Membrane Perfusion of Hydrophobic Substances Around Channels Embedded in the Contact Bubble Bilayer

Abstract In fluidic biomembranes, lipids and membrane proteins diffuse restlessly, and lipid compositions change steadily. To mimic dynamic behavior of the biomembranes, a method for introducing rapid changes in the constituents in the lipid bilayer was developed. In contact bubble bilayers (CBB), a...

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Autores principales:	Masayuki Iwamoto, Shigetoshi Oiki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/42d85d78e90b47e7820bbb1db520c530
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spelling	oai:doaj.org-article:42d85d78e90b47e7820bbb1db520c5302021-12-02T16:06:08ZMembrane Perfusion of Hydrophobic Substances Around Channels Embedded in the Contact Bubble Bilayer10.1038/s41598-017-07048-42045-2322https://doaj.org/article/42d85d78e90b47e7820bbb1db520c5302017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07048-4https://doaj.org/toc/2045-2322Abstract In fluidic biomembranes, lipids and membrane proteins diffuse restlessly, and lipid compositions change steadily. To mimic dynamic behavior of the biomembranes, a method for introducing rapid changes in the constituents in the lipid bilayer was developed. In contact bubble bilayers (CBB), as a water-in-oil droplet bilayer system, the bilayer hydrophobic interior is contiguous with the bulk oil phase. Making use of this geometrical feature as an access route, hydrophobic substances were administered into the bilayer. Polytheonamide B, a cytotoxic hydrophobic peptide, was applied, and oriented incorporation and relevant single-channel current recordings were enabled. Nystatin was pre-loaded in the CBB, and sterol perfusion exhibited slow development of the macroscopic current. On the contrary, the reconstituted KcsA potassium channels immediately attenuate the channel activity when cholesterol was applied. This oil-phase route in the CBB allows rapid perfusion of hydrophobic substances around the bilayer-embedded channels during continuous recordings of channel currents.Masayuki IwamotoShigetoshi OikiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Masayuki Iwamoto Shigetoshi Oiki Membrane Perfusion of Hydrophobic Substances Around Channels Embedded in the Contact Bubble Bilayer
description	Abstract In fluidic biomembranes, lipids and membrane proteins diffuse restlessly, and lipid compositions change steadily. To mimic dynamic behavior of the biomembranes, a method for introducing rapid changes in the constituents in the lipid bilayer was developed. In contact bubble bilayers (CBB), as a water-in-oil droplet bilayer system, the bilayer hydrophobic interior is contiguous with the bulk oil phase. Making use of this geometrical feature as an access route, hydrophobic substances were administered into the bilayer. Polytheonamide B, a cytotoxic hydrophobic peptide, was applied, and oriented incorporation and relevant single-channel current recordings were enabled. Nystatin was pre-loaded in the CBB, and sterol perfusion exhibited slow development of the macroscopic current. On the contrary, the reconstituted KcsA potassium channels immediately attenuate the channel activity when cholesterol was applied. This oil-phase route in the CBB allows rapid perfusion of hydrophobic substances around the bilayer-embedded channels during continuous recordings of channel currents.
format	article
author	Masayuki Iwamoto Shigetoshi Oiki
author_facet	Masayuki Iwamoto Shigetoshi Oiki
author_sort	Masayuki Iwamoto
title	Membrane Perfusion of Hydrophobic Substances Around Channels Embedded in the Contact Bubble Bilayer
title_short	Membrane Perfusion of Hydrophobic Substances Around Channels Embedded in the Contact Bubble Bilayer
title_full	Membrane Perfusion of Hydrophobic Substances Around Channels Embedded in the Contact Bubble Bilayer
title_fullStr	Membrane Perfusion of Hydrophobic Substances Around Channels Embedded in the Contact Bubble Bilayer
title_full_unstemmed	Membrane Perfusion of Hydrophobic Substances Around Channels Embedded in the Contact Bubble Bilayer
title_sort	membrane perfusion of hydrophobic substances around channels embedded in the contact bubble bilayer
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/42d85d78e90b47e7820bbb1db520c530
work_keys_str_mv	AT masayukiiwamoto membraneperfusionofhydrophobicsubstancesaroundchannelsembeddedinthecontactbubblebilayer AT shigetoshioiki membraneperfusionofhydrophobicsubstancesaroundchannelsembeddedinthecontactbubblebilayer
_version_	1718385082595540992

Membrane Perfusion of Hydrophobic Substances Around Channels Embedded in the Contact Bubble Bilayer

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