A Ready-to-Use Metal-Supported Bilayer Lipid Membrane Biosensor for the Detection of Phenol in Water

This work presents a novel metal-supported bilayer lipid membrane (BLM) biosensor built on tyrosinase to quantitate phenol. The detection strategy is based on the enzyme–analyte initial association and not the commonly adopted monitoring of the redox cascade reactions; such an approach has not been...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Christina G. Siontorou, Konstantinos N. Georgopoulos
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
BLM
Acceso en línea:https://doaj.org/article/428ad778216e452496b7c7e24765c197
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
Descripción
Sumario:This work presents a novel metal-supported bilayer lipid membrane (BLM) biosensor built on tyrosinase to quantitate phenol. The detection strategy is based on the enzyme–analyte initial association and not the commonly adopted monitoring of the redox cascade reactions; such an approach has not been proposed in the literature to date and offers many advantages for environmental monitoring with regard to sensitivity, selectivity, reliability and assay simplicity. The phenol sensor developed herein showed good analytical and operational characteristics: the detection limit (signal-to-noise ratio = 3) was 1.24 pg/mL and the sensitivity was 33.45 nA per pg/mL phenol concentration. The shelf life of the tyrosinase sensor was 12 h and the lifetime (in consecutive assays) was 8 h. The sensor was reversible with bathing at pH 8.5 and could be used for eight assay runs in consecutive assays. The validation in real water samples showed that the sensor could reliably detect 2.5 ppb phenol in tap and river water and 6.1 ppb phenol in lake water, without sample pretreatment. The prospects and applicability of the proposed biosensor and the underlying technology are also discussed.