Ethanol Alleviates Amyloid-β-Induced Toxicity in an Alzheimer’s Disease Model of Caenorhabiditis elegans

Amyloid-β, a hallmark of Alzheimer’s disease, forms toxic intracellular oligomers and extracellular senile plaques resulting in neuronal toxicity. Ethanol is widely consumed worldwide. Moderate ethanol consumption has numerous benefits in humans. We found that ethanol could significantly extend the...

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Autores principales:	Shuju Bai, Wenbo Wang, Zhiwei Zhang, Mengyao Li, Zehan Chen, Jiuqiao Wang, Yanlin Zhao, Lu An, Yuxiang Wang, Shu Xing, Xueqi Fu, Junfeng Ma
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Alzheimer’s disease amyloid-β Caenorhabiditis elegans ethanol DAF-16 lysosome Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/42ab69ae05c44253a7522b8e91b06038
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spelling	oai:doaj.org-article:42ab69ae05c44253a7522b8e91b060382021-11-12T05:16:57ZEthanol Alleviates Amyloid-β-Induced Toxicity in an Alzheimer’s Disease Model of Caenorhabiditis elegans1663-436510.3389/fnagi.2021.762659https://doaj.org/article/42ab69ae05c44253a7522b8e91b060382021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnagi.2021.762659/fullhttps://doaj.org/toc/1663-4365Amyloid-β, a hallmark of Alzheimer’s disease, forms toxic intracellular oligomers and extracellular senile plaques resulting in neuronal toxicity. Ethanol is widely consumed worldwide. Moderate ethanol consumption has numerous benefits in humans. We found that ethanol could significantly extend the lifespan of Caenorhabiditis elegans in a previous study. Based on that study, we tested the effect of ethanol on Alzheimer’s disease transgenic Caenorhabiditis elegans strain CL4176, which expresses amyloid-β1-42 peptide in body wall muscle cells. Ethanol delayed paralysis and reduced amyloid-β oligomers in Caenorhabiditis elegans worms of the CL4176 strain. Moreover, ethanol could induce the nuclear translocation of DAF-16 in the nematodes. However, in worms that were fed daf-16 RNAi bacteria, ethanol no longer delayed the paralysis. The qPCR assays showed that ethanol increases the expression of daf-16, hsf-1 and their common target genes- small heat shock protein genes. In addition, we also found that ethanol could increase lysosome mass in the CL4176 worms. In summary, our study indicated that ethanol attenuated amyloid-β toxicity in the Alzheimer’s disease model of Caenorhabiditis elegans via increasing the level of lysosomes to promote amyloid-β degradation and upregulating the levels of small heat shock protein genes to reduce amyloid-β aggregation.Shuju BaiWenbo WangZhiwei ZhangMengyao LiZehan ChenJiuqiao WangYanlin ZhaoLu AnYuxiang WangShu XingXueqi FuXueqi FuJunfeng MaJunfeng MaFrontiers Media S.A.articleAlzheimer’s diseaseamyloid-βCaenorhabiditis elegansethanolDAF-16lysosomeNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENFrontiers in Aging Neuroscience, Vol 13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Alzheimer’s disease amyloid-β Caenorhabiditis elegans ethanol DAF-16 lysosome Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	Alzheimer’s disease amyloid-β Caenorhabiditis elegans ethanol DAF-16 lysosome Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Shuju Bai Wenbo Wang Zhiwei Zhang Mengyao Li Zehan Chen Jiuqiao Wang Yanlin Zhao Lu An Yuxiang Wang Shu Xing Xueqi Fu Xueqi Fu Junfeng Ma Junfeng Ma Ethanol Alleviates Amyloid-β-Induced Toxicity in an Alzheimer’s Disease Model of Caenorhabiditis elegans
description	Amyloid-β, a hallmark of Alzheimer’s disease, forms toxic intracellular oligomers and extracellular senile plaques resulting in neuronal toxicity. Ethanol is widely consumed worldwide. Moderate ethanol consumption has numerous benefits in humans. We found that ethanol could significantly extend the lifespan of Caenorhabiditis elegans in a previous study. Based on that study, we tested the effect of ethanol on Alzheimer’s disease transgenic Caenorhabiditis elegans strain CL4176, which expresses amyloid-β1-42 peptide in body wall muscle cells. Ethanol delayed paralysis and reduced amyloid-β oligomers in Caenorhabiditis elegans worms of the CL4176 strain. Moreover, ethanol could induce the nuclear translocation of DAF-16 in the nematodes. However, in worms that were fed daf-16 RNAi bacteria, ethanol no longer delayed the paralysis. The qPCR assays showed that ethanol increases the expression of daf-16, hsf-1 and their common target genes- small heat shock protein genes. In addition, we also found that ethanol could increase lysosome mass in the CL4176 worms. In summary, our study indicated that ethanol attenuated amyloid-β toxicity in the Alzheimer’s disease model of Caenorhabiditis elegans via increasing the level of lysosomes to promote amyloid-β degradation and upregulating the levels of small heat shock protein genes to reduce amyloid-β aggregation.
format	article
author	Shuju Bai Wenbo Wang Zhiwei Zhang Mengyao Li Zehan Chen Jiuqiao Wang Yanlin Zhao Lu An Yuxiang Wang Shu Xing Xueqi Fu Xueqi Fu Junfeng Ma Junfeng Ma
author_facet	Shuju Bai Wenbo Wang Zhiwei Zhang Mengyao Li Zehan Chen Jiuqiao Wang Yanlin Zhao Lu An Yuxiang Wang Shu Xing Xueqi Fu Xueqi Fu Junfeng Ma Junfeng Ma
author_sort	Shuju Bai
title	Ethanol Alleviates Amyloid-β-Induced Toxicity in an Alzheimer’s Disease Model of Caenorhabiditis elegans
title_short	Ethanol Alleviates Amyloid-β-Induced Toxicity in an Alzheimer’s Disease Model of Caenorhabiditis elegans
title_full	Ethanol Alleviates Amyloid-β-Induced Toxicity in an Alzheimer’s Disease Model of Caenorhabiditis elegans
title_fullStr	Ethanol Alleviates Amyloid-β-Induced Toxicity in an Alzheimer’s Disease Model of Caenorhabiditis elegans
title_full_unstemmed	Ethanol Alleviates Amyloid-β-Induced Toxicity in an Alzheimer’s Disease Model of Caenorhabiditis elegans
title_sort	ethanol alleviates amyloid-β-induced toxicity in an alzheimer’s disease model of caenorhabiditis elegans
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/42ab69ae05c44253a7522b8e91b06038
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Ethanol Alleviates Amyloid-β-Induced Toxicity in an Alzheimer’s Disease Model of Caenorhabiditis elegans

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