MicroRNA-148 as a negative regulator of the common TLR adaptor mediates inflammatory response in teleost fish

Abstract MicroRNAs are small endogenous noncoding RNAs implicating in the regulation of diverse biological processes, including proliferation, differentiation, cancer, apoptosis, and viral infections. MicroRNAs regulate gene expression by either mRNA degradation or inhibition of protein translation....

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Autores principales:	Qing Chu, Yunhang Gao, Dekun Bi, Tianjun Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/42d3f5a684594b8eafa7f6b73c1b49c1
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spelling	oai:doaj.org-article:42d3f5a684594b8eafa7f6b73c1b49c12021-12-02T12:30:53ZMicroRNA-148 as a negative regulator of the common TLR adaptor mediates inflammatory response in teleost fish10.1038/s41598-017-04354-92045-2322https://doaj.org/article/42d3f5a684594b8eafa7f6b73c1b49c12017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04354-9https://doaj.org/toc/2045-2322Abstract MicroRNAs are small endogenous noncoding RNAs implicating in the regulation of diverse biological processes, including proliferation, differentiation, cancer, apoptosis, and viral infections. MicroRNAs regulate gene expression by either mRNA degradation or inhibition of protein translation. Although microRNAs have emerged as important controller involved in regulation of inflammatory response, the microRNA-mediated regulatory mechanism remains less clear in teleost. Here, we report that miR-148 targets MyD88 and down-regulates its expression by inhibition protein translation rather than degradation mRNA in miiuy croaker. Additionally, we found that miR-148 was significantly upregulated in miiuy croaker after treated with Vibro harveyi, as well as LPS. Overexpression of miR-148 inhibited LPS-induced inflammatory cytokines production, such as IL-6 and IL-1β, which then avoid excessive inflammation response. miR-148 has also been identified to suppress NF-κB pathway through targeting and repressing MyD88 expression. Taken together, our findings indicate that miR-148 participates in bacteria-induced inflammatory response and act as a negative regulator for MyD88-mediated NF-κB signaling, which may clarify the mechanism of microRNAs for avoiding excessive inflammation in teleost fish.Qing ChuYunhang GaoDekun BiTianjun XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qing Chu Yunhang Gao Dekun Bi Tianjun Xu MicroRNA-148 as a negative regulator of the common TLR adaptor mediates inflammatory response in teleost fish
description	Abstract MicroRNAs are small endogenous noncoding RNAs implicating in the regulation of diverse biological processes, including proliferation, differentiation, cancer, apoptosis, and viral infections. MicroRNAs regulate gene expression by either mRNA degradation or inhibition of protein translation. Although microRNAs have emerged as important controller involved in regulation of inflammatory response, the microRNA-mediated regulatory mechanism remains less clear in teleost. Here, we report that miR-148 targets MyD88 and down-regulates its expression by inhibition protein translation rather than degradation mRNA in miiuy croaker. Additionally, we found that miR-148 was significantly upregulated in miiuy croaker after treated with Vibro harveyi, as well as LPS. Overexpression of miR-148 inhibited LPS-induced inflammatory cytokines production, such as IL-6 and IL-1β, which then avoid excessive inflammation response. miR-148 has also been identified to suppress NF-κB pathway through targeting and repressing MyD88 expression. Taken together, our findings indicate that miR-148 participates in bacteria-induced inflammatory response and act as a negative regulator for MyD88-mediated NF-κB signaling, which may clarify the mechanism of microRNAs for avoiding excessive inflammation in teleost fish.
format	article
author	Qing Chu Yunhang Gao Dekun Bi Tianjun Xu
author_facet	Qing Chu Yunhang Gao Dekun Bi Tianjun Xu
author_sort	Qing Chu
title	MicroRNA-148 as a negative regulator of the common TLR adaptor mediates inflammatory response in teleost fish
title_short	MicroRNA-148 as a negative regulator of the common TLR adaptor mediates inflammatory response in teleost fish
title_full	MicroRNA-148 as a negative regulator of the common TLR adaptor mediates inflammatory response in teleost fish
title_fullStr	MicroRNA-148 as a negative regulator of the common TLR adaptor mediates inflammatory response in teleost fish
title_full_unstemmed	MicroRNA-148 as a negative regulator of the common TLR adaptor mediates inflammatory response in teleost fish
title_sort	microrna-148 as a negative regulator of the common tlr adaptor mediates inflammatory response in teleost fish
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/42d3f5a684594b8eafa7f6b73c1b49c1
work_keys_str_mv	AT qingchu microrna148asanegativeregulatorofthecommontlradaptormediatesinflammatoryresponseinteleostfish AT yunhanggao microrna148asanegativeregulatorofthecommontlradaptormediatesinflammatoryresponseinteleostfish AT dekunbi microrna148asanegativeregulatorofthecommontlradaptormediatesinflammatoryresponseinteleostfish AT tianjunxu microrna148asanegativeregulatorofthecommontlradaptormediatesinflammatoryresponseinteleostfish
_version_	1718394273927266304

MicroRNA-148 as a negative regulator of the common TLR adaptor mediates inflammatory response in teleost fish

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