A novel signature for stratifying the molecular heterogeneity of the tissue-infiltrating T-cell receptor repertoire reflects gastric cancer prognosis
Abstract Many basic properties of the T-cell receptor (TCR) repertoire require clarification, and the changes occurring in the TCR repertoire during carcinogenesis, especially during precancerous stages, remain unclear. This study used deep sequencing analyses to examine 41 gastric tissue samples at...
Guardado en:
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2017
|
Materias: | |
Acceso en línea: | https://doaj.org/article/429c8b47cda445968aacff01c6f16875 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:429c8b47cda445968aacff01c6f16875 |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:429c8b47cda445968aacff01c6f168752021-12-02T15:06:15ZA novel signature for stratifying the molecular heterogeneity of the tissue-infiltrating T-cell receptor repertoire reflects gastric cancer prognosis10.1038/s41598-017-08289-z2045-2322https://doaj.org/article/429c8b47cda445968aacff01c6f168752017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08289-zhttps://doaj.org/toc/2045-2322Abstract Many basic properties of the T-cell receptor (TCR) repertoire require clarification, and the changes occurring in the TCR repertoire during carcinogenesis, especially during precancerous stages, remain unclear. This study used deep sequencing analyses to examine 41 gastric tissue samples at different pathological stages, including low-grade intraepithelial neoplasia, high-grade intraepithelial neoplasia, early gastric cancer and matched adjacent tissues, to define the characteristics of the infiltrating TCRβ repertoire during gastric carcinogenesis. Moreover, to illustrate the relationship between the local molecular phenotype and TCR repertoire of the microenvironment, whole-genome gene expression microarray analysis of the corresponding gastric precancerous lesions and early gastric cancer tissues was conducted. Our results showed that the degree of variation in the TCR repertoire gradually increased during tumourigenesis. Integrative analysis of microarray data and the TCR repertoire variation index using the network-based Clique Percolation Method identified an 11-gene module related to the inflammatory response that can predict the overall survival of gastric cancer (GC) patients. In conclusion, our results revealed the multistage heterogeneity of tissue-infiltrating TCR repertoire during carcinogenesis. We report a novel way for identifying prognostic biomarkers for GC patients and improves our understanding of immune responses during gastric carcinogenesis.Manchao KuangJieyao ChengChengli ZhangLin FengXue XuYajing ZhangMing ZuJianfang CuiHang YuKaitai ZhangAiming YangShujun ChengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Manchao Kuang Jieyao Cheng Chengli Zhang Lin Feng Xue Xu Yajing Zhang Ming Zu Jianfang Cui Hang Yu Kaitai Zhang Aiming Yang Shujun Cheng A novel signature for stratifying the molecular heterogeneity of the tissue-infiltrating T-cell receptor repertoire reflects gastric cancer prognosis |
description |
Abstract Many basic properties of the T-cell receptor (TCR) repertoire require clarification, and the changes occurring in the TCR repertoire during carcinogenesis, especially during precancerous stages, remain unclear. This study used deep sequencing analyses to examine 41 gastric tissue samples at different pathological stages, including low-grade intraepithelial neoplasia, high-grade intraepithelial neoplasia, early gastric cancer and matched adjacent tissues, to define the characteristics of the infiltrating TCRβ repertoire during gastric carcinogenesis. Moreover, to illustrate the relationship between the local molecular phenotype and TCR repertoire of the microenvironment, whole-genome gene expression microarray analysis of the corresponding gastric precancerous lesions and early gastric cancer tissues was conducted. Our results showed that the degree of variation in the TCR repertoire gradually increased during tumourigenesis. Integrative analysis of microarray data and the TCR repertoire variation index using the network-based Clique Percolation Method identified an 11-gene module related to the inflammatory response that can predict the overall survival of gastric cancer (GC) patients. In conclusion, our results revealed the multistage heterogeneity of tissue-infiltrating TCR repertoire during carcinogenesis. We report a novel way for identifying prognostic biomarkers for GC patients and improves our understanding of immune responses during gastric carcinogenesis. |
format |
article |
author |
Manchao Kuang Jieyao Cheng Chengli Zhang Lin Feng Xue Xu Yajing Zhang Ming Zu Jianfang Cui Hang Yu Kaitai Zhang Aiming Yang Shujun Cheng |
author_facet |
Manchao Kuang Jieyao Cheng Chengli Zhang Lin Feng Xue Xu Yajing Zhang Ming Zu Jianfang Cui Hang Yu Kaitai Zhang Aiming Yang Shujun Cheng |
author_sort |
Manchao Kuang |
title |
A novel signature for stratifying the molecular heterogeneity of the tissue-infiltrating T-cell receptor repertoire reflects gastric cancer prognosis |
title_short |
A novel signature for stratifying the molecular heterogeneity of the tissue-infiltrating T-cell receptor repertoire reflects gastric cancer prognosis |
title_full |
A novel signature for stratifying the molecular heterogeneity of the tissue-infiltrating T-cell receptor repertoire reflects gastric cancer prognosis |
title_fullStr |
A novel signature for stratifying the molecular heterogeneity of the tissue-infiltrating T-cell receptor repertoire reflects gastric cancer prognosis |
title_full_unstemmed |
A novel signature for stratifying the molecular heterogeneity of the tissue-infiltrating T-cell receptor repertoire reflects gastric cancer prognosis |
title_sort |
novel signature for stratifying the molecular heterogeneity of the tissue-infiltrating t-cell receptor repertoire reflects gastric cancer prognosis |
publisher |
Nature Portfolio |
publishDate |
2017 |
url |
https://doaj.org/article/429c8b47cda445968aacff01c6f16875 |
work_keys_str_mv |
AT manchaokuang anovelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT jieyaocheng anovelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT chenglizhang anovelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT linfeng anovelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT xuexu anovelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT yajingzhang anovelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT mingzu anovelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT jianfangcui anovelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT hangyu anovelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT kaitaizhang anovelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT aimingyang anovelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT shujuncheng anovelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT manchaokuang novelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT jieyaocheng novelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT chenglizhang novelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT linfeng novelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT xuexu novelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT yajingzhang novelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT mingzu novelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT jianfangcui novelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT hangyu novelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT kaitaizhang novelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT aimingyang novelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis AT shujuncheng novelsignatureforstratifyingthemolecularheterogeneityofthetissueinfiltratingtcellreceptorrepertoirereflectsgastriccancerprognosis |
_version_ |
1718388507801550848 |