Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research

Helena Debiazi Zomer,1,2 Kelly CS Roballo,2 Thais Borges Lessa,2 Fabiana Fernandes Bressan,2 Natália Nardeli Gonçalves,2 Flávio Vieira Meirelles,2,3 Andrea Gonçalves Trentin,1 Carlos Eduardo Ambrósio2,3 1Department of Cell Biology, Embryology an...

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Autores principales:	Zomer HD, Roballo KCS, Lessa TB, Bressan FF, Gonçalves NN, Meirelles FV, Trentin AG, Ambrósio CE
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Characterization comparison MSC iPS immunophenotype proliferation Cytology QH573-671
Acceso en línea:	https://doaj.org/article/426be1746e7a4ff681e6d93e94bd4fa1
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spelling	oai:doaj.org-article:426be1746e7a4ff681e6d93e94bd4fa12021-12-02T08:54:24ZDistinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research1178-6957https://doaj.org/article/426be1746e7a4ff681e6d93e94bd4fa12018-10-01T00:00:00Zhttps://www.dovepress.com/distinct-features-of-rabbit-and-human-adipose-derived-mesenchymal-stem-peer-reviewed-article-SCCAAhttps://doaj.org/toc/1178-6957Helena Debiazi Zomer,1,2 Kelly CS Roballo,2 Thais Borges Lessa,2 Fabiana Fernandes Bressan,2 Natália Nardeli Gonçalves,2 Flávio Vieira Meirelles,2,3 Andrea Gonçalves Trentin,1 Carlos Eduardo Ambrósio2,3 1Department of Cell Biology, Embryology and Genetic, Faculty of Biological Sciences, Santa Catarina Federal University (UFSC), Florianópolis, Brazil; 2Department of Surgery, Sector Anatomy, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil; 3Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil Introduction: Owing to their similarity with humans, rabbits are useful for multiple applications in biotechnology and translational research from basic to preclinical studies. In this sense, mesenchymal stem cells (MSCs) are known for their therapeutic potential and promising future in regenerative medicine. As many studies have been using rabbit adipose-derived MSCs (ASCs) as a model of human ASCs (hASCs), it is fundamental to compare their characteristics and understand how distinct features could affect the translation to human medicine.Objective: The aim of this study was to comparatively characterize rabbit ASCs (rASCs) and hASCs to further uses in biotechnology and translational studies.Materials and methods: rASCs and hASCs were isolated and characterized by their immunophenotype, differentiation potential, proliferative profile, and nuclear stability in vitro.Results and discussion: Both ASCs presented differentiation potential to osteocytes, chondrocytes, and adipocytes and shared similar immunophenotype expression to CD105+, CD34–, and CD45–, but rabbit cells expressed significantly lower CD73 and CD90 than human cells. In addition, rASCs presented greater clonogenic potential and proliferation rate than hASCs but no difference in nuclear alterations.Conclusion: The distinct features of rASCs and hASCs can positively or negatively affect their use for different applications in biotechnology (such as cell reprogramming) and translational studies (such as cell transplantation, tissue engineering, and pharmacokinetics). Nevertheless, the particularities between rabbit and human MSCs should not prevent rabbit use in preclinical models, but care should be taken to interpret results and properly translate animal findings to medicine.Keywords: characterization, comparison, MSC, iPS, immunophenotype, proliferation Zomer HDRoballo KCSLessa TBBressan FFGonçalves NNMeirelles FVTrentin AGAmbrósio CEDove Medical PressarticleCharacterizationcomparisonMSCiPSimmunophenotypeproliferationCytologyQH573-671ENStem Cells and Cloning: Advances and Applications, Vol Volume 11, Pp 43-54 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Characterization comparison MSC iPS immunophenotype proliferation Cytology QH573-671
spellingShingle	Characterization comparison MSC iPS immunophenotype proliferation Cytology QH573-671 Zomer HD Roballo KCS Lessa TB Bressan FF Gonçalves NN Meirelles FV Trentin AG Ambrósio CE Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research
description	Helena Debiazi Zomer,1,2 Kelly CS Roballo,2 Thais Borges Lessa,2 Fabiana Fernandes Bressan,2 Natália Nardeli Gonçalves,2 Flávio Vieira Meirelles,2,3 Andrea Gonçalves Trentin,1 Carlos Eduardo Ambrósio2,3 1Department of Cell Biology, Embryology and Genetic, Faculty of Biological Sciences, Santa Catarina Federal University (UFSC), Florianópolis, Brazil; 2Department of Surgery, Sector Anatomy, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil; 3Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil Introduction: Owing to their similarity with humans, rabbits are useful for multiple applications in biotechnology and translational research from basic to preclinical studies. In this sense, mesenchymal stem cells (MSCs) are known for their therapeutic potential and promising future in regenerative medicine. As many studies have been using rabbit adipose-derived MSCs (ASCs) as a model of human ASCs (hASCs), it is fundamental to compare their characteristics and understand how distinct features could affect the translation to human medicine.Objective: The aim of this study was to comparatively characterize rabbit ASCs (rASCs) and hASCs to further uses in biotechnology and translational studies.Materials and methods: rASCs and hASCs were isolated and characterized by their immunophenotype, differentiation potential, proliferative profile, and nuclear stability in vitro.Results and discussion: Both ASCs presented differentiation potential to osteocytes, chondrocytes, and adipocytes and shared similar immunophenotype expression to CD105+, CD34–, and CD45–, but rabbit cells expressed significantly lower CD73 and CD90 than human cells. In addition, rASCs presented greater clonogenic potential and proliferation rate than hASCs but no difference in nuclear alterations.Conclusion: The distinct features of rASCs and hASCs can positively or negatively affect their use for different applications in biotechnology (such as cell reprogramming) and translational studies (such as cell transplantation, tissue engineering, and pharmacokinetics). Nevertheless, the particularities between rabbit and human MSCs should not prevent rabbit use in preclinical models, but care should be taken to interpret results and properly translate animal findings to medicine.Keywords: characterization, comparison, MSC, iPS, immunophenotype, proliferation
format	article
author	Zomer HD Roballo KCS Lessa TB Bressan FF Gonçalves NN Meirelles FV Trentin AG Ambrósio CE
author_facet	Zomer HD Roballo KCS Lessa TB Bressan FF Gonçalves NN Meirelles FV Trentin AG Ambrósio CE
author_sort	Zomer HD
title	Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research
title_short	Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research
title_full	Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research
title_fullStr	Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research
title_full_unstemmed	Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research
title_sort	distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/426be1746e7a4ff681e6d93e94bd4fa1
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Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research

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