Repair of segmental bone defect using tissue engineered heterogeneous deproteinized bone doped with lithium

Abstract Lithium have been shown to play an important role in improving the osteogenic properties of biomaterials. This study aims to explore the osteogenic improvement effect of tissue engineered heterogeneous deproteinized bone (HDPB) doped with lithium, and evaluate their effectiveness in the hea...

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Autores principales:	Jun Li, Wenzhao Wang, Mingxin Li, Lei Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/428232a4142b4dd09e42a47b06d82a56
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spelling	oai:doaj.org-article:428232a4142b4dd09e42a47b06d82a562021-12-02T13:19:22ZRepair of segmental bone defect using tissue engineered heterogeneous deproteinized bone doped with lithium10.1038/s41598-021-84526-w2045-2322https://doaj.org/article/428232a4142b4dd09e42a47b06d82a562021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84526-whttps://doaj.org/toc/2045-2322Abstract Lithium have been shown to play an important role in improving the osteogenic properties of biomaterials. This study aims to explore the osteogenic improvement effect of tissue engineered heterogeneous deproteinized bone (HDPB) doped with lithium, and evaluate their effectiveness in the healing of bone defects. Bone marrow mesenchymal stem cells (BMSCs) were co-cultured with different concentration of lithium chloride. Cell proliferation in each group was analyzed by 3-(4, 5-dimetyl-2-thiazoly-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) assay. BMSCs were then co-cultured in osteogenic induction medium with different concentration of lithium chloride, and the expression of related mRNA was detected. The role of lithium in promoting BMSCs osteogenic differentiation and inhibiting BMSCs lipogenic differentiation was also investigated. Biomechanical properties of the tibia were evaluated at 8 weeks after operation. The tibial specimens of each group were collected at 4 and 8 weeks after surgery for histological examination and histological analysis. Micro-computed tomography (CT) scanning and 3D reconstruction were performed at 8 weeks. The results demonstrate that lithium can induce the osteogenic differentiation inhibit of adipogenic differentiation of BMSCs by regulating the Wnt signaling pathway. The histological evaluation further certified that average bone formation area in the group of tissue engineered HDPB doped with lithium was also significantly better than that of HDPB alone group. Based on the above evaluation, tissue engineered HDPB doped with lithium can effectively promote the regeneration of segmental bone defect, which can be used as a tissue engineering scaffold for clinical trials.Jun LiWenzhao WangMingxin LiLei LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jun Li Wenzhao Wang Mingxin Li Lei Liu Repair of segmental bone defect using tissue engineered heterogeneous deproteinized bone doped with lithium
description	Abstract Lithium have been shown to play an important role in improving the osteogenic properties of biomaterials. This study aims to explore the osteogenic improvement effect of tissue engineered heterogeneous deproteinized bone (HDPB) doped with lithium, and evaluate their effectiveness in the healing of bone defects. Bone marrow mesenchymal stem cells (BMSCs) were co-cultured with different concentration of lithium chloride. Cell proliferation in each group was analyzed by 3-(4, 5-dimetyl-2-thiazoly-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) assay. BMSCs were then co-cultured in osteogenic induction medium with different concentration of lithium chloride, and the expression of related mRNA was detected. The role of lithium in promoting BMSCs osteogenic differentiation and inhibiting BMSCs lipogenic differentiation was also investigated. Biomechanical properties of the tibia were evaluated at 8 weeks after operation. The tibial specimens of each group were collected at 4 and 8 weeks after surgery for histological examination and histological analysis. Micro-computed tomography (CT) scanning and 3D reconstruction were performed at 8 weeks. The results demonstrate that lithium can induce the osteogenic differentiation inhibit of adipogenic differentiation of BMSCs by regulating the Wnt signaling pathway. The histological evaluation further certified that average bone formation area in the group of tissue engineered HDPB doped with lithium was also significantly better than that of HDPB alone group. Based on the above evaluation, tissue engineered HDPB doped with lithium can effectively promote the regeneration of segmental bone defect, which can be used as a tissue engineering scaffold for clinical trials.
format	article
author	Jun Li Wenzhao Wang Mingxin Li Lei Liu
author_facet	Jun Li Wenzhao Wang Mingxin Li Lei Liu
author_sort	Jun Li
title	Repair of segmental bone defect using tissue engineered heterogeneous deproteinized bone doped with lithium
title_short	Repair of segmental bone defect using tissue engineered heterogeneous deproteinized bone doped with lithium
title_full	Repair of segmental bone defect using tissue engineered heterogeneous deproteinized bone doped with lithium
title_fullStr	Repair of segmental bone defect using tissue engineered heterogeneous deproteinized bone doped with lithium
title_full_unstemmed	Repair of segmental bone defect using tissue engineered heterogeneous deproteinized bone doped with lithium
title_sort	repair of segmental bone defect using tissue engineered heterogeneous deproteinized bone doped with lithium
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/428232a4142b4dd09e42a47b06d82a56
work_keys_str_mv	AT junli repairofsegmentalbonedefectusingtissueengineeredheterogeneousdeproteinizedbonedopedwithlithium AT wenzhaowang repairofsegmentalbonedefectusingtissueengineeredheterogeneousdeproteinizedbonedopedwithlithium AT mingxinli repairofsegmentalbonedefectusingtissueengineeredheterogeneousdeproteinizedbonedopedwithlithium AT leiliu repairofsegmentalbonedefectusingtissueengineeredheterogeneousdeproteinizedbonedopedwithlithium
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Repair of segmental bone defect using tissue engineered heterogeneous deproteinized bone doped with lithium

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