Distribution of Antibiotic Resistance Genes in Enterococcus spp. Isolated from Mastitis Bovine Milk

In this study, determination of enterococcus species that were isolated from mastitic milk samples, investigation of their susceptibilities to antibiotics and identification of the existence of resistance genes in resistant strains were conducted. The specimens consist of 600 mastitic milk samples t...

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Autores principales:	Erbas Goksel, Parin Ugur, Turkyilmaz Suheyla, Ucan Nese, Ozturk Mehmet, Kaya Osman
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2016
Materias:	antibiotic resistantance genes entrococcus mastitis pcr vana Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/426bbc82d5f8435cb2d714fe1f8fc8b3
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spelling	oai:doaj.org-article:426bbc82d5f8435cb2d714fe1f8fc8b32021-11-17T21:27:51ZDistribution of Antibiotic Resistance Genes in Enterococcus spp. Isolated from Mastitis Bovine Milk1820-744810.1515/acve-2016-0029https://doaj.org/article/426bbc82d5f8435cb2d714fe1f8fc8b32016-09-01T00:00:00Zhttps://doi.org/10.1515/acve-2016-0029https://doaj.org/toc/1820-7448In this study, determination of enterococcus species that were isolated from mastitic milk samples, investigation of their susceptibilities to antibiotics and identification of the existence of resistance genes in resistant strains were conducted. The specimens consist of 600 mastitic milk samples that were collected from 242 cows. Isolation of enterococcus was carried out in selective media and 94 (15.6%) Enterococcus spp. were isolated. A total of 94 species of Enterococci were identified using both sequencing and polymerase chain reaction (PCR). Enterococcus spp. isolates belong to 5 different species (E. faecalis, E. faecium, E. durans, E. hirae, E. mundtii) in sequence analysis and 4 different species (E. faecalis, E. faecium, E. durans, E. hirae) were identify by PCR method with specific primers. Analyzing 94 enterococcus strains by antibiotic sensitiveness test a high rate of resistance to tetracycline in 77 (81.9%) isolates was shown. The tet resistance genes were identified as follows: 54 were tetM positive, 23 were tetK positive and 17 were positive on tetM and tetK. Resistance to erythromycin was established in 27 (28.7%) isolates (25 ermB) while the chloramphenicol resistance gene was found in 10 (10.7%) of isolates and the cat gene was identified in nine samples and one isolate was resistant to vancomycin (1.06%) with the VanA gene confirmed. In conclusion, it was shown that E. faecalis has the biggest role in enterococcus originated mastitis and these strains were found to be mostly resistant to tetracycline. One vancomycine resistant isolate that had the VanA gene was also determined.Erbas GokselParin UgurTurkyilmaz SuheylaUcan NeseOzturk MehmetKaya OsmanSciendoarticleantibiotic resistantance genesentrococcusmastitispcrvanaVeterinary medicineSF600-1100ENActa Veterinaria, Vol 66, Iss 3, Pp 336-346 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibiotic resistantance genes entrococcus mastitis pcr vana Veterinary medicine SF600-1100
spellingShingle	antibiotic resistantance genes entrococcus mastitis pcr vana Veterinary medicine SF600-1100 Erbas Goksel Parin Ugur Turkyilmaz Suheyla Ucan Nese Ozturk Mehmet Kaya Osman Distribution of Antibiotic Resistance Genes in Enterococcus spp. Isolated from Mastitis Bovine Milk
description	In this study, determination of enterococcus species that were isolated from mastitic milk samples, investigation of their susceptibilities to antibiotics and identification of the existence of resistance genes in resistant strains were conducted. The specimens consist of 600 mastitic milk samples that were collected from 242 cows. Isolation of enterococcus was carried out in selective media and 94 (15.6%) Enterococcus spp. were isolated. A total of 94 species of Enterococci were identified using both sequencing and polymerase chain reaction (PCR). Enterococcus spp. isolates belong to 5 different species (E. faecalis, E. faecium, E. durans, E. hirae, E. mundtii) in sequence analysis and 4 different species (E. faecalis, E. faecium, E. durans, E. hirae) were identify by PCR method with specific primers. Analyzing 94 enterococcus strains by antibiotic sensitiveness test a high rate of resistance to tetracycline in 77 (81.9%) isolates was shown. The tet resistance genes were identified as follows: 54 were tetM positive, 23 were tetK positive and 17 were positive on tetM and tetK. Resistance to erythromycin was established in 27 (28.7%) isolates (25 ermB) while the chloramphenicol resistance gene was found in 10 (10.7%) of isolates and the cat gene was identified in nine samples and one isolate was resistant to vancomycin (1.06%) with the VanA gene confirmed. In conclusion, it was shown that E. faecalis has the biggest role in enterococcus originated mastitis and these strains were found to be mostly resistant to tetracycline. One vancomycine resistant isolate that had the VanA gene was also determined.
format	article
author	Erbas Goksel Parin Ugur Turkyilmaz Suheyla Ucan Nese Ozturk Mehmet Kaya Osman
author_facet	Erbas Goksel Parin Ugur Turkyilmaz Suheyla Ucan Nese Ozturk Mehmet Kaya Osman
author_sort	Erbas Goksel
title	Distribution of Antibiotic Resistance Genes in Enterococcus spp. Isolated from Mastitis Bovine Milk
title_short	Distribution of Antibiotic Resistance Genes in Enterococcus spp. Isolated from Mastitis Bovine Milk
title_full	Distribution of Antibiotic Resistance Genes in Enterococcus spp. Isolated from Mastitis Bovine Milk
title_fullStr	Distribution of Antibiotic Resistance Genes in Enterococcus spp. Isolated from Mastitis Bovine Milk
title_full_unstemmed	Distribution of Antibiotic Resistance Genes in Enterococcus spp. Isolated from Mastitis Bovine Milk
title_sort	distribution of antibiotic resistance genes in enterococcus spp. isolated from mastitis bovine milk
publisher	Sciendo
publishDate	2016
url	https://doaj.org/article/426bbc82d5f8435cb2d714fe1f8fc8b3
work_keys_str_mv	AT erbasgoksel distributionofantibioticresistancegenesinenterococcussppisolatedfrommastitisbovinemilk AT parinugur distributionofantibioticresistancegenesinenterococcussppisolatedfrommastitisbovinemilk AT turkyilmazsuheyla distributionofantibioticresistancegenesinenterococcussppisolatedfrommastitisbovinemilk AT ucannese distributionofantibioticresistancegenesinenterococcussppisolatedfrommastitisbovinemilk AT ozturkmehmet distributionofantibioticresistancegenesinenterococcussppisolatedfrommastitisbovinemilk AT kayaosman distributionofantibioticresistancegenesinenterococcussppisolatedfrommastitisbovinemilk
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Distribution of Antibiotic Resistance Genes in Enterococcus spp. Isolated from Mastitis Bovine Milk

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