Genome-Wide Scanning of Potential Hotspots for Adenosine Methylation: A Potential Path to Neuronal Development

Methylation of adenosines at N6 position (m6A) is the most frequent internal modification in mRNAs of the human genome and attributable to diverse roles in physiological development, and pathophysiological processes. However, studies on the role of m6A in neuronal development are sparse and not well...

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Auteurs principaux:	Sanjay Kumar, Lung-Wen Tsai, Pavan Kumar, Rajni Dubey, Deepika Gupta, Anjani Kumar Singh, Vishnu Swarup, Himanshu Narayan Singh
Format:	article
Langue:	EN
Publié:	MDPI AG 2021
Sujets:	adenosine methylation m6A RNA modification neuronal development Science Q
Accès en ligne:	https://doaj.org/article/42bcb81b50ec4d8083b55d9fd41ff6f3
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spelling	oai:doaj.org-article:42bcb81b50ec4d8083b55d9fd41ff6f32021-11-25T18:10:59ZGenome-Wide Scanning of Potential Hotspots for Adenosine Methylation: A Potential Path to Neuronal Development10.3390/life111111852075-1729https://doaj.org/article/42bcb81b50ec4d8083b55d9fd41ff6f32021-11-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1185https://doaj.org/toc/2075-1729Methylation of adenosines at N6 position (m6A) is the most frequent internal modification in mRNAs of the human genome and attributable to diverse roles in physiological development, and pathophysiological processes. However, studies on the role of m6A in neuronal development are sparse and not well-documented. The m6A detection remains challenging due to its inconsistent pattern and less sensitivity by the current detection techniques. Therefore, we applied a sliding window technique to identify the consensus site (5′-GGACT-3′) <i>n</i> ≥ 2 and annotated all m6A hotspots in the human genome. Over 6.78 × 10<sup>7</sup> hotspots were identified and 96.4% were found to be located in the non-coding regions, suggesting that methylation occurs before splicing. Several genes, <i>RPS6K, NRP1, NRXN, EGFR, YTHDF2</i>, have been involved in various stages of neuron development and their functioning. However, the contribution of m6A in these genes needs further validation in the experimental model. Thus, the present study elaborates the location of m6A in the human genome and its function in neuron physiology.Sanjay KumarLung-Wen TsaiPavan KumarRajni DubeyDeepika GuptaAnjani Kumar SinghVishnu SwarupHimanshu Narayan SinghMDPI AGarticleadenosine methylationm6ARNA modificationneuronal developmentScienceQENLife, Vol 11, Iss 1185, p 1185 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adenosine methylation m6A RNA modification neuronal development Science Q
spellingShingle	adenosine methylation m6A RNA modification neuronal development Science Q Sanjay Kumar Lung-Wen Tsai Pavan Kumar Rajni Dubey Deepika Gupta Anjani Kumar Singh Vishnu Swarup Himanshu Narayan Singh Genome-Wide Scanning of Potential Hotspots for Adenosine Methylation: A Potential Path to Neuronal Development
description	Methylation of adenosines at N6 position (m6A) is the most frequent internal modification in mRNAs of the human genome and attributable to diverse roles in physiological development, and pathophysiological processes. However, studies on the role of m6A in neuronal development are sparse and not well-documented. The m6A detection remains challenging due to its inconsistent pattern and less sensitivity by the current detection techniques. Therefore, we applied a sliding window technique to identify the consensus site (5′-GGACT-3′) <i>n</i> ≥ 2 and annotated all m6A hotspots in the human genome. Over 6.78 × 10<sup>7</sup> hotspots were identified and 96.4% were found to be located in the non-coding regions, suggesting that methylation occurs before splicing. Several genes, <i>RPS6K, NRP1, NRXN, EGFR, YTHDF2</i>, have been involved in various stages of neuron development and their functioning. However, the contribution of m6A in these genes needs further validation in the experimental model. Thus, the present study elaborates the location of m6A in the human genome and its function in neuron physiology.
format	article
author	Sanjay Kumar Lung-Wen Tsai Pavan Kumar Rajni Dubey Deepika Gupta Anjani Kumar Singh Vishnu Swarup Himanshu Narayan Singh
author_facet	Sanjay Kumar Lung-Wen Tsai Pavan Kumar Rajni Dubey Deepika Gupta Anjani Kumar Singh Vishnu Swarup Himanshu Narayan Singh
author_sort	Sanjay Kumar
title	Genome-Wide Scanning of Potential Hotspots for Adenosine Methylation: A Potential Path to Neuronal Development
title_short	Genome-Wide Scanning of Potential Hotspots for Adenosine Methylation: A Potential Path to Neuronal Development
title_full	Genome-Wide Scanning of Potential Hotspots for Adenosine Methylation: A Potential Path to Neuronal Development
title_fullStr	Genome-Wide Scanning of Potential Hotspots for Adenosine Methylation: A Potential Path to Neuronal Development
title_full_unstemmed	Genome-Wide Scanning of Potential Hotspots for Adenosine Methylation: A Potential Path to Neuronal Development
title_sort	genome-wide scanning of potential hotspots for adenosine methylation: a potential path to neuronal development
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/42bcb81b50ec4d8083b55d9fd41ff6f3
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Genome-Wide Scanning of Potential Hotspots for Adenosine Methylation: A Potential Path to Neuronal Development

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