Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs

Abstract The epidemic of obesity and diabetes has markedly spurred the research interest in adipocyte biology. Brown adipocytes are specialized for energy expenditure and of therapeutic interest for treatment of metabolic diseases, but how brown adipocytes are distinguished from white adipocytes at...

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Autores principales:	David W. Reid, Dan Xu, Peng Chen, Hongyuan Yang, Lei Sun
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/42814b23c8b94a7fb4c6fb81551195e8
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spelling	oai:doaj.org-article:42814b23c8b94a7fb4c6fb81551195e82021-12-02T11:41:12ZIntegrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs10.1038/s41598-017-06077-32045-2322https://doaj.org/article/42814b23c8b94a7fb4c6fb81551195e82017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06077-3https://doaj.org/toc/2045-2322Abstract The epidemic of obesity and diabetes has markedly spurred the research interest in adipocyte biology. Brown adipocytes are specialized for energy expenditure and of therapeutic interest for treatment of metabolic diseases, but how brown adipocytes are distinguished from white adipocytes at the level of translational regulation remains poorly understood. To systemically determine the translational control of gene expression in adipose tissue, we performed ribosome profiling and RNA-seq in parallel to depict the translatome and transcriptome changes during primary brown and white adipogenesis, and between brown and white adipose tissue. The most prominent layer of translational regulation was the increased translation efficiency of genes encoding mitochondria components in brown adipocytes relative to white. Systemic analysis of the regulatory interactions between microRNAs and their targets revealed that microRNAs were more active in repressing targets’ mRNA abundance and translation in brown fat. Together, our data comprehensively delineated a landscape integrating transcriptome and translatome in adipose tissue.David W. ReidDan XuPeng ChenHongyuan YangLei SunNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q David W. Reid Dan Xu Peng Chen Hongyuan Yang Lei Sun Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs
description	Abstract The epidemic of obesity and diabetes has markedly spurred the research interest in adipocyte biology. Brown adipocytes are specialized for energy expenditure and of therapeutic interest for treatment of metabolic diseases, but how brown adipocytes are distinguished from white adipocytes at the level of translational regulation remains poorly understood. To systemically determine the translational control of gene expression in adipose tissue, we performed ribosome profiling and RNA-seq in parallel to depict the translatome and transcriptome changes during primary brown and white adipogenesis, and between brown and white adipose tissue. The most prominent layer of translational regulation was the increased translation efficiency of genes encoding mitochondria components in brown adipocytes relative to white. Systemic analysis of the regulatory interactions between microRNAs and their targets revealed that microRNAs were more active in repressing targets’ mRNA abundance and translation in brown fat. Together, our data comprehensively delineated a landscape integrating transcriptome and translatome in adipose tissue.
format	article
author	David W. Reid Dan Xu Peng Chen Hongyuan Yang Lei Sun
author_facet	David W. Reid Dan Xu Peng Chen Hongyuan Yang Lei Sun
author_sort	David W. Reid
title	Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs
title_short	Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs
title_full	Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs
title_fullStr	Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs
title_full_unstemmed	Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs
title_sort	integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by micrornas
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/42814b23c8b94a7fb4c6fb81551195e8
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Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs

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