Persistent hyperammonia and altered concentrations of urea cycle metabolites in a 5-day swine experiment of sepsis

Abstract We measured plasma and cerebrospinal fluid (CSF) metabolite concentrations in a 5-day porcine sepsis model of fecal peritonitis. The objectives were: (i) to verify whether the expected pathways that had emerged in previous studies pertain only to the early inflammatory response or persist f...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Manuela Ferrario, Roberta Pastorelli, Laura Brunelli, Shengchen Liu, Pedro Paulo Zanella do Amaral Campos, Daniela Casoni, Werner J. Z’Graggen, Stephan M. Jakob
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/42c83fff2d0f45b7b5c0834643ddebe8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:42c83fff2d0f45b7b5c0834643ddebe8
record_format	dspace
spelling	oai:doaj.org-article:42c83fff2d0f45b7b5c0834643ddebe82021-12-02T18:02:22ZPersistent hyperammonia and altered concentrations of urea cycle metabolites in a 5-day swine experiment of sepsis10.1038/s41598-021-97855-72045-2322https://doaj.org/article/42c83fff2d0f45b7b5c0834643ddebe82021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97855-7https://doaj.org/toc/2045-2322Abstract We measured plasma and cerebrospinal fluid (CSF) metabolite concentrations in a 5-day porcine sepsis model of fecal peritonitis. The objectives were: (i) to verify whether the expected pathways that had emerged in previous studies pertain only to the early inflammatory response or persist for the subsequent days; (ii) to identify metabolic derangements that arise later; (iii) to verify whether CSF metabolite concentrations were altered and if these alterations were similar to those in the blood or delayed. We observed an early response to inflammation and cytokine storms with alterations in lipid and glucose metabolism. The arginine/asymmetric dimethylarginine (ADMA) and phenylalanine/tyrosine balances changed 24 h after resuscitation in plasma, and later in CSF. There was a rise in ammonia concentration, with altered concentrations of metabolites in the urea cycle. Whether persistent derangement of these pathways have a role not only on short-term outcomes but also on longer-term comorbidities, such as septic encephalopathy, should be addressed in further studies.Manuela FerrarioRoberta PastorelliLaura BrunelliShengchen LiuPedro Paulo Zanella do Amaral CamposDaniela CasoniWerner J. Z’GraggenStephan M. JakobNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Manuela Ferrario Roberta Pastorelli Laura Brunelli Shengchen Liu Pedro Paulo Zanella do Amaral Campos Daniela Casoni Werner J. Z’Graggen Stephan M. Jakob Persistent hyperammonia and altered concentrations of urea cycle metabolites in a 5-day swine experiment of sepsis
description	Abstract We measured plasma and cerebrospinal fluid (CSF) metabolite concentrations in a 5-day porcine sepsis model of fecal peritonitis. The objectives were: (i) to verify whether the expected pathways that had emerged in previous studies pertain only to the early inflammatory response or persist for the subsequent days; (ii) to identify metabolic derangements that arise later; (iii) to verify whether CSF metabolite concentrations were altered and if these alterations were similar to those in the blood or delayed. We observed an early response to inflammation and cytokine storms with alterations in lipid and glucose metabolism. The arginine/asymmetric dimethylarginine (ADMA) and phenylalanine/tyrosine balances changed 24 h after resuscitation in plasma, and later in CSF. There was a rise in ammonia concentration, with altered concentrations of metabolites in the urea cycle. Whether persistent derangement of these pathways have a role not only on short-term outcomes but also on longer-term comorbidities, such as septic encephalopathy, should be addressed in further studies.
format	article
author	Manuela Ferrario Roberta Pastorelli Laura Brunelli Shengchen Liu Pedro Paulo Zanella do Amaral Campos Daniela Casoni Werner J. Z’Graggen Stephan M. Jakob
author_facet	Manuela Ferrario Roberta Pastorelli Laura Brunelli Shengchen Liu Pedro Paulo Zanella do Amaral Campos Daniela Casoni Werner J. Z’Graggen Stephan M. Jakob
author_sort	Manuela Ferrario
title	Persistent hyperammonia and altered concentrations of urea cycle metabolites in a 5-day swine experiment of sepsis
title_short	Persistent hyperammonia and altered concentrations of urea cycle metabolites in a 5-day swine experiment of sepsis
title_full	Persistent hyperammonia and altered concentrations of urea cycle metabolites in a 5-day swine experiment of sepsis
title_fullStr	Persistent hyperammonia and altered concentrations of urea cycle metabolites in a 5-day swine experiment of sepsis
title_full_unstemmed	Persistent hyperammonia and altered concentrations of urea cycle metabolites in a 5-day swine experiment of sepsis
title_sort	persistent hyperammonia and altered concentrations of urea cycle metabolites in a 5-day swine experiment of sepsis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/42c83fff2d0f45b7b5c0834643ddebe8
work_keys_str_mv	AT manuelaferrario persistenthyperammoniaandalteredconcentrationsofureacyclemetabolitesina5dayswineexperimentofsepsis AT robertapastorelli persistenthyperammoniaandalteredconcentrationsofureacyclemetabolitesina5dayswineexperimentofsepsis AT laurabrunelli persistenthyperammoniaandalteredconcentrationsofureacyclemetabolitesina5dayswineexperimentofsepsis AT shengchenliu persistenthyperammoniaandalteredconcentrationsofureacyclemetabolitesina5dayswineexperimentofsepsis AT pedropaulozanelladoamaralcampos persistenthyperammoniaandalteredconcentrationsofureacyclemetabolitesina5dayswineexperimentofsepsis AT danielacasoni persistenthyperammoniaandalteredconcentrationsofureacyclemetabolitesina5dayswineexperimentofsepsis AT wernerjzgraggen persistenthyperammoniaandalteredconcentrationsofureacyclemetabolitesina5dayswineexperimentofsepsis AT stephanmjakob persistenthyperammoniaandalteredconcentrationsofureacyclemetabolitesina5dayswineexperimentofsepsis
_version_	1718378922628874240

Persistent hyperammonia and altered concentrations of urea cycle metabolites in a 5-day swine experiment of sepsis

Ejemplares similares