Long non-coding RNA TRPM2-AS regulates microRNA miR-138-5p and PLAU (Plasminogen Activator, Urokinase) to promote the progression of gastric adenocarcinoma

Gastric adenocarcinoma (GAC) is a common malignant tumor, accounting for 95% of gastric cancers. However, the effects and regulatory mechanisms of long non-coding RNA TRPM2-AS (TRPM2-AS) in GAC have not been fully explored. Our study investigates the action mechanism of TRPM2-AS in GAC. After perfor...

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Autores principales:	Jun Sun, Fang Zhou, Juan Xue, Chunyan Ji, Yinzong Qu, Yuwei Pan
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	trpm2-as mir-138-5p plau gastric adenocarcinoma Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/4281b9463af74afcaa847d76c5c9350b
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spelling	oai:doaj.org-article:4281b9463af74afcaa847d76c5c9350b2021-11-04T15:51:54ZLong non-coding RNA TRPM2-AS regulates microRNA miR-138-5p and PLAU (Plasminogen Activator, Urokinase) to promote the progression of gastric adenocarcinoma2165-59792165-598710.1080/21655979.2021.1995101https://doaj.org/article/4281b9463af74afcaa847d76c5c9350b2021-10-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1995101https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Gastric adenocarcinoma (GAC) is a common malignant tumor, accounting for 95% of gastric cancers. However, the effects and regulatory mechanisms of long non-coding RNA TRPM2-AS (TRPM2-AS) in GAC have not been fully explored. Our study investigates the action mechanism of TRPM2-AS in GAC. After performing quantitative Real-Time polymerase chain reaction or western blotting, we found that the levels of TRPM2-AS and Plasminogen Activator, Urokinase (PLAU) were upregulated in GAC, whereas the level of miR-138-5p was downregulated. Cell function experiments proved that silencing TRPM2-AS suppressed proliferation and migration and induced apoptosis in GAC cells. Bioinformatic analysis and luciferase assay identified the interaction between TRPM2-AS, miR-138-5p, and PLAU. In addition, the inhibitory effect of silencing TRPM2-AS on GAC cells could be partially relieved by PLAU overexpression. In conclusion, our study revealed that TRPM2-AS sponging miR-138-5p to upregulate PLAU could contribute to GAC progression, which might be useful for identifying biomarkers for GAC therapy.Jun SunFang ZhouJuan XueChunyan JiYinzong QuYuwei PanTaylor & Francis Grouparticletrpm2-asmir-138-5pplaugastric adenocarcinomaBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	trpm2-as mir-138-5p plau gastric adenocarcinoma Biotechnology TP248.13-248.65
spellingShingle	trpm2-as mir-138-5p plau gastric adenocarcinoma Biotechnology TP248.13-248.65 Jun Sun Fang Zhou Juan Xue Chunyan Ji Yinzong Qu Yuwei Pan Long non-coding RNA TRPM2-AS regulates microRNA miR-138-5p and PLAU (Plasminogen Activator, Urokinase) to promote the progression of gastric adenocarcinoma
description	Gastric adenocarcinoma (GAC) is a common malignant tumor, accounting for 95% of gastric cancers. However, the effects and regulatory mechanisms of long non-coding RNA TRPM2-AS (TRPM2-AS) in GAC have not been fully explored. Our study investigates the action mechanism of TRPM2-AS in GAC. After performing quantitative Real-Time polymerase chain reaction or western blotting, we found that the levels of TRPM2-AS and Plasminogen Activator, Urokinase (PLAU) were upregulated in GAC, whereas the level of miR-138-5p was downregulated. Cell function experiments proved that silencing TRPM2-AS suppressed proliferation and migration and induced apoptosis in GAC cells. Bioinformatic analysis and luciferase assay identified the interaction between TRPM2-AS, miR-138-5p, and PLAU. In addition, the inhibitory effect of silencing TRPM2-AS on GAC cells could be partially relieved by PLAU overexpression. In conclusion, our study revealed that TRPM2-AS sponging miR-138-5p to upregulate PLAU could contribute to GAC progression, which might be useful for identifying biomarkers for GAC therapy.
format	article
author	Jun Sun Fang Zhou Juan Xue Chunyan Ji Yinzong Qu Yuwei Pan
author_facet	Jun Sun Fang Zhou Juan Xue Chunyan Ji Yinzong Qu Yuwei Pan
author_sort	Jun Sun
title	Long non-coding RNA TRPM2-AS regulates microRNA miR-138-5p and PLAU (Plasminogen Activator, Urokinase) to promote the progression of gastric adenocarcinoma
title_short	Long non-coding RNA TRPM2-AS regulates microRNA miR-138-5p and PLAU (Plasminogen Activator, Urokinase) to promote the progression of gastric adenocarcinoma
title_full	Long non-coding RNA TRPM2-AS regulates microRNA miR-138-5p and PLAU (Plasminogen Activator, Urokinase) to promote the progression of gastric adenocarcinoma
title_fullStr	Long non-coding RNA TRPM2-AS regulates microRNA miR-138-5p and PLAU (Plasminogen Activator, Urokinase) to promote the progression of gastric adenocarcinoma
title_full_unstemmed	Long non-coding RNA TRPM2-AS regulates microRNA miR-138-5p and PLAU (Plasminogen Activator, Urokinase) to promote the progression of gastric adenocarcinoma
title_sort	long non-coding rna trpm2-as regulates microrna mir-138-5p and plau (plasminogen activator, urokinase) to promote the progression of gastric adenocarcinoma
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/4281b9463af74afcaa847d76c5c9350b
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Long non-coding RNA TRPM2-AS regulates microRNA miR-138-5p and PLAU (Plasminogen Activator, Urokinase) to promote the progression of gastric adenocarcinoma

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