Detection of crosslinks within and between proteins by LC-MALDI-TOFTOF and the software FINDX to reduce the MSMS-data to acquire for validation.
Lysine-specific chemical crosslinking in combination with mass spectrometry is emerging as a tool for the structural characterization of protein complexes and protein-protein interactions. After tryptic digestion of crosslinked proteins there are thousands of peptides amenable to MSMS, of which only...
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Autores principales: | Christopher A G Söderberg, Wietske Lambert, Sven Kjellström, Alena Wiegandt, Ragna Peterson Wulff, Cecilia Månsson, Gudrun Rutsdottir, Cecilia Emanuelsson |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Public Library of Science (PLoS)
2012
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Materias: | |
Acceso en línea: | https://doaj.org/article/42df66698a444a6b81c119185324f8d0 |
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