The velvet family of fungal regulators contains a DNA-binding domain structurally similar to NF-κB.
Morphological development of fungi and their combined production of secondary metabolites are both acting in defence and protection. These processes are mainly coordinated by velvet regulators, which contain a yet functionally and structurally uncharacterized velvet domain. Here we demonstrate that...
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Public Library of Science (PLoS)
2013
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oai:doaj.org-article:42c5c30ca5314eb29550a7485a1cacd32021-11-18T05:37:40ZThe velvet family of fungal regulators contains a DNA-binding domain structurally similar to NF-κB.1544-91731545-788510.1371/journal.pbio.1001750https://doaj.org/article/42c5c30ca5314eb29550a7485a1cacd32013-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24391470/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Morphological development of fungi and their combined production of secondary metabolites are both acting in defence and protection. These processes are mainly coordinated by velvet regulators, which contain a yet functionally and structurally uncharacterized velvet domain. Here we demonstrate that the velvet domain of VosA is a novel DNA-binding motif that specifically recognizes an 11-nucleotide consensus sequence consisting of two motifs in the promoters of key developmental regulatory genes. The crystal structure analysis of the VosA velvet domain revealed an unforeseen structural similarity with the Rel homology domain (RHD) of the mammalian transcription factor NF-κB. Based on this structural similarity several conserved amino acid residues present in all velvet domains have been identified and shown to be essential for the DNA binding ability of VosA. The velvet domain is also involved in dimer formation as seen in the solved crystal structures of the VosA homodimer and the VosA-VelB heterodimer. These findings suggest that defence mechanisms of both fungi and animals might be governed by structurally related DNA-binding transcription factors.Yasar Luqman AhmedJennifer GerkeHee-Soo ParkÖzgür BayramPiotr NeumannMin NiAchim DickmannsSun Chang KimJae-Hyuk YuGerhard H BrausRalf FicnerPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 11, Iss 12, p e1001750 (2013) |
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DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Biology (General) QH301-705.5 |
| spellingShingle |
Biology (General) QH301-705.5 Yasar Luqman Ahmed Jennifer Gerke Hee-Soo Park Özgür Bayram Piotr Neumann Min Ni Achim Dickmanns Sun Chang Kim Jae-Hyuk Yu Gerhard H Braus Ralf Ficner The velvet family of fungal regulators contains a DNA-binding domain structurally similar to NF-κB. |
| description |
Morphological development of fungi and their combined production of secondary metabolites are both acting in defence and protection. These processes are mainly coordinated by velvet regulators, which contain a yet functionally and structurally uncharacterized velvet domain. Here we demonstrate that the velvet domain of VosA is a novel DNA-binding motif that specifically recognizes an 11-nucleotide consensus sequence consisting of two motifs in the promoters of key developmental regulatory genes. The crystal structure analysis of the VosA velvet domain revealed an unforeseen structural similarity with the Rel homology domain (RHD) of the mammalian transcription factor NF-κB. Based on this structural similarity several conserved amino acid residues present in all velvet domains have been identified and shown to be essential for the DNA binding ability of VosA. The velvet domain is also involved in dimer formation as seen in the solved crystal structures of the VosA homodimer and the VosA-VelB heterodimer. These findings suggest that defence mechanisms of both fungi and animals might be governed by structurally related DNA-binding transcription factors. |
| format |
article |
| author |
Yasar Luqman Ahmed Jennifer Gerke Hee-Soo Park Özgür Bayram Piotr Neumann Min Ni Achim Dickmanns Sun Chang Kim Jae-Hyuk Yu Gerhard H Braus Ralf Ficner |
| author_facet |
Yasar Luqman Ahmed Jennifer Gerke Hee-Soo Park Özgür Bayram Piotr Neumann Min Ni Achim Dickmanns Sun Chang Kim Jae-Hyuk Yu Gerhard H Braus Ralf Ficner |
| author_sort |
Yasar Luqman Ahmed |
| title |
The velvet family of fungal regulators contains a DNA-binding domain structurally similar to NF-κB. |
| title_short |
The velvet family of fungal regulators contains a DNA-binding domain structurally similar to NF-κB. |
| title_full |
The velvet family of fungal regulators contains a DNA-binding domain structurally similar to NF-κB. |
| title_fullStr |
The velvet family of fungal regulators contains a DNA-binding domain structurally similar to NF-κB. |
| title_full_unstemmed |
The velvet family of fungal regulators contains a DNA-binding domain structurally similar to NF-κB. |
| title_sort |
velvet family of fungal regulators contains a dna-binding domain structurally similar to nf-κb. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/42c5c30ca5314eb29550a7485a1cacd3 |
| work_keys_str_mv |
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| _version_ |
1718424845184663552 |