Drought Tolerant <i>Enterobacter</i> sp./<i>Leclercia adecarboxylata</i> Secretes Indole-3-acetic Acid and Other Biomolecules and Enhances the Biological Attributes of <i>Vigna radiata</i> (L.) R. Wilczek in Water Deficit Conditions

Drought Tolerant <i>Enterobacter</i> sp./<i>Leclercia adecarboxylata</i> Secretes Indole-3-acetic Acid and Other Biomolecules and Enhances the Biological Attributes of <i>Vigna radiata</i> (L.) R. Wilczek in Water Deficit Conditions

Drought or water stress is a limiting factor that hampers the growth and yield of edible crops. Drought-tolerant plant growth-promoting rhizobacteria (PGPR) can mitigate water stress in crops by synthesizing multiple bioactive molecules. Here, strain PAB19 recovered from rhizospheric soil was bioche...

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Autores principales: Bilal Ahmed, Mohammad Shahid, Asad Syed, Vishnu D. Rajput, Abdallah M. Elgorban, Tatiana Minkina, Ali H. Bahkali, Jintae Lee
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
drought stress
<i>Enterobacter</i> sp./<i>L. adecarboxylata</i>
growth regulating substances
<i>Vigna radiata</i> (L.) R. Wilczek
growth improvement
gas exchange parameters
Biology (General)
QH301-705.5
Acceso en línea:https://doaj.org/article/42dbefafbc0b476db5f867b761334050
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spelling oai:doaj.org-article:42dbefafbc0b476db5f867b7613340502021-11-25T16:47:28ZDrought Tolerant <i>Enterobacter</i> sp./<i>Leclercia adecarboxylata</i> Secretes Indole-3-acetic Acid and Other Biomolecules and Enhances the Biological Attributes of <i>Vigna radiata</i> (L.) R. Wilczek in Water Deficit Conditions10.3390/biology101111492079-7737https://doaj.org/article/42dbefafbc0b476db5f867b7613340502021-11-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1149https://doaj.org/toc/2079-7737Drought or water stress is a limiting factor that hampers the growth and yield of edible crops. Drought-tolerant plant growth-promoting rhizobacteria (PGPR) can mitigate water stress in crops by synthesizing multiple bioactive molecules. Here, strain PAB19 recovered from rhizospheric soil was biochemically and molecularly characterized, and identified as <i>Enterobacter</i> sp./<i>Leclercia adecarboxylata</i> (MT672579.1). Strain PAB19 tolerated an exceptionally high level of drought (18% PEG-6000) and produced indole-3-acetic acid (176.2 ± 5.6 µg mL<sup>−1</sup>), ACC deaminase (56.6 ± 5.0 µg mL<sup>−1</sup>), salicylic acid (42.5 ± 3.0 µg mL<sup>−1</sup>), 2,3-dihydroxy benzoic acid (DHBA) (44.3 ± 2.3 µg mL<sup>−1</sup>), exopolysaccharide (204 ± 14.7 µg mL<sup>−1</sup>), alginate (82.3 ± 6.5 µg mL<sup>−1</sup>), and solubilized tricalcium phosphate (98.3 ± 3.5 µg mL<sup>−1</sup>), in the presence of 15% polyethylene glycol. Furthermore, strain PAB19 alleviated water stress and significantly (<i>p</i> ≤ 0.05) improved the overall growth and biochemical attributes of <i>Vigna radiata</i> (L.) R. Wilczek. For instance, at 2% PEG stress, PAB19 inoculation maximally increased germination, root dry biomass, leaf carotenoid content, nodule biomass, leghaemoglobin (LHb) content, leaf water potential (ΨL), membrane stability index (MSI), and pod yield by 10%, 7%, 14%, 38%, 9%, 17%, 11%, and 11%, respectively, over un-inoculated plants. Additionally, PAB19 inoculation reduced two stressor metabolites, proline and malondialdehyde, and antioxidant enzymes (POD, SOD, CAT, and GR) levels in <i>V. radiata</i> foliage in water stress conditions. Following inoculation of strain PAB19 with 15% PEG in soil, stomatal conductance, intercellular CO<sub>2</sub> concentration, transpiration rate, water vapor deficit, intrinsic water use efficiency, and photosynthetic rate were significantly improved by 12%, 8%, 42%, 10%, 9% and 16%, respectively. Rhizospheric CFU counts of PAB19 were 2.33 and 2.11 log CFU g<sup>−1</sup> after treatment with 15% PEG solution and 8.46 and 6.67 log CFU g<sup>−1</sup> for untreated controls at 40 and 80 DAS, respectively. Conclusively, this study suggests the potential of <i>Enterobacter</i> sp./<i>L. adecarboxylata</i> PAB19 to alleviate water stress by improving the biological and biochemical features and of <i>V. radiata</i> under water-deficit conditions.Bilal AhmedMohammad ShahidAsad SyedVishnu D. RajputAbdallah M. ElgorbanTatiana MinkinaAli H. BahkaliJintae LeeMDPI AGarticledrought stress<i>Enterobacter</i> sp./<i>L. adecarboxylata</i>growth regulating substances<i>Vigna radiata</i> (L.) R. Wilczekgrowth improvementgas exchange parametersBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1149, p 1149 (2021)
institution DOAJ
collection DOAJ
language EN
topic drought stress
<i>Enterobacter</i> sp./<i>L. adecarboxylata</i>
growth regulating substances
<i>Vigna radiata</i> (L.) R. Wilczek
growth improvement
gas exchange parameters
Biology (General)
QH301-705.5
spellingShingle drought stress
<i>Enterobacter</i> sp./<i>L. adecarboxylata</i>
growth regulating substances
<i>Vigna radiata</i> (L.) R. Wilczek
growth improvement
gas exchange parameters
Biology (General)
QH301-705.5
Bilal Ahmed
Mohammad Shahid
Asad Syed
Vishnu D. Rajput
Abdallah M. Elgorban
Tatiana Minkina
Ali H. Bahkali
Jintae Lee
Drought Tolerant <i>Enterobacter</i> sp./<i>Leclercia adecarboxylata</i> Secretes Indole-3-acetic Acid and Other Biomolecules and Enhances the Biological Attributes of <i>Vigna radiata</i> (L.) R. Wilczek in Water Deficit Conditions
description Drought or water stress is a limiting factor that hampers the growth and yield of edible crops. Drought-tolerant plant growth-promoting rhizobacteria (PGPR) can mitigate water stress in crops by synthesizing multiple bioactive molecules. Here, strain PAB19 recovered from rhizospheric soil was biochemically and molecularly characterized, and identified as <i>Enterobacter</i> sp./<i>Leclercia adecarboxylata</i> (MT672579.1). Strain PAB19 tolerated an exceptionally high level of drought (18% PEG-6000) and produced indole-3-acetic acid (176.2 ± 5.6 µg mL<sup>−1</sup>), ACC deaminase (56.6 ± 5.0 µg mL<sup>−1</sup>), salicylic acid (42.5 ± 3.0 µg mL<sup>−1</sup>), 2,3-dihydroxy benzoic acid (DHBA) (44.3 ± 2.3 µg mL<sup>−1</sup>), exopolysaccharide (204 ± 14.7 µg mL<sup>−1</sup>), alginate (82.3 ± 6.5 µg mL<sup>−1</sup>), and solubilized tricalcium phosphate (98.3 ± 3.5 µg mL<sup>−1</sup>), in the presence of 15% polyethylene glycol. Furthermore, strain PAB19 alleviated water stress and significantly (<i>p</i> ≤ 0.05) improved the overall growth and biochemical attributes of <i>Vigna radiata</i> (L.) R. Wilczek. For instance, at 2% PEG stress, PAB19 inoculation maximally increased germination, root dry biomass, leaf carotenoid content, nodule biomass, leghaemoglobin (LHb) content, leaf water potential (ΨL), membrane stability index (MSI), and pod yield by 10%, 7%, 14%, 38%, 9%, 17%, 11%, and 11%, respectively, over un-inoculated plants. Additionally, PAB19 inoculation reduced two stressor metabolites, proline and malondialdehyde, and antioxidant enzymes (POD, SOD, CAT, and GR) levels in <i>V. radiata</i> foliage in water stress conditions. Following inoculation of strain PAB19 with 15% PEG in soil, stomatal conductance, intercellular CO<sub>2</sub> concentration, transpiration rate, water vapor deficit, intrinsic water use efficiency, and photosynthetic rate were significantly improved by 12%, 8%, 42%, 10%, 9% and 16%, respectively. Rhizospheric CFU counts of PAB19 were 2.33 and 2.11 log CFU g<sup>−1</sup> after treatment with 15% PEG solution and 8.46 and 6.67 log CFU g<sup>−1</sup> for untreated controls at 40 and 80 DAS, respectively. Conclusively, this study suggests the potential of <i>Enterobacter</i> sp./<i>L. adecarboxylata</i> PAB19 to alleviate water stress by improving the biological and biochemical features and of <i>V. radiata</i> under water-deficit conditions.
format article
author Bilal Ahmed
Mohammad Shahid
Asad Syed
Vishnu D. Rajput
Abdallah M. Elgorban
Tatiana Minkina
Ali H. Bahkali
Jintae Lee
author_facet Bilal Ahmed
Mohammad Shahid
Asad Syed
Vishnu D. Rajput
Abdallah M. Elgorban
Tatiana Minkina
Ali H. Bahkali
Jintae Lee
author_sort Bilal Ahmed
title Drought Tolerant <i>Enterobacter</i> sp./<i>Leclercia adecarboxylata</i> Secretes Indole-3-acetic Acid and Other Biomolecules and Enhances the Biological Attributes of <i>Vigna radiata</i> (L.) R. Wilczek in Water Deficit Conditions
title_short Drought Tolerant <i>Enterobacter</i> sp./<i>Leclercia adecarboxylata</i> Secretes Indole-3-acetic Acid and Other Biomolecules and Enhances the Biological Attributes of <i>Vigna radiata</i> (L.) R. Wilczek in Water Deficit Conditions
title_full Drought Tolerant <i>Enterobacter</i> sp./<i>Leclercia adecarboxylata</i> Secretes Indole-3-acetic Acid and Other Biomolecules and Enhances the Biological Attributes of <i>Vigna radiata</i> (L.) R. Wilczek in Water Deficit Conditions
title_fullStr Drought Tolerant <i>Enterobacter</i> sp./<i>Leclercia adecarboxylata</i> Secretes Indole-3-acetic Acid and Other Biomolecules and Enhances the Biological Attributes of <i>Vigna radiata</i> (L.) R. Wilczek in Water Deficit Conditions
title_full_unstemmed Drought Tolerant <i>Enterobacter</i> sp./<i>Leclercia adecarboxylata</i> Secretes Indole-3-acetic Acid and Other Biomolecules and Enhances the Biological Attributes of <i>Vigna radiata</i> (L.) R. Wilczek in Water Deficit Conditions
title_sort drought tolerant <i>enterobacter</i> sp./<i>leclercia adecarboxylata</i> secretes indole-3-acetic acid and other biomolecules and enhances the biological attributes of <i>vigna radiata</i> (l.) r. wilczek in water deficit conditions
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/42dbefafbc0b476db5f867b761334050
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