A Ready-to-Use Metal-Supported Bilayer Lipid Membrane Biosensor for the Detection of Phenol in Water

This work presents a novel metal-supported bilayer lipid membrane (BLM) biosensor built on tyrosinase to quantitate phenol. The detection strategy is based on the enzyme–analyte initial association and not the commonly adopted monitoring of the redox cascade reactions; such an approach has not been...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Christina G. Siontorou, Konstantinos N. Georgopoulos
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	BLM metal-supported biosensor self-assembly tyrosinase water monitoring Chemical technology TP1-1185 Chemical engineering TP155-156
Acceso en línea:	https://doaj.org/article/428ad778216e452496b7c7e24765c197
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:428ad778216e452496b7c7e24765c197
record_format	dspace
spelling	oai:doaj.org-article:428ad778216e452496b7c7e24765c1972021-11-25T18:19:57ZA Ready-to-Use Metal-Supported Bilayer Lipid Membrane Biosensor for the Detection of Phenol in Water10.3390/membranes111108712077-0375https://doaj.org/article/428ad778216e452496b7c7e24765c1972021-11-01T00:00:00Zhttps://www.mdpi.com/2077-0375/11/11/871https://doaj.org/toc/2077-0375This work presents a novel metal-supported bilayer lipid membrane (BLM) biosensor built on tyrosinase to quantitate phenol. The detection strategy is based on the enzyme–analyte initial association and not the commonly adopted monitoring of the redox cascade reactions; such an approach has not been proposed in the literature to date and offers many advantages for environmental monitoring with regard to sensitivity, selectivity, reliability and assay simplicity. The phenol sensor developed herein showed good analytical and operational characteristics: the detection limit (signal-to-noise ratio = 3) was 1.24 pg/mL and the sensitivity was 33.45 nA per pg/mL phenol concentration. The shelf life of the tyrosinase sensor was 12 h and the lifetime (in consecutive assays) was 8 h. The sensor was reversible with bathing at pH 8.5 and could be used for eight assay runs in consecutive assays. The validation in real water samples showed that the sensor could reliably detect 2.5 ppb phenol in tap and river water and 6.1 ppb phenol in lake water, without sample pretreatment. The prospects and applicability of the proposed biosensor and the underlying technology are also discussed.Christina G. SiontorouKonstantinos N. GeorgopoulosMDPI AGarticleBLMmetal-supportedbiosensorself-assemblytyrosinasewater monitoringChemical technologyTP1-1185Chemical engineeringTP155-156ENMembranes, Vol 11, Iss 871, p 871 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	BLM metal-supported biosensor self-assembly tyrosinase water monitoring Chemical technology TP1-1185 Chemical engineering TP155-156
spellingShingle	BLM metal-supported biosensor self-assembly tyrosinase water monitoring Chemical technology TP1-1185 Chemical engineering TP155-156 Christina G. Siontorou Konstantinos N. Georgopoulos A Ready-to-Use Metal-Supported Bilayer Lipid Membrane Biosensor for the Detection of Phenol in Water
description	This work presents a novel metal-supported bilayer lipid membrane (BLM) biosensor built on tyrosinase to quantitate phenol. The detection strategy is based on the enzyme–analyte initial association and not the commonly adopted monitoring of the redox cascade reactions; such an approach has not been proposed in the literature to date and offers many advantages for environmental monitoring with regard to sensitivity, selectivity, reliability and assay simplicity. The phenol sensor developed herein showed good analytical and operational characteristics: the detection limit (signal-to-noise ratio = 3) was 1.24 pg/mL and the sensitivity was 33.45 nA per pg/mL phenol concentration. The shelf life of the tyrosinase sensor was 12 h and the lifetime (in consecutive assays) was 8 h. The sensor was reversible with bathing at pH 8.5 and could be used for eight assay runs in consecutive assays. The validation in real water samples showed that the sensor could reliably detect 2.5 ppb phenol in tap and river water and 6.1 ppb phenol in lake water, without sample pretreatment. The prospects and applicability of the proposed biosensor and the underlying technology are also discussed.
format	article
author	Christina G. Siontorou Konstantinos N. Georgopoulos
author_facet	Christina G. Siontorou Konstantinos N. Georgopoulos
author_sort	Christina G. Siontorou
title	A Ready-to-Use Metal-Supported Bilayer Lipid Membrane Biosensor for the Detection of Phenol in Water
title_short	A Ready-to-Use Metal-Supported Bilayer Lipid Membrane Biosensor for the Detection of Phenol in Water
title_full	A Ready-to-Use Metal-Supported Bilayer Lipid Membrane Biosensor for the Detection of Phenol in Water
title_fullStr	A Ready-to-Use Metal-Supported Bilayer Lipid Membrane Biosensor for the Detection of Phenol in Water
title_full_unstemmed	A Ready-to-Use Metal-Supported Bilayer Lipid Membrane Biosensor for the Detection of Phenol in Water
title_sort	ready-to-use metal-supported bilayer lipid membrane biosensor for the detection of phenol in water
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/428ad778216e452496b7c7e24765c197
work_keys_str_mv	AT christinagsiontorou areadytousemetalsupportedbilayerlipidmembranebiosensorforthedetectionofphenolinwater AT konstantinosngeorgopoulos areadytousemetalsupportedbilayerlipidmembranebiosensorforthedetectionofphenolinwater AT christinagsiontorou readytousemetalsupportedbilayerlipidmembranebiosensorforthedetectionofphenolinwater AT konstantinosngeorgopoulos readytousemetalsupportedbilayerlipidmembranebiosensorforthedetectionofphenolinwater
_version_	1718411315028951040

A Ready-to-Use Metal-Supported Bilayer Lipid Membrane Biosensor for the Detection of Phenol in Water

Ejemplares similares