The Regeneration of Large-Sized and Vascularized Adipose Tissue Using a Tailored Elastic Scaffold and dECM Hydrogels

A dome-shaped elastic poly(<span style="font-variant: small-caps;">l</span>-lactide-<i>co</i>-caprolactone) (PLCL) scaffold with a channel and pore structure was fabricated by a combinative method of 3D printing technology and the gel pressing method (13 mm in diame...

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Autores principales:	Su Hee Kim, Donghak Kim, Misun Cha, Soo Hyun Kim, Youngmee Jung
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	adipose tissue regeneration decellularized extracellular matrix (dECM) hydrogel patient-specific elastic scaffolds angiogenesis tissue engineering Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/42db2ab756a54feca5e001ccdf140d38
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spelling	oai:doaj.org-article:42db2ab756a54feca5e001ccdf140d382021-11-25T17:57:50ZThe Regeneration of Large-Sized and Vascularized Adipose Tissue Using a Tailored Elastic Scaffold and dECM Hydrogels10.3390/ijms2222125601422-00671661-6596https://doaj.org/article/42db2ab756a54feca5e001ccdf140d382021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12560https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067A dome-shaped elastic poly(<span style="font-variant: small-caps;">l</span>-lactide-<i>co</i>-caprolactone) (PLCL) scaffold with a channel and pore structure was fabricated by a combinative method of 3D printing technology and the gel pressing method (13 mm in diameter and 6.5 mm in thickness) for patient-specific regeneration. The PLCL scaffold was combined with adipose decellularized extracellular matrix (adECM) and heart decellularized extracellular matrix (hdECM) hydrogels and human adipose-derived stem cells (hADSCs) to promote adipogenesis and angiogenesis. These scaffolds had mechanical properties similar to those of native adipose tissue for improved tissue regeneration. The results of the in vitro real-time PCR showed that the dECM hydrogel mixture induces adipogenesis. In addition, the in vivo study at 12 weeks demonstrated that the tissue-engineered PLCL scaffolds containing the hydrogel mixture (hdECM/adECM (80:20)) and hADSCs promoted angiogenesis and adipose tissue formation, and suppressed apoptosis. Therefore, we expect that our constructs will be clinically applicable as material for the regeneration of patient-specific large-sized adipose tissue.Su Hee KimDonghak KimMisun ChaSoo Hyun KimYoungmee JungMDPI AGarticleadipose tissue regenerationdecellularized extracellular matrix (dECM) hydrogelpatient-specific elastic scaffoldsangiogenesistissue engineeringBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12560, p 12560 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adipose tissue regeneration decellularized extracellular matrix (dECM) hydrogel patient-specific elastic scaffolds angiogenesis tissue engineering Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	adipose tissue regeneration decellularized extracellular matrix (dECM) hydrogel patient-specific elastic scaffolds angiogenesis tissue engineering Biology (General) QH301-705.5 Chemistry QD1-999 Su Hee Kim Donghak Kim Misun Cha Soo Hyun Kim Youngmee Jung The Regeneration of Large-Sized and Vascularized Adipose Tissue Using a Tailored Elastic Scaffold and dECM Hydrogels
description	A dome-shaped elastic poly(<span style="font-variant: small-caps;">l</span>-lactide-<i>co</i>-caprolactone) (PLCL) scaffold with a channel and pore structure was fabricated by a combinative method of 3D printing technology and the gel pressing method (13 mm in diameter and 6.5 mm in thickness) for patient-specific regeneration. The PLCL scaffold was combined with adipose decellularized extracellular matrix (adECM) and heart decellularized extracellular matrix (hdECM) hydrogels and human adipose-derived stem cells (hADSCs) to promote adipogenesis and angiogenesis. These scaffolds had mechanical properties similar to those of native adipose tissue for improved tissue regeneration. The results of the in vitro real-time PCR showed that the dECM hydrogel mixture induces adipogenesis. In addition, the in vivo study at 12 weeks demonstrated that the tissue-engineered PLCL scaffolds containing the hydrogel mixture (hdECM/adECM (80:20)) and hADSCs promoted angiogenesis and adipose tissue formation, and suppressed apoptosis. Therefore, we expect that our constructs will be clinically applicable as material for the regeneration of patient-specific large-sized adipose tissue.
format	article
author	Su Hee Kim Donghak Kim Misun Cha Soo Hyun Kim Youngmee Jung
author_facet	Su Hee Kim Donghak Kim Misun Cha Soo Hyun Kim Youngmee Jung
author_sort	Su Hee Kim
title	The Regeneration of Large-Sized and Vascularized Adipose Tissue Using a Tailored Elastic Scaffold and dECM Hydrogels
title_short	The Regeneration of Large-Sized and Vascularized Adipose Tissue Using a Tailored Elastic Scaffold and dECM Hydrogels
title_full	The Regeneration of Large-Sized and Vascularized Adipose Tissue Using a Tailored Elastic Scaffold and dECM Hydrogels
title_fullStr	The Regeneration of Large-Sized and Vascularized Adipose Tissue Using a Tailored Elastic Scaffold and dECM Hydrogels
title_full_unstemmed	The Regeneration of Large-Sized and Vascularized Adipose Tissue Using a Tailored Elastic Scaffold and dECM Hydrogels
title_sort	regeneration of large-sized and vascularized adipose tissue using a tailored elastic scaffold and decm hydrogels
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/42db2ab756a54feca5e001ccdf140d38
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The Regeneration of Large-Sized and Vascularized Adipose Tissue Using a Tailored Elastic Scaffold and dECM Hydrogels

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