The HOG pathway is critical for the colonization of the mouse gastrointestinal tract by Candida albicans.

The opportunistic pathogen Candida albicans is a frequent inhabitant of the human gastrointestinal tract where it usually behaves as a harmless commensal. In this particular niche, it needs to adapt to the different micro environments that challenge its survival within the host. In order to determin...

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Autores principales:	Daniel Prieto, Elvira Román, Inês Correia, Jesus Pla
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/42c6848bca174ca6b63260e1fb1ba675
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spelling	oai:doaj.org-article:42c6848bca174ca6b63260e1fb1ba6752021-11-18T08:35:35ZThe HOG pathway is critical for the colonization of the mouse gastrointestinal tract by Candida albicans.1932-620310.1371/journal.pone.0087128https://doaj.org/article/42c6848bca174ca6b63260e1fb1ba6752014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24475243/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The opportunistic pathogen Candida albicans is a frequent inhabitant of the human gastrointestinal tract where it usually behaves as a harmless commensal. In this particular niche, it needs to adapt to the different micro environments that challenge its survival within the host. In order to determine those factors involved in gut adaptation, we have used a gastrointestinal model of colonization in mouse to trace the behaviour of fungal cells. We have developed a genetic labelling system based on the complementary spectral properties of the fluorescent proteins GFP and a new C. albicans codon-adapted RFP (dTOM2) that allow a precise quantification of the fungal population in the gut via standard in vitro cultures or flow cytometry. This methodology has allowed us to determine the role of the three MAP kinase pathways of C. albicans (mediated by the MAPK Mkc1, Cek1 or Hog1) in mouse gut colonization via competitive assays with MAPK pathway mutants and their isogenic wild type strain. This approach reveals the signalling through HOG pathway as a critical factor influencing the establishment of C. albicans in the mouse gut. Less pronounced effects for mkc1 or cek1 mutants were found, only evident after 2-3 weeks of colonization. We have also seen that hog1 mutants is defective in adhesion to the gut mucosa and sensitive to bile salts. Finally, we have developed a genetic strategy for the in vivo excision (tetracycline-dependent) of any specific gene during the course of colonization in this particular niche, allowing the analysis of its role during gut colonization.Daniel PrietoElvira RománInês CorreiaJesus PlaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e87128 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniel Prieto Elvira Román Inês Correia Jesus Pla The HOG pathway is critical for the colonization of the mouse gastrointestinal tract by Candida albicans.
description	The opportunistic pathogen Candida albicans is a frequent inhabitant of the human gastrointestinal tract where it usually behaves as a harmless commensal. In this particular niche, it needs to adapt to the different micro environments that challenge its survival within the host. In order to determine those factors involved in gut adaptation, we have used a gastrointestinal model of colonization in mouse to trace the behaviour of fungal cells. We have developed a genetic labelling system based on the complementary spectral properties of the fluorescent proteins GFP and a new C. albicans codon-adapted RFP (dTOM2) that allow a precise quantification of the fungal population in the gut via standard in vitro cultures or flow cytometry. This methodology has allowed us to determine the role of the three MAP kinase pathways of C. albicans (mediated by the MAPK Mkc1, Cek1 or Hog1) in mouse gut colonization via competitive assays with MAPK pathway mutants and their isogenic wild type strain. This approach reveals the signalling through HOG pathway as a critical factor influencing the establishment of C. albicans in the mouse gut. Less pronounced effects for mkc1 or cek1 mutants were found, only evident after 2-3 weeks of colonization. We have also seen that hog1 mutants is defective in adhesion to the gut mucosa and sensitive to bile salts. Finally, we have developed a genetic strategy for the in vivo excision (tetracycline-dependent) of any specific gene during the course of colonization in this particular niche, allowing the analysis of its role during gut colonization.
format	article
author	Daniel Prieto Elvira Román Inês Correia Jesus Pla
author_facet	Daniel Prieto Elvira Román Inês Correia Jesus Pla
author_sort	Daniel Prieto
title	The HOG pathway is critical for the colonization of the mouse gastrointestinal tract by Candida albicans.
title_short	The HOG pathway is critical for the colonization of the mouse gastrointestinal tract by Candida albicans.
title_full	The HOG pathway is critical for the colonization of the mouse gastrointestinal tract by Candida albicans.
title_fullStr	The HOG pathway is critical for the colonization of the mouse gastrointestinal tract by Candida albicans.
title_full_unstemmed	The HOG pathway is critical for the colonization of the mouse gastrointestinal tract by Candida albicans.
title_sort	hog pathway is critical for the colonization of the mouse gastrointestinal tract by candida albicans.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/42c6848bca174ca6b63260e1fb1ba675
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The HOG pathway is critical for the colonization of the mouse gastrointestinal tract by Candida albicans.

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