Natural variation in the regulation of neurodevelopmental genes modifies flight performance in Drosophila.

The winged insects of the order Diptera are colloquially named for their most recognizable phenotype: flight. These insects rely on flight for a number of important life history traits, such as dispersal, foraging, and courtship. Despite the importance of flight, relatively little is known about the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Adam N Spierer, Jim A Mossman, Samuel Pattillo Smith, Lorin Crawford, Sohini Ramachandran, David M Rand
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/4295191819b24473bf872086bdb9c275
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4295191819b24473bf872086bdb9c275
record_format	dspace
spelling	oai:doaj.org-article:4295191819b24473bf872086bdb9c2752021-12-02T20:02:58ZNatural variation in the regulation of neurodevelopmental genes modifies flight performance in Drosophila.1553-73901553-740410.1371/journal.pgen.1008887https://doaj.org/article/4295191819b24473bf872086bdb9c2752021-03-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1008887https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404The winged insects of the order Diptera are colloquially named for their most recognizable phenotype: flight. These insects rely on flight for a number of important life history traits, such as dispersal, foraging, and courtship. Despite the importance of flight, relatively little is known about the genetic architecture of flight performance. Accordingly, we sought to uncover the genetic modifiers of flight using a measure of flies' reaction and response to an abrupt drop in a vertical flight column. We conducted a genome wide association study (GWAS) using 197 of the Drosophila Genetic Reference Panel (DGRP) lines, and identified a combination of additive and marginal variants, epistatic interactions, whole genes, and enrichment across interaction networks. Egfr, a highly pleiotropic developmental gene, was among the most significant additive variants identified. We functionally validated 13 of the additive candidate genes' (Adgf-A/Adgf-A2/CG32181, bru1, CadN, flapper (CG11073), CG15236, flippy (CG9766), CREG, Dscam4, form3, fry, Lasp/CG9692, Pde6, Snoo), and introduce a novel approach to whole gene significance screens: PEGASUS_flies. Additionally, we identified ppk23, an Acid Sensing Ion Channel (ASIC) homolog, as an important hub for epistatic interactions. We propose a model that suggests genetic modifiers of wing and muscle morphology, nervous system development and function, BMP signaling, sexually dimorphic neural wiring, and gene regulation are all important for the observed differences flight performance in a natural population. Additionally, these results represent a snapshot of the genetic modifiers affecting drop-response flight performance in Drosophila, with implications for other insects.Adam N SpiererJim A MossmanSamuel Pattillo SmithLorin CrawfordSohini RamachandranDavid M RandPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 3, p e1008887 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Adam N Spierer Jim A Mossman Samuel Pattillo Smith Lorin Crawford Sohini Ramachandran David M Rand Natural variation in the regulation of neurodevelopmental genes modifies flight performance in Drosophila.
description	The winged insects of the order Diptera are colloquially named for their most recognizable phenotype: flight. These insects rely on flight for a number of important life history traits, such as dispersal, foraging, and courtship. Despite the importance of flight, relatively little is known about the genetic architecture of flight performance. Accordingly, we sought to uncover the genetic modifiers of flight using a measure of flies' reaction and response to an abrupt drop in a vertical flight column. We conducted a genome wide association study (GWAS) using 197 of the Drosophila Genetic Reference Panel (DGRP) lines, and identified a combination of additive and marginal variants, epistatic interactions, whole genes, and enrichment across interaction networks. Egfr, a highly pleiotropic developmental gene, was among the most significant additive variants identified. We functionally validated 13 of the additive candidate genes' (Adgf-A/Adgf-A2/CG32181, bru1, CadN, flapper (CG11073), CG15236, flippy (CG9766), CREG, Dscam4, form3, fry, Lasp/CG9692, Pde6, Snoo), and introduce a novel approach to whole gene significance screens: PEGASUS_flies. Additionally, we identified ppk23, an Acid Sensing Ion Channel (ASIC) homolog, as an important hub for epistatic interactions. We propose a model that suggests genetic modifiers of wing and muscle morphology, nervous system development and function, BMP signaling, sexually dimorphic neural wiring, and gene regulation are all important for the observed differences flight performance in a natural population. Additionally, these results represent a snapshot of the genetic modifiers affecting drop-response flight performance in Drosophila, with implications for other insects.
format	article
author	Adam N Spierer Jim A Mossman Samuel Pattillo Smith Lorin Crawford Sohini Ramachandran David M Rand
author_facet	Adam N Spierer Jim A Mossman Samuel Pattillo Smith Lorin Crawford Sohini Ramachandran David M Rand
author_sort	Adam N Spierer
title	Natural variation in the regulation of neurodevelopmental genes modifies flight performance in Drosophila.
title_short	Natural variation in the regulation of neurodevelopmental genes modifies flight performance in Drosophila.
title_full	Natural variation in the regulation of neurodevelopmental genes modifies flight performance in Drosophila.
title_fullStr	Natural variation in the regulation of neurodevelopmental genes modifies flight performance in Drosophila.
title_full_unstemmed	Natural variation in the regulation of neurodevelopmental genes modifies flight performance in Drosophila.
title_sort	natural variation in the regulation of neurodevelopmental genes modifies flight performance in drosophila.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/4295191819b24473bf872086bdb9c275
work_keys_str_mv	AT adamnspierer naturalvariationintheregulationofneurodevelopmentalgenesmodifiesflightperformanceindrosophila AT jimamossman naturalvariationintheregulationofneurodevelopmentalgenesmodifiesflightperformanceindrosophila AT samuelpattillosmith naturalvariationintheregulationofneurodevelopmentalgenesmodifiesflightperformanceindrosophila AT lorincrawford naturalvariationintheregulationofneurodevelopmentalgenesmodifiesflightperformanceindrosophila AT sohiniramachandran naturalvariationintheregulationofneurodevelopmentalgenesmodifiesflightperformanceindrosophila AT davidmrand naturalvariationintheregulationofneurodevelopmentalgenesmodifiesflightperformanceindrosophila
_version_	1718375655158054912

Natural variation in the regulation of neurodevelopmental genes modifies flight performance in Drosophila.

Ejemplares similares