Resistance Training Modulates the Matrix Metalloproteinase-2 Activity in Different Trabecular Bones in Aged Rats

Ivo Vieira de Sousa Neto,1 João Luiz Quaglioti Durigan,1,2 Gonçalo Carreiro de Farias Junior,2 Fabio Henrique Bogni,3 Amanda Lima Ruivo,1 Juliana Oliveira de Araújo,1 Keico Okino Nonaka,3 Heloísa Selistre-de-Araújo,3 Rita de Cássia Ma...

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Autores principales:	de Sousa Neto IV, Durigan JLQ, Carreiro de Farias Junior G, Bogni FH, Ruivo AL, Araújo JO, Nonaka KO, Selistre-de-Araújo H, Marqueti RC
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	senescent skeletal system remodeling matrix metallopeptidase exercise Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/42dd847d07874929a899fb124e9be1fb
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spelling	oai:doaj.org-article:42dd847d07874929a899fb124e9be1fb2021-12-02T15:13:18ZResistance Training Modulates the Matrix Metalloproteinase-2 Activity in Different Trabecular Bones in Aged Rats1178-1998https://doaj.org/article/42dd847d07874929a899fb124e9be1fb2021-01-01T00:00:00Zhttps://www.dovepress.com/resistance-training-modulates-the-matrix-metalloproteinase-2-activity--peer-reviewed-article-CIAhttps://doaj.org/toc/1178-1998Ivo Vieira de Sousa Neto,1 João Luiz Quaglioti Durigan,1,2 Gonçalo Carreiro de Farias Junior,2 Fabio Henrique Bogni,3 Amanda Lima Ruivo,1 Juliana Oliveira de Araújo,1 Keico Okino Nonaka,3 Heloísa Selistre-de-Araújo,3 Rita de Cássia Marqueti1,2 1Laboratory of Molecular Analysis, Graduate Program of Sciences and Technology of Health, Universidade de Brasília, Distrito Federal, Brazil; 2Graduate Program in Rehabilitation Sciences, Universidade de Brasília, Distrito Federal, Brazil; 3Department of Physiological Sciences, Universidade Federal de São Carlos, São Paulo, BrazilCorrespondence: Ivo Vieira de Sousa Neto; Rita de Cássia MarquetiLaboratory of Molecular Analysis, Graduate Program of Sciences and Technology of Health, Universidade de Brasília, Distrito Federal, BrazilEmail ivoneto04@hotmail.com; marqueti@gmail.comBackground: Aging decreases osteogenic ability, inducing harmful effects on the bone extracellular matrix (ECM), while exercise training has been indicated as a tool to counteract bone disorders related to advancing age. The modulation of bone ECM is regulated by several types of matrix metalloproteinase (MMP); however, MMP-2 activity in different trabecular bones in response to resistance training (RT) has been neglected. Remodeling differs in different bones under the application of the same mechanical loading. Thus, we investigated the effects of 12 weeks of RT on MMP-2 activity in the lumbar vertebra (L6), tibia, and femur of young (3 months) and older rats (21 months).Methods: Twenty Wistar rats were divided into four groups (five animals per group): young sedentary or trained and older sedentary or trained. The 12-week RT consisted of climbing a 1.1-m vertical ladder three times per week with progressive weights secured to the animals’ tails. The animals were killed 48 h after the end of the experimental period. The MMP-2 activity was assessed by the zymography method.Results: The aging process induced lower MMP-2 activity in the lumbar vertebrae and tibia (p=0.01). RT upregulated pro, intermediate, and active MMP-2 activity in the tibia of young rats (p=0.001). RT also upregulated pro and active MMP-2 activity in the lumbar vertebrae and tibia with advancing age (p=0.01). There was no significant difference (p> 0.05) between groups for MMP-2 of the femur, regardless of age and RT.Conclusion: The aging process impairs MMP-2 activity, but RT is a potential therapeutic approach to minimize the deleterious effects of ECM degeneration in different aged bones. Distinct MMP-2 responses to exercise training may result in specific remodeling processes.Keywords: senescent, skeletal system, remodeling, matrix metalloproteinase, exercisede Sousa Neto IVDurigan JLQCarreiro de Farias Junior GBogni FHRuivo ALAraújo JONonaka KOSelistre-de-Araújo HMarqueti RCDove Medical Pressarticlesenescentskeletal systemremodelingmatrix metallopeptidaseexerciseGeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 16, Pp 71-81 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	senescent skeletal system remodeling matrix metallopeptidase exercise Geriatrics RC952-954.6
spellingShingle	senescent skeletal system remodeling matrix metallopeptidase exercise Geriatrics RC952-954.6 de Sousa Neto IV Durigan JLQ Carreiro de Farias Junior G Bogni FH Ruivo AL Araújo JO Nonaka KO Selistre-de-Araújo H Marqueti RC Resistance Training Modulates the Matrix Metalloproteinase-2 Activity in Different Trabecular Bones in Aged Rats
description	Ivo Vieira de Sousa Neto,1 João Luiz Quaglioti Durigan,1,2 Gonçalo Carreiro de Farias Junior,2 Fabio Henrique Bogni,3 Amanda Lima Ruivo,1 Juliana Oliveira de Araújo,1 Keico Okino Nonaka,3 Heloísa Selistre-de-Araújo,3 Rita de Cássia Marqueti1,2 1Laboratory of Molecular Analysis, Graduate Program of Sciences and Technology of Health, Universidade de Brasília, Distrito Federal, Brazil; 2Graduate Program in Rehabilitation Sciences, Universidade de Brasília, Distrito Federal, Brazil; 3Department of Physiological Sciences, Universidade Federal de São Carlos, São Paulo, BrazilCorrespondence: Ivo Vieira de Sousa Neto; Rita de Cássia MarquetiLaboratory of Molecular Analysis, Graduate Program of Sciences and Technology of Health, Universidade de Brasília, Distrito Federal, BrazilEmail ivoneto04@hotmail.com; marqueti@gmail.comBackground: Aging decreases osteogenic ability, inducing harmful effects on the bone extracellular matrix (ECM), while exercise training has been indicated as a tool to counteract bone disorders related to advancing age. The modulation of bone ECM is regulated by several types of matrix metalloproteinase (MMP); however, MMP-2 activity in different trabecular bones in response to resistance training (RT) has been neglected. Remodeling differs in different bones under the application of the same mechanical loading. Thus, we investigated the effects of 12 weeks of RT on MMP-2 activity in the lumbar vertebra (L6), tibia, and femur of young (3 months) and older rats (21 months).Methods: Twenty Wistar rats were divided into four groups (five animals per group): young sedentary or trained and older sedentary or trained. The 12-week RT consisted of climbing a 1.1-m vertical ladder three times per week with progressive weights secured to the animals’ tails. The animals were killed 48 h after the end of the experimental period. The MMP-2 activity was assessed by the zymography method.Results: The aging process induced lower MMP-2 activity in the lumbar vertebrae and tibia (p=0.01). RT upregulated pro, intermediate, and active MMP-2 activity in the tibia of young rats (p=0.001). RT also upregulated pro and active MMP-2 activity in the lumbar vertebrae and tibia with advancing age (p=0.01). There was no significant difference (p> 0.05) between groups for MMP-2 of the femur, regardless of age and RT.Conclusion: The aging process impairs MMP-2 activity, but RT is a potential therapeutic approach to minimize the deleterious effects of ECM degeneration in different aged bones. Distinct MMP-2 responses to exercise training may result in specific remodeling processes.Keywords: senescent, skeletal system, remodeling, matrix metalloproteinase, exercise
format	article
author	de Sousa Neto IV Durigan JLQ Carreiro de Farias Junior G Bogni FH Ruivo AL Araújo JO Nonaka KO Selistre-de-Araújo H Marqueti RC
author_facet	de Sousa Neto IV Durigan JLQ Carreiro de Farias Junior G Bogni FH Ruivo AL Araújo JO Nonaka KO Selistre-de-Araújo H Marqueti RC
author_sort	de Sousa Neto IV
title	Resistance Training Modulates the Matrix Metalloproteinase-2 Activity in Different Trabecular Bones in Aged Rats
title_short	Resistance Training Modulates the Matrix Metalloproteinase-2 Activity in Different Trabecular Bones in Aged Rats
title_full	Resistance Training Modulates the Matrix Metalloproteinase-2 Activity in Different Trabecular Bones in Aged Rats
title_fullStr	Resistance Training Modulates the Matrix Metalloproteinase-2 Activity in Different Trabecular Bones in Aged Rats
title_full_unstemmed	Resistance Training Modulates the Matrix Metalloproteinase-2 Activity in Different Trabecular Bones in Aged Rats
title_sort	resistance training modulates the matrix metalloproteinase-2 activity in different trabecular bones in aged rats
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/42dd847d07874929a899fb124e9be1fb
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Resistance Training Modulates the Matrix Metalloproteinase-2 Activity in Different Trabecular Bones in Aged Rats

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