Endophyte microbiome diversity in micropropagated Atriplex canescens and Atriplex torreyi var griffithsii.

Microbial diversity associated with micropropagated Atriplex species was assessed using microscopy, isolate culturing, and sequencing. Light, electron, and confocal microscopy revealed microbial cells in aseptically regenerated leaves and roots. Clone libraries and tag-encoded FLX amplicon pyroseque...

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Autores principales:	Mary E Lucero, Adrian Unc, Peter Cooke, Scot Dowd, Shulei Sun
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/42a180c1478648508366e5096dccbd2e
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spelling	oai:doaj.org-article:42a180c1478648508366e5096dccbd2e2021-11-18T06:57:13ZEndophyte microbiome diversity in micropropagated Atriplex canescens and Atriplex torreyi var griffithsii.1932-620310.1371/journal.pone.0017693https://doaj.org/article/42a180c1478648508366e5096dccbd2e2011-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21437280/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Microbial diversity associated with micropropagated Atriplex species was assessed using microscopy, isolate culturing, and sequencing. Light, electron, and confocal microscopy revealed microbial cells in aseptically regenerated leaves and roots. Clone libraries and tag-encoded FLX amplicon pyrosequencing (TEFAP) analysis amplified sequences from callus homologous to diverse fungal and bacterial taxa. Culturing isolated some seed borne endophyte taxa which could be readily propagated apart from the host. Microbial cells were observed within biofilm-like residues associated with plant cell surfaces and intercellular spaces. Various universal primers amplified both plant and microbial sequences, with different primers revealing different patterns of fungal diversity. Bacterial and fungal TEFAP followed by alignment with sequences from curated databases revealed 7 bacterial and 17 ascomycete taxa in A. canescens, and 5 bacterial taxa in A. torreyi. Additional diversity was observed among isolates and clone libraries. Micropropagated Atriplex retains a complex, intimately associated microbiome which includes diverse strains well poised to interact in manners that influence host physiology. Microbiome analysis was facilitated by high throughput sequencing methods, but primer biases continue to limit recovery of diverse sequences from even moderately complex communities.Mary E LuceroAdrian UncPeter CookeScot DowdShulei SunPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 3, p e17693 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mary E Lucero Adrian Unc Peter Cooke Scot Dowd Shulei Sun Endophyte microbiome diversity in micropropagated Atriplex canescens and Atriplex torreyi var griffithsii.
description	Microbial diversity associated with micropropagated Atriplex species was assessed using microscopy, isolate culturing, and sequencing. Light, electron, and confocal microscopy revealed microbial cells in aseptically regenerated leaves and roots. Clone libraries and tag-encoded FLX amplicon pyrosequencing (TEFAP) analysis amplified sequences from callus homologous to diverse fungal and bacterial taxa. Culturing isolated some seed borne endophyte taxa which could be readily propagated apart from the host. Microbial cells were observed within biofilm-like residues associated with plant cell surfaces and intercellular spaces. Various universal primers amplified both plant and microbial sequences, with different primers revealing different patterns of fungal diversity. Bacterial and fungal TEFAP followed by alignment with sequences from curated databases revealed 7 bacterial and 17 ascomycete taxa in A. canescens, and 5 bacterial taxa in A. torreyi. Additional diversity was observed among isolates and clone libraries. Micropropagated Atriplex retains a complex, intimately associated microbiome which includes diverse strains well poised to interact in manners that influence host physiology. Microbiome analysis was facilitated by high throughput sequencing methods, but primer biases continue to limit recovery of diverse sequences from even moderately complex communities.
format	article
author	Mary E Lucero Adrian Unc Peter Cooke Scot Dowd Shulei Sun
author_facet	Mary E Lucero Adrian Unc Peter Cooke Scot Dowd Shulei Sun
author_sort	Mary E Lucero
title	Endophyte microbiome diversity in micropropagated Atriplex canescens and Atriplex torreyi var griffithsii.
title_short	Endophyte microbiome diversity in micropropagated Atriplex canescens and Atriplex torreyi var griffithsii.
title_full	Endophyte microbiome diversity in micropropagated Atriplex canescens and Atriplex torreyi var griffithsii.
title_fullStr	Endophyte microbiome diversity in micropropagated Atriplex canescens and Atriplex torreyi var griffithsii.
title_full_unstemmed	Endophyte microbiome diversity in micropropagated Atriplex canescens and Atriplex torreyi var griffithsii.
title_sort	endophyte microbiome diversity in micropropagated atriplex canescens and atriplex torreyi var griffithsii.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/42a180c1478648508366e5096dccbd2e
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Endophyte microbiome diversity in micropropagated Atriplex canescens and Atriplex torreyi var griffithsii.

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