Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi

Abstract Myzus persicae is a major pest of many crops including canola and Brassica vegetables, partly because it vectors plant viruses. Previously it has been reported that double-stranded RNA delivered to aphids by injection, artificial diet or transgenic plants has knocked down target genes and c...

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Autores principales:	Amol Bharat Ghodke, Robert Trygve Good, John F. Golz, Derek A. Russell, Owain Edwards, Charles Robin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/42ccc7d6cc9f4614ab09b7d22c64a220
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spelling	oai:doaj.org-article:42ccc7d6cc9f4614ab09b7d22c64a2202021-12-02T15:09:41ZExtracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi10.1038/s41598-019-47357-42045-2322https://doaj.org/article/42ccc7d6cc9f4614ab09b7d22c64a2202019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-47357-4https://doaj.org/toc/2045-2322Abstract Myzus persicae is a major pest of many crops including canola and Brassica vegetables, partly because it vectors plant viruses. Previously it has been reported that double-stranded RNA delivered to aphids by injection, artificial diet or transgenic plants has knocked down target genes and caused phenotypic effects. While these studies suggest that RNA interference (RNAi) might be used to suppress aphid populations, none have shown effects sufficient for field control. The current study analyses the efficacy of dsRNA directed against previously reported gene-targets on Green peach aphid (Myzus persicae) strains. No silencing effect was observed when dsRNA was delivered in artificial diet with or without transfection reagents. dsRNA produced in planta also failed to induce significant RNAi in M. persicae. Transcriptome analyses of the midgut suggested other potential targets including the Ferritin heavy chain transcripts, but they also could not be knocked down with dsRNA. Here we show that dsRNA is rapidly degraded by midgut secretions of Myzus persicae. Analysis of the transcriptome of the M. persicae midgut revealed that an ortholog of RNases from other insects was abundant.Amol Bharat GhodkeRobert Trygve GoodJohn F. GolzDerek A. RussellOwain EdwardsCharles RobinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-14 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amol Bharat Ghodke Robert Trygve Good John F. Golz Derek A. Russell Owain Edwards Charles Robin Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi
description	Abstract Myzus persicae is a major pest of many crops including canola and Brassica vegetables, partly because it vectors plant viruses. Previously it has been reported that double-stranded RNA delivered to aphids by injection, artificial diet or transgenic plants has knocked down target genes and caused phenotypic effects. While these studies suggest that RNA interference (RNAi) might be used to suppress aphid populations, none have shown effects sufficient for field control. The current study analyses the efficacy of dsRNA directed against previously reported gene-targets on Green peach aphid (Myzus persicae) strains. No silencing effect was observed when dsRNA was delivered in artificial diet with or without transfection reagents. dsRNA produced in planta also failed to induce significant RNAi in M. persicae. Transcriptome analyses of the midgut suggested other potential targets including the Ferritin heavy chain transcripts, but they also could not be knocked down with dsRNA. Here we show that dsRNA is rapidly degraded by midgut secretions of Myzus persicae. Analysis of the transcriptome of the M. persicae midgut revealed that an ortholog of RNases from other insects was abundant.
format	article
author	Amol Bharat Ghodke Robert Trygve Good John F. Golz Derek A. Russell Owain Edwards Charles Robin
author_facet	Amol Bharat Ghodke Robert Trygve Good John F. Golz Derek A. Russell Owain Edwards Charles Robin
author_sort	Amol Bharat Ghodke
title	Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi
title_short	Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi
title_full	Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi
title_fullStr	Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi
title_full_unstemmed	Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi
title_sort	extracellular endonucleases in the midgut of myzus persicae may limit the efficacy of orally delivered rnai
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/42ccc7d6cc9f4614ab09b7d22c64a220
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Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi

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