Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination
Direct cloning of biosynthetic gene clusters is difficult due to their size and repetitive nature. Here, the authors present CAPTURE, which uses Cas12a and in vivo Cre-lox recombination for efficient cloning of gene clusters up to 113 kb, and demonstrate how this method can be used for large-scale d...
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Nature Portfolio
2021
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oai:doaj.org-article:42e3dde80df44f6a95cfb8762a0ff62b2021-12-02T10:54:04ZCas12a-assisted precise targeted cloning using in vivo Cre-lox recombination10.1038/s41467-021-21275-42041-1723https://doaj.org/article/42e3dde80df44f6a95cfb8762a0ff62b2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-21275-4https://doaj.org/toc/2041-1723Direct cloning of biosynthetic gene clusters is difficult due to their size and repetitive nature. Here, the authors present CAPTURE, which uses Cas12a and in vivo Cre-lox recombination for efficient cloning of gene clusters up to 113 kb, and demonstrate how this method can be used for large-scale discovery of novel natural products.Behnam EnghiadChunshuai HuangFang GuoGuangde JiangBin WangS. Kasra TabatabaeiTeresa A. MartinHuimin ZhaoNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-11 (2021) |
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Science Q Behnam Enghiad Chunshuai Huang Fang Guo Guangde Jiang Bin Wang S. Kasra Tabatabaei Teresa A. Martin Huimin Zhao Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination |
| description |
Direct cloning of biosynthetic gene clusters is difficult due to their size and repetitive nature. Here, the authors present CAPTURE, which uses Cas12a and in vivo Cre-lox recombination for efficient cloning of gene clusters up to 113 kb, and demonstrate how this method can be used for large-scale discovery of novel natural products. |
| format |
article |
| author |
Behnam Enghiad Chunshuai Huang Fang Guo Guangde Jiang Bin Wang S. Kasra Tabatabaei Teresa A. Martin Huimin Zhao |
| author_facet |
Behnam Enghiad Chunshuai Huang Fang Guo Guangde Jiang Bin Wang S. Kasra Tabatabaei Teresa A. Martin Huimin Zhao |
| author_sort |
Behnam Enghiad |
| title |
Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination |
| title_short |
Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination |
| title_full |
Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination |
| title_fullStr |
Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination |
| title_full_unstemmed |
Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination |
| title_sort |
cas12a-assisted precise targeted cloning using in vivo cre-lox recombination |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/42e3dde80df44f6a95cfb8762a0ff62b |
| work_keys_str_mv |
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