Characterisation of Plasmodium falciparum populations selected on the human endothelial receptors P-selectin, E-selectin, CD9 and CD151

Abstract The ability of the parasite Plasmodium falciparum to evade the immune system and be sequestered within human small blood vessels is responsible for severe forms of malaria. The sequestration depends on the interaction between human endothelial receptors and P. falciparum erythrocyte membran...

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Autores principales:	Nahla Galal Metwally, Ann-Kathrin Tilly, Pedro Lubiana, Lisa K. Roth, Michael Dörpinghaus, Stephan Lorenzen, Kathrin Schuldt, Susanne Witt, Anna Bachmann, Henning Tidow, Thomas Gutsmann, Thorsten Burmester, Thomas Roeder, Egbert Tannich, Iris Bruchhaus
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/42d13ef73daf4ff6bfba0bf2fa7c2781
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spelling	oai:doaj.org-article:42d13ef73daf4ff6bfba0bf2fa7c27812021-12-02T16:07:48ZCharacterisation of Plasmodium falciparum populations selected on the human endothelial receptors P-selectin, E-selectin, CD9 and CD15110.1038/s41598-017-04241-32045-2322https://doaj.org/article/42d13ef73daf4ff6bfba0bf2fa7c27812017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04241-3https://doaj.org/toc/2045-2322Abstract The ability of the parasite Plasmodium falciparum to evade the immune system and be sequestered within human small blood vessels is responsible for severe forms of malaria. The sequestration depends on the interaction between human endothelial receptors and P. falciparum erythrocyte membrane protein 1 (PfEMP1) exposed on the surface of the infected erythrocytes (IEs). In this study, the transcriptomes of parasite populations enriched for parasites that bind to human P-selectin, E-selectin, CD9 and CD151 receptors were analysed. IT4_var02 and IT4_var07 were specifically expressed in IT4 parasite populations enriched for P-selectin-binding parasites; eight var genes (IT4_var02/07/09/13/17/41/44/64) were specifically expressed in isolate populations enriched for CD9-binding parasites. Interestingly, IT4 parasite populations enriched for E-selectin- and CD151-binding parasites showed identical expression profiles to those of a parasite population exposed to wild-type CHO-745 cells. The same phenomenon was observed for the 3D7 isolate population enriched for binding to P-selectin, E-selectin, CD9 and CD151. This implies that the corresponding ligands for these receptors have either weak binding capacity or do not exist on the IE surface. Conclusively, this work expanded our understanding of P. falciparum adhesive interactions, through the identification of var transcripts that are enriched within the selected parasite populations.Nahla Galal MetwallyAnn-Kathrin TillyPedro LubianaLisa K. RothMichael DörpinghausStephan LorenzenKathrin SchuldtSusanne WittAnna BachmannHenning TidowThomas GutsmannThorsten BurmesterThomas RoederEgbert TannichIris BruchhausNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nahla Galal Metwally Ann-Kathrin Tilly Pedro Lubiana Lisa K. Roth Michael Dörpinghaus Stephan Lorenzen Kathrin Schuldt Susanne Witt Anna Bachmann Henning Tidow Thomas Gutsmann Thorsten Burmester Thomas Roeder Egbert Tannich Iris Bruchhaus Characterisation of Plasmodium falciparum populations selected on the human endothelial receptors P-selectin, E-selectin, CD9 and CD151
description	Abstract The ability of the parasite Plasmodium falciparum to evade the immune system and be sequestered within human small blood vessels is responsible for severe forms of malaria. The sequestration depends on the interaction between human endothelial receptors and P. falciparum erythrocyte membrane protein 1 (PfEMP1) exposed on the surface of the infected erythrocytes (IEs). In this study, the transcriptomes of parasite populations enriched for parasites that bind to human P-selectin, E-selectin, CD9 and CD151 receptors were analysed. IT4_var02 and IT4_var07 were specifically expressed in IT4 parasite populations enriched for P-selectin-binding parasites; eight var genes (IT4_var02/07/09/13/17/41/44/64) were specifically expressed in isolate populations enriched for CD9-binding parasites. Interestingly, IT4 parasite populations enriched for E-selectin- and CD151-binding parasites showed identical expression profiles to those of a parasite population exposed to wild-type CHO-745 cells. The same phenomenon was observed for the 3D7 isolate population enriched for binding to P-selectin, E-selectin, CD9 and CD151. This implies that the corresponding ligands for these receptors have either weak binding capacity or do not exist on the IE surface. Conclusively, this work expanded our understanding of P. falciparum adhesive interactions, through the identification of var transcripts that are enriched within the selected parasite populations.
format	article
author	Nahla Galal Metwally Ann-Kathrin Tilly Pedro Lubiana Lisa K. Roth Michael Dörpinghaus Stephan Lorenzen Kathrin Schuldt Susanne Witt Anna Bachmann Henning Tidow Thomas Gutsmann Thorsten Burmester Thomas Roeder Egbert Tannich Iris Bruchhaus
author_facet	Nahla Galal Metwally Ann-Kathrin Tilly Pedro Lubiana Lisa K. Roth Michael Dörpinghaus Stephan Lorenzen Kathrin Schuldt Susanne Witt Anna Bachmann Henning Tidow Thomas Gutsmann Thorsten Burmester Thomas Roeder Egbert Tannich Iris Bruchhaus
author_sort	Nahla Galal Metwally
title	Characterisation of Plasmodium falciparum populations selected on the human endothelial receptors P-selectin, E-selectin, CD9 and CD151
title_short	Characterisation of Plasmodium falciparum populations selected on the human endothelial receptors P-selectin, E-selectin, CD9 and CD151
title_full	Characterisation of Plasmodium falciparum populations selected on the human endothelial receptors P-selectin, E-selectin, CD9 and CD151
title_fullStr	Characterisation of Plasmodium falciparum populations selected on the human endothelial receptors P-selectin, E-selectin, CD9 and CD151
title_full_unstemmed	Characterisation of Plasmodium falciparum populations selected on the human endothelial receptors P-selectin, E-selectin, CD9 and CD151
title_sort	characterisation of plasmodium falciparum populations selected on the human endothelial receptors p-selectin, e-selectin, cd9 and cd151
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/42d13ef73daf4ff6bfba0bf2fa7c2781
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Characterisation of Plasmodium falciparum populations selected on the human endothelial receptors P-selectin, E-selectin, CD9 and CD151

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