Transcriptomic profiling of Gh/Igf system reveals a prompted tissue-specific differentiation and novel hypoxia responsive genes in gilthead sea bream

Abstract A customized PCR-array was used for the simultaneous gene expression of the Gh/Igf system and related markers of muscle growth, and lipid and energy metabolism during early life stages of gilthead sea bream (60–127 days posthatching). Also, transcriptional reprogramming by mild hypoxia was...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	F. Naya-Català, P. Simó-Mirabet, J. Calduch-Giner, J. Pérez-Sánchez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/42b4522442474f5eb5cd7e57bde962bc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:42b4522442474f5eb5cd7e57bde962bc
record_format	dspace
spelling	oai:doaj.org-article:42b4522442474f5eb5cd7e57bde962bc2021-12-02T15:08:10ZTranscriptomic profiling of Gh/Igf system reveals a prompted tissue-specific differentiation and novel hypoxia responsive genes in gilthead sea bream10.1038/s41598-021-95408-62045-2322https://doaj.org/article/42b4522442474f5eb5cd7e57bde962bc2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95408-6https://doaj.org/toc/2045-2322Abstract A customized PCR-array was used for the simultaneous gene expression of the Gh/Igf system and related markers of muscle growth, and lipid and energy metabolism during early life stages of gilthead sea bream (60–127 days posthatching). Also, transcriptional reprogramming by mild hypoxia was assessed in fingerling fish with different history trajectories on O2 availability during the same time window. In normoxic fish, the expression of almost all the genes in the array varied over time with a prompted liver and muscle tissue-specific differentiation, which also revealed temporal changes in the relative expression of markers of the full gilthead sea bream repertoire of Gh receptors, Igfs and Igf-binding proteins. Results supported a different contribution through development of ghr and igf subtypes on the type of action of GH via systemic or direct effects at the local tissue level. This was extensive to Igfbp1/2/4 and Igfbp3/5/6 clades that clearly evolved through development as hepatic and muscle Igfbp subtypes, respectively. This trade-off is however very plastic to cope changes in the environment, and ghr1 and igfbp1/3/4/5 emerged as hypoxic imprinting genes during critical early developmental windows leading to recognize individuals with different history trajectories of oxygen availability and metabolic capabilities later in life.F. Naya-CatalàP. Simó-MirabetJ. Calduch-GinerJ. Pérez-SánchezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q F. Naya-Català P. Simó-Mirabet J. Calduch-Giner J. Pérez-Sánchez Transcriptomic profiling of Gh/Igf system reveals a prompted tissue-specific differentiation and novel hypoxia responsive genes in gilthead sea bream
description	Abstract A customized PCR-array was used for the simultaneous gene expression of the Gh/Igf system and related markers of muscle growth, and lipid and energy metabolism during early life stages of gilthead sea bream (60–127 days posthatching). Also, transcriptional reprogramming by mild hypoxia was assessed in fingerling fish with different history trajectories on O2 availability during the same time window. In normoxic fish, the expression of almost all the genes in the array varied over time with a prompted liver and muscle tissue-specific differentiation, which also revealed temporal changes in the relative expression of markers of the full gilthead sea bream repertoire of Gh receptors, Igfs and Igf-binding proteins. Results supported a different contribution through development of ghr and igf subtypes on the type of action of GH via systemic or direct effects at the local tissue level. This was extensive to Igfbp1/2/4 and Igfbp3/5/6 clades that clearly evolved through development as hepatic and muscle Igfbp subtypes, respectively. This trade-off is however very plastic to cope changes in the environment, and ghr1 and igfbp1/3/4/5 emerged as hypoxic imprinting genes during critical early developmental windows leading to recognize individuals with different history trajectories of oxygen availability and metabolic capabilities later in life.
format	article
author	F. Naya-Català P. Simó-Mirabet J. Calduch-Giner J. Pérez-Sánchez
author_facet	F. Naya-Català P. Simó-Mirabet J. Calduch-Giner J. Pérez-Sánchez
author_sort	F. Naya-Català
title	Transcriptomic profiling of Gh/Igf system reveals a prompted tissue-specific differentiation and novel hypoxia responsive genes in gilthead sea bream
title_short	Transcriptomic profiling of Gh/Igf system reveals a prompted tissue-specific differentiation and novel hypoxia responsive genes in gilthead sea bream
title_full	Transcriptomic profiling of Gh/Igf system reveals a prompted tissue-specific differentiation and novel hypoxia responsive genes in gilthead sea bream
title_fullStr	Transcriptomic profiling of Gh/Igf system reveals a prompted tissue-specific differentiation and novel hypoxia responsive genes in gilthead sea bream
title_full_unstemmed	Transcriptomic profiling of Gh/Igf system reveals a prompted tissue-specific differentiation and novel hypoxia responsive genes in gilthead sea bream
title_sort	transcriptomic profiling of gh/igf system reveals a prompted tissue-specific differentiation and novel hypoxia responsive genes in gilthead sea bream
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/42b4522442474f5eb5cd7e57bde962bc
work_keys_str_mv	AT fnayacatala transcriptomicprofilingofghigfsystemrevealsapromptedtissuespecificdifferentiationandnovelhypoxiaresponsivegenesingiltheadseabream AT psimomirabet transcriptomicprofilingofghigfsystemrevealsapromptedtissuespecificdifferentiationandnovelhypoxiaresponsivegenesingiltheadseabream AT jcalduchginer transcriptomicprofilingofghigfsystemrevealsapromptedtissuespecificdifferentiationandnovelhypoxiaresponsivegenesingiltheadseabream AT jperezsanchez transcriptomicprofilingofghigfsystemrevealsapromptedtissuespecificdifferentiationandnovelhypoxiaresponsivegenesingiltheadseabream
_version_	1718388247423352832

Transcriptomic profiling of Gh/Igf system reveals a prompted tissue-specific differentiation and novel hypoxia responsive genes in gilthead sea bream

Ejemplares similares