Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination

Direct cloning of biosynthetic gene clusters is difficult due to their size and repetitive nature. Here, the authors present CAPTURE, which uses Cas12a and in vivo Cre-lox recombination for efficient cloning of gene clusters up to 113 kb, and demonstrate how this method can be used for large-scale d...

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Autores principales:	Behnam Enghiad, Chunshuai Huang, Fang Guo, Guangde Jiang, Bin Wang, S. Kasra Tabatabaei, Teresa A. Martin, Huimin Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/42e3dde80df44f6a95cfb8762a0ff62b
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spelling	oai:doaj.org-article:42e3dde80df44f6a95cfb8762a0ff62b2021-12-02T10:54:04ZCas12a-assisted precise targeted cloning using in vivo Cre-lox recombination10.1038/s41467-021-21275-42041-1723https://doaj.org/article/42e3dde80df44f6a95cfb8762a0ff62b2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-21275-4https://doaj.org/toc/2041-1723Direct cloning of biosynthetic gene clusters is difficult due to their size and repetitive nature. Here, the authors present CAPTURE, which uses Cas12a and in vivo Cre-lox recombination for efficient cloning of gene clusters up to 113 kb, and demonstrate how this method can be used for large-scale discovery of novel natural products.Behnam EnghiadChunshuai HuangFang GuoGuangde JiangBin WangS. Kasra TabatabaeiTeresa A. MartinHuimin ZhaoNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Behnam Enghiad Chunshuai Huang Fang Guo Guangde Jiang Bin Wang S. Kasra Tabatabaei Teresa A. Martin Huimin Zhao Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination
description	Direct cloning of biosynthetic gene clusters is difficult due to their size and repetitive nature. Here, the authors present CAPTURE, which uses Cas12a and in vivo Cre-lox recombination for efficient cloning of gene clusters up to 113 kb, and demonstrate how this method can be used for large-scale discovery of novel natural products.
format	article
author	Behnam Enghiad Chunshuai Huang Fang Guo Guangde Jiang Bin Wang S. Kasra Tabatabaei Teresa A. Martin Huimin Zhao
author_facet	Behnam Enghiad Chunshuai Huang Fang Guo Guangde Jiang Bin Wang S. Kasra Tabatabaei Teresa A. Martin Huimin Zhao
author_sort	Behnam Enghiad
title	Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination
title_short	Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination
title_full	Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination
title_fullStr	Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination
title_full_unstemmed	Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination
title_sort	cas12a-assisted precise targeted cloning using in vivo cre-lox recombination
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/42e3dde80df44f6a95cfb8762a0ff62b
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Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination

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