Screening and cellular validation of prognostic genes regulated by super enhancers in oral squamous cell carcinoma

Oral squamous cell carcinoma (OSCC) is the leading cause of death in patients with head and neck cancer. Reliable biomarkers to guide treatment decisions for OSCC remain scarce. The purpose of this study was to identify novel prognostic markers regulated by super enhancers in OSCC. Eight modules wer...

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Autores principales:	Liru zhang, Huanju li, Yongle qiu, Yuanhang liu, Xin liu, Wenjing wang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	oral squamous cell carcinoma super enhancer prognosis weighted gene co-expression network analysis Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/42ce5f144bfb494da194330b1863e2fc
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spelling	oai:doaj.org-article:42ce5f144bfb494da194330b1863e2fc2021-11-04T15:51:54ZScreening and cellular validation of prognostic genes regulated by super enhancers in oral squamous cell carcinoma2165-59792165-598710.1080/21655979.2021.1997089https://doaj.org/article/42ce5f144bfb494da194330b1863e2fc2021-10-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1997089https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Oral squamous cell carcinoma (OSCC) is the leading cause of death in patients with head and neck cancer. Reliable biomarkers to guide treatment decisions for OSCC remain scarce. The purpose of this study was to identify novel prognostic markers regulated by super enhancers in OSCC. Eight modules were obtained by weighted gene co-expression network analysis (WGCNA), among which MEblue module had the highest correlation with tumor stage, alcohol consumption and smoking. There were 41 genes regulated by super enhancers in MEblue module. Functional analysis showed that 41 super enhancer-regulated genes were involved in cancer progression. A total of twenty transcription factors of the 41 genes were predicted. Prognostic analysis of the 41 genes and the top 5 transcription factors showed that patients with high expression of AHCY, KCMF1, MANBAL and TFDP1 had a poor prognosis. Immunohistochemical analysis showed that AHCY, KCMF1 and MANBAL were highly expressed in OSCC tissue. Cellular experiment demonstrated that TFDP1 promoted AHCY, KCMF1 and MANBAL expression by binding to the super enhancers of these genes. Knockdown of TFDP1, AHCY, KCMF1 and MANBAL inhibited the proliferation of OSCC cells. In conclusion, AHCY, KCMF1 and MANBAL were recognized as super enhancer-regulated prognostic biomarkers regulated by TFDP1 in OSCC.Liru zhangHuanju liYongle qiuYuanhang liuXin liuWenjing wangTaylor & Francis Grouparticleoral squamous cell carcinomasuper enhancerprognosisweighted gene co-expression network analysisBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	oral squamous cell carcinoma super enhancer prognosis weighted gene co-expression network analysis Biotechnology TP248.13-248.65
spellingShingle	oral squamous cell carcinoma super enhancer prognosis weighted gene co-expression network analysis Biotechnology TP248.13-248.65 Liru zhang Huanju li Yongle qiu Yuanhang liu Xin liu Wenjing wang Screening and cellular validation of prognostic genes regulated by super enhancers in oral squamous cell carcinoma
description	Oral squamous cell carcinoma (OSCC) is the leading cause of death in patients with head and neck cancer. Reliable biomarkers to guide treatment decisions for OSCC remain scarce. The purpose of this study was to identify novel prognostic markers regulated by super enhancers in OSCC. Eight modules were obtained by weighted gene co-expression network analysis (WGCNA), among which MEblue module had the highest correlation with tumor stage, alcohol consumption and smoking. There were 41 genes regulated by super enhancers in MEblue module. Functional analysis showed that 41 super enhancer-regulated genes were involved in cancer progression. A total of twenty transcription factors of the 41 genes were predicted. Prognostic analysis of the 41 genes and the top 5 transcription factors showed that patients with high expression of AHCY, KCMF1, MANBAL and TFDP1 had a poor prognosis. Immunohistochemical analysis showed that AHCY, KCMF1 and MANBAL were highly expressed in OSCC tissue. Cellular experiment demonstrated that TFDP1 promoted AHCY, KCMF1 and MANBAL expression by binding to the super enhancers of these genes. Knockdown of TFDP1, AHCY, KCMF1 and MANBAL inhibited the proliferation of OSCC cells. In conclusion, AHCY, KCMF1 and MANBAL were recognized as super enhancer-regulated prognostic biomarkers regulated by TFDP1 in OSCC.
format	article
author	Liru zhang Huanju li Yongle qiu Yuanhang liu Xin liu Wenjing wang
author_facet	Liru zhang Huanju li Yongle qiu Yuanhang liu Xin liu Wenjing wang
author_sort	Liru zhang
title	Screening and cellular validation of prognostic genes regulated by super enhancers in oral squamous cell carcinoma
title_short	Screening and cellular validation of prognostic genes regulated by super enhancers in oral squamous cell carcinoma
title_full	Screening and cellular validation of prognostic genes regulated by super enhancers in oral squamous cell carcinoma
title_fullStr	Screening and cellular validation of prognostic genes regulated by super enhancers in oral squamous cell carcinoma
title_full_unstemmed	Screening and cellular validation of prognostic genes regulated by super enhancers in oral squamous cell carcinoma
title_sort	screening and cellular validation of prognostic genes regulated by super enhancers in oral squamous cell carcinoma
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/42ce5f144bfb494da194330b1863e2fc
work_keys_str_mv	AT liruzhang screeningandcellularvalidationofprognosticgenesregulatedbysuperenhancersinoralsquamouscellcarcinoma AT huanjuli screeningandcellularvalidationofprognosticgenesregulatedbysuperenhancersinoralsquamouscellcarcinoma AT yongleqiu screeningandcellularvalidationofprognosticgenesregulatedbysuperenhancersinoralsquamouscellcarcinoma AT yuanhangliu screeningandcellularvalidationofprognosticgenesregulatedbysuperenhancersinoralsquamouscellcarcinoma AT xinliu screeningandcellularvalidationofprognosticgenesregulatedbysuperenhancersinoralsquamouscellcarcinoma AT wenjingwang screeningandcellularvalidationofprognosticgenesregulatedbysuperenhancersinoralsquamouscellcarcinoma
_version_	1718444711231881216

Screening and cellular validation of prognostic genes regulated by super enhancers in oral squamous cell carcinoma

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