Regulation of Virulence of <named-content content-type="genus-species">Entamoeba histolytica</named-content> by the URE3-BP Transcription Factor

ABSTRACT It is not understood why only some infections with Entamoeba histolytica result in disease. The calcium-regulated transcription factor upstream regulatory element 3-binding protein (URE3-BP) was initially identified by virtue of its role in regulating the expression of two amebic virulence...

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Autores principales:	Carol A. Gilchrist, Ellyn S. Moore, Yan Zhang, Christina B. Bousquet, Joanne A. Lannigan, Barbara J. Mann, William A. Petri
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2010
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/42aa3b0785f14072870f58eaccec80ed
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spelling	oai:doaj.org-article:42aa3b0785f14072870f58eaccec80ed2021-11-15T15:38:14ZRegulation of Virulence of <named-content content-type="genus-species">Entamoeba histolytica</named-content> by the URE3-BP Transcription Factor10.1128/mBio.00057-102150-7511https://doaj.org/article/42aa3b0785f14072870f58eaccec80ed2010-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00057-10https://doaj.org/toc/2150-7511ABSTRACT It is not understood why only some infections with Entamoeba histolytica result in disease. The calcium-regulated transcription factor upstream regulatory element 3-binding protein (URE3-BP) was initially identified by virtue of its role in regulating the expression of two amebic virulence genes, the Gal/GalNac lectin and ferredoxin. Here we tested whether this transcription factor has a broader role in regulating virulence. A comparison of in vivo to in vitro parasite gene expression demonstrated that 39% of in vivo regulated transcripts contained the URE3 motif recognized by URE3-BP, compared to 23% of all promoters (P < 0.0001). Amebae induced to express a dominant positive mutant form of URE3-BP had an increase in an elongated morphology (30% ± 6% versus 14% ± 5%; P = 0.001), a 2-fold competitive advantage at invading the intestinal epithelium (P = 0.017), and a 3-fold increase in liver abscess size (0.1 ± 0.1 g versus 0.036 ± 0.1 g; P = 0.03). These results support a role for URE3-BP in virulence regulation. IMPORTANCE Amebic dysentery and liver abscess are caused by Entamoeba histolytica. Amebae colonize the colon and cause disease by invading the intestinal epithelium. However, only one in five E. histolytica infections leads to disease. The factors that govern the transition from colonization to invasion are not understood. The transcription factor upstream regulatory element 3-binding protein (URE3-BP) is a calcium-responding regulator of the E. histolytica Gal/GalNAc lectin and ferredoxin genes, both implicated in virulence. Here we discovered that inducible expression of URE3-BP changed trophozoite morphology and promoted parasite invasion in the colon and liver. These results indicate that one determinant of virulence is transcriptional regulation by URE3-BP.Carol A. GilchristEllyn S. MooreYan ZhangChristina B. BousquetJoanne A. LanniganBarbara J. MannWilliam A. PetriAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 1, Iss 1 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Carol A. Gilchrist Ellyn S. Moore Yan Zhang Christina B. Bousquet Joanne A. Lannigan Barbara J. Mann William A. Petri Regulation of Virulence of <named-content content-type="genus-species">Entamoeba histolytica</named-content> by the URE3-BP Transcription Factor
description	ABSTRACT It is not understood why only some infections with Entamoeba histolytica result in disease. The calcium-regulated transcription factor upstream regulatory element 3-binding protein (URE3-BP) was initially identified by virtue of its role in regulating the expression of two amebic virulence genes, the Gal/GalNac lectin and ferredoxin. Here we tested whether this transcription factor has a broader role in regulating virulence. A comparison of in vivo to in vitro parasite gene expression demonstrated that 39% of in vivo regulated transcripts contained the URE3 motif recognized by URE3-BP, compared to 23% of all promoters (P < 0.0001). Amebae induced to express a dominant positive mutant form of URE3-BP had an increase in an elongated morphology (30% ± 6% versus 14% ± 5%; P = 0.001), a 2-fold competitive advantage at invading the intestinal epithelium (P = 0.017), and a 3-fold increase in liver abscess size (0.1 ± 0.1 g versus 0.036 ± 0.1 g; P = 0.03). These results support a role for URE3-BP in virulence regulation. IMPORTANCE Amebic dysentery and liver abscess are caused by Entamoeba histolytica. Amebae colonize the colon and cause disease by invading the intestinal epithelium. However, only one in five E. histolytica infections leads to disease. The factors that govern the transition from colonization to invasion are not understood. The transcription factor upstream regulatory element 3-binding protein (URE3-BP) is a calcium-responding regulator of the E. histolytica Gal/GalNAc lectin and ferredoxin genes, both implicated in virulence. Here we discovered that inducible expression of URE3-BP changed trophozoite morphology and promoted parasite invasion in the colon and liver. These results indicate that one determinant of virulence is transcriptional regulation by URE3-BP.
format	article
author	Carol A. Gilchrist Ellyn S. Moore Yan Zhang Christina B. Bousquet Joanne A. Lannigan Barbara J. Mann William A. Petri
author_facet	Carol A. Gilchrist Ellyn S. Moore Yan Zhang Christina B. Bousquet Joanne A. Lannigan Barbara J. Mann William A. Petri
author_sort	Carol A. Gilchrist
title	Regulation of Virulence of <named-content content-type="genus-species">Entamoeba histolytica</named-content> by the URE3-BP Transcription Factor
title_short	Regulation of Virulence of <named-content content-type="genus-species">Entamoeba histolytica</named-content> by the URE3-BP Transcription Factor
title_full	Regulation of Virulence of <named-content content-type="genus-species">Entamoeba histolytica</named-content> by the URE3-BP Transcription Factor
title_fullStr	Regulation of Virulence of <named-content content-type="genus-species">Entamoeba histolytica</named-content> by the URE3-BP Transcription Factor
title_full_unstemmed	Regulation of Virulence of <named-content content-type="genus-species">Entamoeba histolytica</named-content> by the URE3-BP Transcription Factor
title_sort	regulation of virulence of <named-content content-type="genus-species">entamoeba histolytica</named-content> by the ure3-bp transcription factor
publisher	American Society for Microbiology
publishDate	2010
url	https://doaj.org/article/42aa3b0785f14072870f58eaccec80ed
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Regulation of Virulence of <named-content content-type="genus-species">Entamoeba histolytica</named-content> by the URE3-BP Transcription Factor

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