Circulating p16-Positive and p16-Negative Tumor Cells Serve as Independent Prognostic Indicators of Survival in Patients with Head and Neck Squamous Cell Carcinomas

Background: Decisions regarding the staging, prognosis, and treatment of patients with head and neck squamous cell carcinomas (HNSCCs) are made after determining their p16 expression levels and human papillomavirus (HPV) infection status. Methods: We investigated the prognostic roles of p16-positive...

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Autores principales:	Pei-Hung Chang, Hung-Ming Wang, Yung-Chia Kuo, Li-Yu Lee, Chia-Jung Liao, Hsuan-Chih Kuo, Cheng-Lung Hsu, Chun-Ta Liao, Sanger Hung-Chi Lin, Pei-Wei Huang, Tyler Min-Hsien Wu, Jason Chia-Hsun Hsieh
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	circulating tumor cells p16 expression head and neck squamous cell carcinoma HPV genotyping biomarker liquid biopsy Medicine R
Acceso en línea:	https://doaj.org/article/42e37b72e2c544d787bb5a3b2475e529
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spelling	oai:doaj.org-article:42e37b72e2c544d787bb5a3b2475e5292021-11-25T18:07:38ZCirculating p16-Positive and p16-Negative Tumor Cells Serve as Independent Prognostic Indicators of Survival in Patients with Head and Neck Squamous Cell Carcinomas10.3390/jpm111111562075-4426https://doaj.org/article/42e37b72e2c544d787bb5a3b2475e5292021-11-01T00:00:00Zhttps://www.mdpi.com/2075-4426/11/11/1156https://doaj.org/toc/2075-4426Background: Decisions regarding the staging, prognosis, and treatment of patients with head and neck squamous cell carcinomas (HNSCCs) are made after determining their p16 expression levels and human papillomavirus (HPV) infection status. Methods: We investigated the prognostic roles of p16-positive and p16-negative circulating tumor cells (CTCs) and their cell counts in HNSCC patients. We enrolled patients with locally advanced HNSCCs who received definitive concurrent chemoradiotherapy for final analysis. We performed CTC testing and p16 expression analysis before chemoradiotherapy. We analyzed the correlation between p16-positive and p16-negative CTCs and HPV genotyping, tissue p16 expression status, response to chemoradiotherapy, disease-free survival, and overall survival. Results: Forty-one patients who fulfilled the study criteria were prospectively enrolled for final analysis. The detection rates of p16-positive (>0 cells/mL blood) and p16-negative (≥3 cells/mL blood) CTCs were 51.2% (<i>n</i> = 21/41) and 70.7%, respectively. The best responses of chemoradiotherapy and the p16 positivity of CTCs are independent prognostic factors of disease progression, with hazard ratios of 1.738 (95% confidence interval (CI): 1.031–2.927), 5.497 (95% CI: 1.818–16.615), and 0.176 (95% CI: 0.056–0.554), respectively. The p16 positivity of CTCs was a prognostic factor for cancer death, with a hazard ratio of 0.294 (95% CI: 0.102–0.852). Conclusions: The p16-positive and p16-negative CTCs could predict outcomes in HNSCC patients receiving definitive chemoradiotherapy. This non-invasive CTC test could help stratify the risk and prognosis before chemoradiotherapy in clinical practice and enable us to perform de-intensifying therapies.Pei-Hung ChangHung-Ming WangYung-Chia KuoLi-Yu LeeChia-Jung LiaoHsuan-Chih KuoCheng-Lung HsuChun-Ta LiaoSanger Hung-Chi LinPei-Wei HuangTyler Min-Hsien WuJason Chia-Hsun HsiehMDPI AGarticlecirculating tumor cellsp16 expressionhead and neck squamous cell carcinomaHPV genotypingbiomarkerliquid biopsyMedicineRENJournal of Personalized Medicine, Vol 11, Iss 1156, p 1156 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	circulating tumor cells p16 expression head and neck squamous cell carcinoma HPV genotyping biomarker liquid biopsy Medicine R
spellingShingle	circulating tumor cells p16 expression head and neck squamous cell carcinoma HPV genotyping biomarker liquid biopsy Medicine R Pei-Hung Chang Hung-Ming Wang Yung-Chia Kuo Li-Yu Lee Chia-Jung Liao Hsuan-Chih Kuo Cheng-Lung Hsu Chun-Ta Liao Sanger Hung-Chi Lin Pei-Wei Huang Tyler Min-Hsien Wu Jason Chia-Hsun Hsieh Circulating p16-Positive and p16-Negative Tumor Cells Serve as Independent Prognostic Indicators of Survival in Patients with Head and Neck Squamous Cell Carcinomas
description	Background: Decisions regarding the staging, prognosis, and treatment of patients with head and neck squamous cell carcinomas (HNSCCs) are made after determining their p16 expression levels and human papillomavirus (HPV) infection status. Methods: We investigated the prognostic roles of p16-positive and p16-negative circulating tumor cells (CTCs) and their cell counts in HNSCC patients. We enrolled patients with locally advanced HNSCCs who received definitive concurrent chemoradiotherapy for final analysis. We performed CTC testing and p16 expression analysis before chemoradiotherapy. We analyzed the correlation between p16-positive and p16-negative CTCs and HPV genotyping, tissue p16 expression status, response to chemoradiotherapy, disease-free survival, and overall survival. Results: Forty-one patients who fulfilled the study criteria were prospectively enrolled for final analysis. The detection rates of p16-positive (>0 cells/mL blood) and p16-negative (≥3 cells/mL blood) CTCs were 51.2% (<i>n</i> = 21/41) and 70.7%, respectively. The best responses of chemoradiotherapy and the p16 positivity of CTCs are independent prognostic factors of disease progression, with hazard ratios of 1.738 (95% confidence interval (CI): 1.031–2.927), 5.497 (95% CI: 1.818–16.615), and 0.176 (95% CI: 0.056–0.554), respectively. The p16 positivity of CTCs was a prognostic factor for cancer death, with a hazard ratio of 0.294 (95% CI: 0.102–0.852). Conclusions: The p16-positive and p16-negative CTCs could predict outcomes in HNSCC patients receiving definitive chemoradiotherapy. This non-invasive CTC test could help stratify the risk and prognosis before chemoradiotherapy in clinical practice and enable us to perform de-intensifying therapies.
format	article
author	Pei-Hung Chang Hung-Ming Wang Yung-Chia Kuo Li-Yu Lee Chia-Jung Liao Hsuan-Chih Kuo Cheng-Lung Hsu Chun-Ta Liao Sanger Hung-Chi Lin Pei-Wei Huang Tyler Min-Hsien Wu Jason Chia-Hsun Hsieh
author_facet	Pei-Hung Chang Hung-Ming Wang Yung-Chia Kuo Li-Yu Lee Chia-Jung Liao Hsuan-Chih Kuo Cheng-Lung Hsu Chun-Ta Liao Sanger Hung-Chi Lin Pei-Wei Huang Tyler Min-Hsien Wu Jason Chia-Hsun Hsieh
author_sort	Pei-Hung Chang
title	Circulating p16-Positive and p16-Negative Tumor Cells Serve as Independent Prognostic Indicators of Survival in Patients with Head and Neck Squamous Cell Carcinomas
title_short	Circulating p16-Positive and p16-Negative Tumor Cells Serve as Independent Prognostic Indicators of Survival in Patients with Head and Neck Squamous Cell Carcinomas
title_full	Circulating p16-Positive and p16-Negative Tumor Cells Serve as Independent Prognostic Indicators of Survival in Patients with Head and Neck Squamous Cell Carcinomas
title_fullStr	Circulating p16-Positive and p16-Negative Tumor Cells Serve as Independent Prognostic Indicators of Survival in Patients with Head and Neck Squamous Cell Carcinomas
title_full_unstemmed	Circulating p16-Positive and p16-Negative Tumor Cells Serve as Independent Prognostic Indicators of Survival in Patients with Head and Neck Squamous Cell Carcinomas
title_sort	circulating p16-positive and p16-negative tumor cells serve as independent prognostic indicators of survival in patients with head and neck squamous cell carcinomas
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/42e37b72e2c544d787bb5a3b2475e529
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Circulating p16-Positive and p16-Negative Tumor Cells Serve as Independent Prognostic Indicators of Survival in Patients with Head and Neck Squamous Cell Carcinomas

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