BtuB-Dependent Infection of the T5-like <i>Yersinia</i> Phage ϕR2-01

<i>Yersinia enterocolitica</i> is a food-borne Gram-negative pathogen responsible for several gastrointestinal disorders. Host-specific lytic bacteriophages have been increasingly used recently as an alternative or complementary treatment to combat bacterial infections, especially when a...

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Auteurs principaux:	Lotta J. Happonen, Maria I. Pajunen, Jin Woo Jun, Mikael Skurnik
Format:	article
Langue:	EN
Publié:	MDPI AG 2021
Sujets:	bacteriophage phage ϕR2-01 <i>Yersinia enterocolitica</i> <i>Escherichia</i> phage T5 BtuB genome Microbiology QR1-502
Accès en ligne:	https://doaj.org/article/42814fd83c154b54bf4f546311a93d87
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id	oai:doaj.org-article:42814fd83c154b54bf4f546311a93d87
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spelling	oai:doaj.org-article:42814fd83c154b54bf4f546311a93d872021-11-25T19:13:01ZBtuB-Dependent Infection of the T5-like <i>Yersinia</i> Phage ϕR2-0110.3390/v131121711999-4915https://doaj.org/article/42814fd83c154b54bf4f546311a93d872021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2171https://doaj.org/toc/1999-4915<i>Yersinia enterocolitica</i> is a food-borne Gram-negative pathogen responsible for several gastrointestinal disorders. Host-specific lytic bacteriophages have been increasingly used recently as an alternative or complementary treatment to combat bacterial infections, especially when antibiotics fail. Here, we describe the proteogenomic characterization and host receptor identification of the siphovirus vB_YenS_ϕR2-01 (in short, ϕR2-01) that infects strains of several <i>Yersinia enterocolitica</i> serotypes. The ϕR2-01 genome contains 154 predicted genes, 117 of which encode products that are homologous to those of <i>Escherichia</i> bacteriophage T5. The ϕR2-01 and T5 genomes are largely syntenic, with the major differences residing in areas encoding hypothetical ϕR2-01 proteins. Label-free mass-spectrometry-based proteomics confirmed the expression of 90 of the ϕR2-01 genes, with 88 of these being either phage particle structural or phage-particle-associated proteins. In vitro transposon-based host mutagenesis and ϕR2-01 adsorption experiments identified the outer membrane vitamin B12 receptor BtuB as the host receptor. This study provides a proteogenomic characterization of a T5-type bacteriophage and identifies specific <i>Y. enterocolitica</i> strains sensitive to infection with possible future applications of ϕR2-01 as a food biocontrol or phage therapy agent.Lotta J. HapponenMaria I. PajunenJin Woo JunMikael SkurnikMDPI AGarticlebacteriophagephage ϕR2-01<i>Yersinia enterocolitica</i><i>Escherichia</i> phage T5BtuBgenomeMicrobiologyQR1-502ENViruses, Vol 13, Iss 2171, p 2171 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacteriophage phage ϕR2-01 <i>Yersinia enterocolitica</i> <i>Escherichia</i> phage T5 BtuB genome Microbiology QR1-502
spellingShingle	bacteriophage phage ϕR2-01 <i>Yersinia enterocolitica</i> <i>Escherichia</i> phage T5 BtuB genome Microbiology QR1-502 Lotta J. Happonen Maria I. Pajunen Jin Woo Jun Mikael Skurnik BtuB-Dependent Infection of the T5-like <i>Yersinia</i> Phage ϕR2-01
description	<i>Yersinia enterocolitica</i> is a food-borne Gram-negative pathogen responsible for several gastrointestinal disorders. Host-specific lytic bacteriophages have been increasingly used recently as an alternative or complementary treatment to combat bacterial infections, especially when antibiotics fail. Here, we describe the proteogenomic characterization and host receptor identification of the siphovirus vB_YenS_ϕR2-01 (in short, ϕR2-01) that infects strains of several <i>Yersinia enterocolitica</i> serotypes. The ϕR2-01 genome contains 154 predicted genes, 117 of which encode products that are homologous to those of <i>Escherichia</i> bacteriophage T5. The ϕR2-01 and T5 genomes are largely syntenic, with the major differences residing in areas encoding hypothetical ϕR2-01 proteins. Label-free mass-spectrometry-based proteomics confirmed the expression of 90 of the ϕR2-01 genes, with 88 of these being either phage particle structural or phage-particle-associated proteins. In vitro transposon-based host mutagenesis and ϕR2-01 adsorption experiments identified the outer membrane vitamin B12 receptor BtuB as the host receptor. This study provides a proteogenomic characterization of a T5-type bacteriophage and identifies specific <i>Y. enterocolitica</i> strains sensitive to infection with possible future applications of ϕR2-01 as a food biocontrol or phage therapy agent.
format	article
author	Lotta J. Happonen Maria I. Pajunen Jin Woo Jun Mikael Skurnik
author_facet	Lotta J. Happonen Maria I. Pajunen Jin Woo Jun Mikael Skurnik
author_sort	Lotta J. Happonen
title	BtuB-Dependent Infection of the T5-like <i>Yersinia</i> Phage ϕR2-01
title_short	BtuB-Dependent Infection of the T5-like <i>Yersinia</i> Phage ϕR2-01
title_full	BtuB-Dependent Infection of the T5-like <i>Yersinia</i> Phage ϕR2-01
title_fullStr	BtuB-Dependent Infection of the T5-like <i>Yersinia</i> Phage ϕR2-01
title_full_unstemmed	BtuB-Dependent Infection of the T5-like <i>Yersinia</i> Phage ϕR2-01
title_sort	btub-dependent infection of the t5-like <i>yersinia</i> phage ϕr2-01
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/42814fd83c154b54bf4f546311a93d87
work_keys_str_mv	AT lottajhapponen btubdependentinfectionofthet5likeiyersiniaiphagephr201 AT mariaipajunen btubdependentinfectionofthet5likeiyersiniaiphagephr201 AT jinwoojun btubdependentinfectionofthet5likeiyersiniaiphagephr201 AT mikaelskurnik btubdependentinfectionofthet5likeiyersiniaiphagephr201
_version_	1718410150088278016

BtuB-Dependent Infection of the T5-like <i>Yersinia</i> Phage ϕR2-01

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