Cell type-specific response to high intracellular loading of polyacrylic acid-coated magnetic nanoparticles

Jasna Lojk,1 Vladimir B Bregar,1 Maruša Rajh,1 Katarina Miš,2 Mateja Erdani Kreft,3 Sergej Pirkmajer,2 Peter Veranič,3 Mojca Pavlin1 1Group for Nano and Biotechnological Applications, Faculty of Electrical Engineering, 2Institute of Pathophysiology, Faculty of Medicine, 3Inst...

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Autores principales: Lojk J, Bregar VB, Rajh M, Miš K, Kreft ME, Pirkmajer S, Veranič P, Pavlin M
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Lenguaje:EN
Publicado: Dove Medical Press 2015
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spelling oai:doaj.org-article:427cc83943d84b4eafff072c3b8482292021-12-02T02:53:31ZCell type-specific response to high intracellular loading of polyacrylic acid-coated magnetic nanoparticles1178-2013https://doaj.org/article/427cc83943d84b4eafff072c3b8482292015-02-01T00:00:00Zhttp://www.dovepress.com/cell-type-specific-response-to-high-intracellular-loading-of-polyacryl-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Jasna Lojk,1 Vladimir B Bregar,1 Maruša Rajh,1 Katarina Miš,2 Mateja Erdani Kreft,3 Sergej Pirkmajer,2 Peter Veranič,3 Mojca Pavlin1 1Group for Nano and Biotechnological Applications, Faculty of Electrical Engineering, 2Institute of Pathophysiology, Faculty of Medicine, 3Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia Abstract: Magnetic nanoparticles (NPs) are a special type of NP with a ferromagnetic, electron-dense core that enables several applications such as cell tracking, hyperthermia, and magnetic separation, as well as multimodality. So far, superparamagnetic iron oxide NPs (SPIONs) are the only clinically approved type of metal oxide NPs, but cobalt ferrite NPs have properties suitable for biomedical applications as well. In this study, we analyzed the cellular responses to magnetic cobalt ferrite NPs coated with polyacrylic acid (PAA) in three cell types: Chinese Hamster Ovary (CHO), mouse melanoma (B16) cell line, and primary human myoblasts (MYO). We compared the internalization pathway, intracellular trafficking, and intracellular fate of our NPs using fluorescence and transmission electron microscopy (TEM) as well as quantified NP uptake and analyzed uptake dynamics. We determined cell viability after 24 or 96 hours’ exposure to increasing concentrations of NPs, and quantified the generation of reactive oxygen species (ROS) upon 24 and 48 hours’ exposure. Our NPs have been shown to readily enter and accumulate in cells in high quantities using the same two endocytic pathways; mostly by macropinocytosis and partially by clathrin-mediated endocytosis. The cell types differed in their uptake rate, the dynamics of intracellular trafficking, and the uptake capacity, as well as in their response to higher concentrations of internalized NPs. The observed differences in cell responses stress the importance of evaluation of NP–cell interactions on several different cell types for better prediction of possible toxic effects on different cell and tissue types in vivo. Keywords: intracellular fate, transmission electron microscopy, uptake quantification, ROS, cell viabilityLojk JBregar VBRajh MMiš KKreft MEPirkmajer SVeranič PPavlin MDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 1449-1462 (2015)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Lojk J
Bregar VB
Rajh M
Miš K
Kreft ME
Pirkmajer S
Veranič P
Pavlin M
Cell type-specific response to high intracellular loading of polyacrylic acid-coated magnetic nanoparticles
description Jasna Lojk,1 Vladimir B Bregar,1 Maruša Rajh,1 Katarina Miš,2 Mateja Erdani Kreft,3 Sergej Pirkmajer,2 Peter Veranič,3 Mojca Pavlin1 1Group for Nano and Biotechnological Applications, Faculty of Electrical Engineering, 2Institute of Pathophysiology, Faculty of Medicine, 3Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia Abstract: Magnetic nanoparticles (NPs) are a special type of NP with a ferromagnetic, electron-dense core that enables several applications such as cell tracking, hyperthermia, and magnetic separation, as well as multimodality. So far, superparamagnetic iron oxide NPs (SPIONs) are the only clinically approved type of metal oxide NPs, but cobalt ferrite NPs have properties suitable for biomedical applications as well. In this study, we analyzed the cellular responses to magnetic cobalt ferrite NPs coated with polyacrylic acid (PAA) in three cell types: Chinese Hamster Ovary (CHO), mouse melanoma (B16) cell line, and primary human myoblasts (MYO). We compared the internalization pathway, intracellular trafficking, and intracellular fate of our NPs using fluorescence and transmission electron microscopy (TEM) as well as quantified NP uptake and analyzed uptake dynamics. We determined cell viability after 24 or 96 hours’ exposure to increasing concentrations of NPs, and quantified the generation of reactive oxygen species (ROS) upon 24 and 48 hours’ exposure. Our NPs have been shown to readily enter and accumulate in cells in high quantities using the same two endocytic pathways; mostly by macropinocytosis and partially by clathrin-mediated endocytosis. The cell types differed in their uptake rate, the dynamics of intracellular trafficking, and the uptake capacity, as well as in their response to higher concentrations of internalized NPs. The observed differences in cell responses stress the importance of evaluation of NP–cell interactions on several different cell types for better prediction of possible toxic effects on different cell and tissue types in vivo. Keywords: intracellular fate, transmission electron microscopy, uptake quantification, ROS, cell viability
format article
author Lojk J
Bregar VB
Rajh M
Miš K
Kreft ME
Pirkmajer S
Veranič P
Pavlin M
author_facet Lojk J
Bregar VB
Rajh M
Miš K
Kreft ME
Pirkmajer S
Veranič P
Pavlin M
author_sort Lojk J
title Cell type-specific response to high intracellular loading of polyacrylic acid-coated magnetic nanoparticles
title_short Cell type-specific response to high intracellular loading of polyacrylic acid-coated magnetic nanoparticles
title_full Cell type-specific response to high intracellular loading of polyacrylic acid-coated magnetic nanoparticles
title_fullStr Cell type-specific response to high intracellular loading of polyacrylic acid-coated magnetic nanoparticles
title_full_unstemmed Cell type-specific response to high intracellular loading of polyacrylic acid-coated magnetic nanoparticles
title_sort cell type-specific response to high intracellular loading of polyacrylic acid-coated magnetic nanoparticles
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/427cc83943d84b4eafff072c3b848229
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