miR-148a expression in pancreatic cancer: an inappropriate therapeutic tool.
title_sort	rescue of mir-148a expression in pancreatic cancer: an inappropriate therapeutic tool.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/42695a09c3c249bf9e5d1226d06f4ee3
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The rescue of miR-148a expression in pancreatic cancer: an inappropriate therapeutic tool.

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