Decreased lung carcinoma cell density on select polymer nanometer surface features for lung replacement therapies

Lijuan Zhang1, Young Wook Chun2, Thomas J Webster21Department of Chemistry and 2Division of Engineering, Brown University, Providence, RI USAAbstract: Poly(lactic-co-glycolic) acid (PLGA) has been widely used as a biomaterial in regenerative medicine because of its biocompatibility and biodegradabil...

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Autores principales:	Lijuan Zhang, Young Wook Chun, Thomas J Webster
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/42a992d33dd7452186dddeae505ec519
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spelling	oai:doaj.org-article:42a992d33dd7452186dddeae505ec5192021-12-02T01:50:40ZDecreased lung carcinoma cell density on select polymer nanometer surface features for lung replacement therapies1176-91141178-2013https://doaj.org/article/42a992d33dd7452186dddeae505ec5192010-04-01T00:00:00Zhttp://www.dovepress.com/decreased-lung-carcinoma-cell-density-on-select-polymer-nanometer-surf-a4285https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Lijuan Zhang1, Young Wook Chun2, Thomas J Webster21Department of Chemistry and 2Division of Engineering, Brown University, Providence, RI USAAbstract: Poly(lactic-co-glycolic) acid (PLGA) has been widely used as a biomaterial in regenerative medicine because of its biocompatibility and biodegradability properties. Previous studies have shown that cells (such as bladder smooth muscle cells, chondrocytes, and osteoblasts) respond differently to nanostructured PLGA surfaces compared with nanosmooth surfaces. The purpose of the present in vitro research was to prepare PLGA films with various nanometer surface features and determine whether lung cancer epithelial cells respond differently to such topographies. To create nanosurface features on PLGA, different sized (190 nm, 300 nm, 400 nm, and 530 nm diameter) polystyrene beads were used to cast polydimethylsiloxane (PDMS) molds which were used as templates to create nanofeatured PLGA films. Atomic force microscopy (AFM) images and root mean square roughness (RMS) values indicated that the intended spherical surface nanotopographies on PLGA with RMS values of 2.23, 5.03, 5.42, and 36.90 nm were formed by employing 190, 300, 400, and 530 nm beads. A solution evaporation method was also utilized to modify PLGA surface features by using 8 wt% (to obtain an AFM RMS value of 0.62 nm) and 4 wt% (to obtain an AFM RMS value of 2.23 nm) PLGA in chloroform solutions. Most importantly, lung cancer epithelial cells adhered less on the PLGA surfaces with RMS values of 0.62, 2.23, and 5.42 nm after four hours of culture compared with any other PLGA surface created here. After three days, PLGA surfaces with an RMS value of 0.62 nm had much lower cell density than any other sample. In this manner, PLGA with specific nanometer surface features may inhibit lung cancer cell density which may provide an important biomaterial for the treatment of lung cancer (from drug delivery to regenerative medicine).Keywords: nanotechnology, cancer, nanotopography, lung carcinoma Lijuan ZhangYoung Wook ChunThomas J WebsterDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 269-275 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Lijuan Zhang Young Wook Chun Thomas J Webster Decreased lung carcinoma cell density on select polymer nanometer surface features for lung replacement therapies
description	Lijuan Zhang1, Young Wook Chun2, Thomas J Webster21Department of Chemistry and 2Division of Engineering, Brown University, Providence, RI USAAbstract: Poly(lactic-co-glycolic) acid (PLGA) has been widely used as a biomaterial in regenerative medicine because of its biocompatibility and biodegradability properties. Previous studies have shown that cells (such as bladder smooth muscle cells, chondrocytes, and osteoblasts) respond differently to nanostructured PLGA surfaces compared with nanosmooth surfaces. The purpose of the present in vitro research was to prepare PLGA films with various nanometer surface features and determine whether lung cancer epithelial cells respond differently to such topographies. To create nanosurface features on PLGA, different sized (190 nm, 300 nm, 400 nm, and 530 nm diameter) polystyrene beads were used to cast polydimethylsiloxane (PDMS) molds which were used as templates to create nanofeatured PLGA films. Atomic force microscopy (AFM) images and root mean square roughness (RMS) values indicated that the intended spherical surface nanotopographies on PLGA with RMS values of 2.23, 5.03, 5.42, and 36.90 nm were formed by employing 190, 300, 400, and 530 nm beads. A solution evaporation method was also utilized to modify PLGA surface features by using 8 wt% (to obtain an AFM RMS value of 0.62 nm) and 4 wt% (to obtain an AFM RMS value of 2.23 nm) PLGA in chloroform solutions. Most importantly, lung cancer epithelial cells adhered less on the PLGA surfaces with RMS values of 0.62, 2.23, and 5.42 nm after four hours of culture compared with any other PLGA surface created here. After three days, PLGA surfaces with an RMS value of 0.62 nm had much lower cell density than any other sample. In this manner, PLGA with specific nanometer surface features may inhibit lung cancer cell density which may provide an important biomaterial for the treatment of lung cancer (from drug delivery to regenerative medicine).Keywords: nanotechnology, cancer, nanotopography, lung carcinoma
format	article
author	Lijuan Zhang Young Wook Chun Thomas J Webster
author_facet	Lijuan Zhang Young Wook Chun Thomas J Webster
author_sort	Lijuan Zhang
title	Decreased lung carcinoma cell density on select polymer nanometer surface features for lung replacement therapies
title_short	Decreased lung carcinoma cell density on select polymer nanometer surface features for lung replacement therapies
title_full	Decreased lung carcinoma cell density on select polymer nanometer surface features for lung replacement therapies
title_fullStr	Decreased lung carcinoma cell density on select polymer nanometer surface features for lung replacement therapies
title_full_unstemmed	Decreased lung carcinoma cell density on select polymer nanometer surface features for lung replacement therapies
title_sort	decreased lung carcinoma cell density on select polymer nanometer surface features for lung replacement therapies
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/42a992d33dd7452186dddeae505ec519
work_keys_str_mv	AT lijuanzhang decreasedlungcarcinomacelldensityonselectpolymernanometersurfacefeaturesforlungreplacementtherapies AT youngwookchun decreasedlungcarcinomacelldensityonselectpolymernanometersurfacefeaturesforlungreplacementtherapies AT thomasjwebster decreasedlungcarcinomacelldensityonselectpolymernanometersurfacefeaturesforlungreplacementtherapies
_version_	1718402831346565120

Decreased lung carcinoma cell density on select polymer nanometer surface features for lung replacement therapies

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