Synergy effects of Asperosaponin VI and bioactive factor BMP-2 on osteogenesis and anti-osteoclastogenesis

Osteoporosis is a reduction in skeletal mass due to the decrease of osteogenic ability and the activation of the osteoclastic function. Inhibiting bone resorption and accelerating the new bone formation is a promising strategy to repair the bone defect of osteoporosis. In this study, we first system...

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Autores principales:	Fangping Chen, Qing Liang, Lijie Mao, Yanrong Yin, Lixin Zhang, Cuidi Li, Changsheng Liu
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	Asperosaponin VI rhBMP-2 Osteoporosis Osteogenesis Osteoclastogenesis Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/429f9f6059cd4467b1b5ed9c49605eef
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spelling	oai:doaj.org-article:429f9f6059cd4467b1b5ed9c49605eef2021-11-28T04:35:25ZSynergy effects of Asperosaponin VI and bioactive factor BMP-2 on osteogenesis and anti-osteoclastogenesis2452-199X10.1016/j.bioactmat.2021.09.001https://doaj.org/article/429f9f6059cd4467b1b5ed9c49605eef2022-04-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2452199X21004114https://doaj.org/toc/2452-199XOsteoporosis is a reduction in skeletal mass due to the decrease of osteogenic ability and the activation of the osteoclastic function. Inhibiting bone resorption and accelerating the new bone formation is a promising strategy to repair the bone defect of osteoporosis. In this study, we first systematically investigated the roles of Chinese medicine Asperosaponin VI (ASP VI) on osteogenic mineralization of BMSCs and osteoclastogenesis of BMMs, and then explored the synergistic effect of ASP VI and BS (BMP-2 immobilized in 2-N, 6-O-sulfated chitosan) on bone formation. The result showed that ASP VI with the concentration lower than 10−4 M contributed to the expression of osteogenic gene and inhibited osteoclastic genes RANKL of BMSCs. Simultaneously, ASP VI significantly reduced the differentiation of mononuclear osteoclasts in the process of osteoclast formation induced by M-CSF and RANKL. Furthermore, by stimulating the SMADs, TGF-β1, VEGFA, and OPG/RANKL signaling pathways, ASBS (ASP VI and BS) substantially enhanced osteogenesis, greatly promoted angiogenesis, and suppressed osteoclastogenesis. The findings provide a new perspective on osteoporosis care and prevention.Fangping ChenQing LiangLijie MaoYanrong YinLixin ZhangCuidi LiChangsheng LiuKeAi Communications Co., Ltd.articleAsperosaponin VIrhBMP-2OsteoporosisOsteogenesisOsteoclastogenesisMaterials of engineering and construction. Mechanics of materialsTA401-492Biology (General)QH301-705.5ENBioactive Materials, Vol 10, Iss , Pp 335-344 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Asperosaponin VI rhBMP-2 Osteoporosis Osteogenesis Osteoclastogenesis Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
spellingShingle	Asperosaponin VI rhBMP-2 Osteoporosis Osteogenesis Osteoclastogenesis Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5 Fangping Chen Qing Liang Lijie Mao Yanrong Yin Lixin Zhang Cuidi Li Changsheng Liu Synergy effects of Asperosaponin VI and bioactive factor BMP-2 on osteogenesis and anti-osteoclastogenesis
description	Osteoporosis is a reduction in skeletal mass due to the decrease of osteogenic ability and the activation of the osteoclastic function. Inhibiting bone resorption and accelerating the new bone formation is a promising strategy to repair the bone defect of osteoporosis. In this study, we first systematically investigated the roles of Chinese medicine Asperosaponin VI (ASP VI) on osteogenic mineralization of BMSCs and osteoclastogenesis of BMMs, and then explored the synergistic effect of ASP VI and BS (BMP-2 immobilized in 2-N, 6-O-sulfated chitosan) on bone formation. The result showed that ASP VI with the concentration lower than 10−4 M contributed to the expression of osteogenic gene and inhibited osteoclastic genes RANKL of BMSCs. Simultaneously, ASP VI significantly reduced the differentiation of mononuclear osteoclasts in the process of osteoclast formation induced by M-CSF and RANKL. Furthermore, by stimulating the SMADs, TGF-β1, VEGFA, and OPG/RANKL signaling pathways, ASBS (ASP VI and BS) substantially enhanced osteogenesis, greatly promoted angiogenesis, and suppressed osteoclastogenesis. The findings provide a new perspective on osteoporosis care and prevention.
format	article
author	Fangping Chen Qing Liang Lijie Mao Yanrong Yin Lixin Zhang Cuidi Li Changsheng Liu
author_facet	Fangping Chen Qing Liang Lijie Mao Yanrong Yin Lixin Zhang Cuidi Li Changsheng Liu
author_sort	Fangping Chen
title	Synergy effects of Asperosaponin VI and bioactive factor BMP-2 on osteogenesis and anti-osteoclastogenesis
title_short	Synergy effects of Asperosaponin VI and bioactive factor BMP-2 on osteogenesis and anti-osteoclastogenesis
title_full	Synergy effects of Asperosaponin VI and bioactive factor BMP-2 on osteogenesis and anti-osteoclastogenesis
title_fullStr	Synergy effects of Asperosaponin VI and bioactive factor BMP-2 on osteogenesis and anti-osteoclastogenesis
title_full_unstemmed	Synergy effects of Asperosaponin VI and bioactive factor BMP-2 on osteogenesis and anti-osteoclastogenesis
title_sort	synergy effects of asperosaponin vi and bioactive factor bmp-2 on osteogenesis and anti-osteoclastogenesis
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/429f9f6059cd4467b1b5ed9c49605eef
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Synergy effects of Asperosaponin VI and bioactive factor BMP-2 on osteogenesis and anti-osteoclastogenesis

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