Comparison of the amniotic fluid and fetal urine peptidome for biomarker discovery in renal developmental disease

Abstract Production of amniotic fluid (AF) is view as predominately driven by excretion of fetal urine (FU). However, the origin of AF peptides, often considered as potential biomarkers of developmental diseases, has never been investigated. Here, we evaluated the FU origin of AF peptides and if the...

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Autores principales:	Camille Fédou, Benjamin Breuil, Igor Golovko, Stéphane Decramer, Pedro Magalhães, Françoise Muller, Sophie Dreux, Petra Zürbig, Julie Klein, Joost P. Schanstra, Bénédicte Buffin-Meyer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/428622a5f2e3402e83de58ba284e7d13
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spelling	oai:doaj.org-article:428622a5f2e3402e83de58ba284e7d132021-12-02T15:11:50ZComparison of the amniotic fluid and fetal urine peptidome for biomarker discovery in renal developmental disease10.1038/s41598-020-78730-32045-2322https://doaj.org/article/428622a5f2e3402e83de58ba284e7d132020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78730-3https://doaj.org/toc/2045-2322Abstract Production of amniotic fluid (AF) is view as predominately driven by excretion of fetal urine (FU). However, the origin of AF peptides, often considered as potential biomarkers of developmental diseases, has never been investigated. Here, we evaluated the FU origin of AF peptides and if the AF peptide content can be used as a surrogate of FU. The abundance of endogenous peptides was analyzed by capillary electrophoresis coupled to mass spectrometry in 216 AF and 64 FU samples. A total of 2668 and 3257 peptides was found in AF and FU respectively. The AF peptidome largely overlapped with the FU peptidome, ranging from 54% in the second pregnancy trimester to 65% in the third trimester. Examination of a subset of 16 paired AF and FU samples revealed that 67 peptides displayed a significant positively correlated abundance in AF and FU, strongly suggesting that their presence in AF was directly associated to FU excretion. As proof-of-concept we showed that measuring the AF abundance of these 67 peptides of FU origin allowed prediction of postnatal renal survival in fetuses with posterior urethral valves. These results demonstrate that the AF peptidome can be considered as a good surrogate of the FU peptidome.Camille FédouBenjamin BreuilIgor GolovkoStéphane DecramerPedro MagalhãesFrançoise MullerSophie DreuxPetra ZürbigJulie KleinJoost P. SchanstraBénédicte Buffin-MeyerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Camille Fédou Benjamin Breuil Igor Golovko Stéphane Decramer Pedro Magalhães Françoise Muller Sophie Dreux Petra Zürbig Julie Klein Joost P. Schanstra Bénédicte Buffin-Meyer Comparison of the amniotic fluid and fetal urine peptidome for biomarker discovery in renal developmental disease
description	Abstract Production of amniotic fluid (AF) is view as predominately driven by excretion of fetal urine (FU). However, the origin of AF peptides, often considered as potential biomarkers of developmental diseases, has never been investigated. Here, we evaluated the FU origin of AF peptides and if the AF peptide content can be used as a surrogate of FU. The abundance of endogenous peptides was analyzed by capillary electrophoresis coupled to mass spectrometry in 216 AF and 64 FU samples. A total of 2668 and 3257 peptides was found in AF and FU respectively. The AF peptidome largely overlapped with the FU peptidome, ranging from 54% in the second pregnancy trimester to 65% in the third trimester. Examination of a subset of 16 paired AF and FU samples revealed that 67 peptides displayed a significant positively correlated abundance in AF and FU, strongly suggesting that their presence in AF was directly associated to FU excretion. As proof-of-concept we showed that measuring the AF abundance of these 67 peptides of FU origin allowed prediction of postnatal renal survival in fetuses with posterior urethral valves. These results demonstrate that the AF peptidome can be considered as a good surrogate of the FU peptidome.
format	article
author	Camille Fédou Benjamin Breuil Igor Golovko Stéphane Decramer Pedro Magalhães Françoise Muller Sophie Dreux Petra Zürbig Julie Klein Joost P. Schanstra Bénédicte Buffin-Meyer
author_facet	Camille Fédou Benjamin Breuil Igor Golovko Stéphane Decramer Pedro Magalhães Françoise Muller Sophie Dreux Petra Zürbig Julie Klein Joost P. Schanstra Bénédicte Buffin-Meyer
author_sort	Camille Fédou
title	Comparison of the amniotic fluid and fetal urine peptidome for biomarker discovery in renal developmental disease
title_short	Comparison of the amniotic fluid and fetal urine peptidome for biomarker discovery in renal developmental disease
title_full	Comparison of the amniotic fluid and fetal urine peptidome for biomarker discovery in renal developmental disease
title_fullStr	Comparison of the amniotic fluid and fetal urine peptidome for biomarker discovery in renal developmental disease
title_full_unstemmed	Comparison of the amniotic fluid and fetal urine peptidome for biomarker discovery in renal developmental disease
title_sort	comparison of the amniotic fluid and fetal urine peptidome for biomarker discovery in renal developmental disease
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/428622a5f2e3402e83de58ba284e7d13
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Comparison of the amniotic fluid and fetal urine peptidome for biomarker discovery in renal developmental disease

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