The first clawed lobster virus Homarus gammarus nudivirus (HgNV n. sp.) expands the diversity of the Nudiviridae

Abstract Viral diseases of crustaceans are increasingly recognised as challenges to shellfish farms and fisheries. Here we describe the first naturally-occurring virus reported in any clawed lobster species. Hypertrophied nuclei with emarginated chromatin, characteristic histopathological lesions of...

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Autores principales:	Corey C. Holt, Michelle Stone, David Bass, Kelly S. Bateman, Ronny van Aerle, Carly L. Daniels, Mark van der Giezen, Stuart H. Ross, Chantelle Hooper, Grant D. Stentiford
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/42e57a828ebd4c5d88a67ffd3dddb29d
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spelling	oai:doaj.org-article:42e57a828ebd4c5d88a67ffd3dddb29d2021-12-02T15:09:29ZThe first clawed lobster virus Homarus gammarus nudivirus (HgNV n. sp.) expands the diversity of the Nudiviridae10.1038/s41598-019-46008-y2045-2322https://doaj.org/article/42e57a828ebd4c5d88a67ffd3dddb29d2019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-46008-yhttps://doaj.org/toc/2045-2322Abstract Viral diseases of crustaceans are increasingly recognised as challenges to shellfish farms and fisheries. Here we describe the first naturally-occurring virus reported in any clawed lobster species. Hypertrophied nuclei with emarginated chromatin, characteristic histopathological lesions of DNA virus infection, were observed within the hepatopancreatic epithelial cells of juvenile European lobsters (Homarus gammarus). Transmission electron microscopy revealed infection with a bacilliform virus containing a rod shaped nucleocapsid enveloped in an elliptical membrane. Assembly of PCR-free shotgun metagenomic sequencing produced a circular genome of 107,063 bp containing 97 open reading frames, the majority of which share sequence similarity with a virus infecting the black tiger shrimp: Penaeus monodon nudivirus (PmNV). Multiple phylogenetic analyses confirm the new virus to be a novel member of the Nudiviridae: Homarus gammarus nudivirus (HgNV). Evidence of occlusion body formation, characteristic of PmNV and its closest relatives, was not observed, questioning the horizontal transmission strategy of HgNV outside of the host. We discuss the potential impacts of HgNV on juvenile lobster growth and mortality and present HgNV-specific primers to serve as a diagnostic tool for monitoring the virus in wild and farmed lobster stocks.Corey C. HoltMichelle StoneDavid BassKelly S. BatemanRonny van AerleCarly L. DanielsMark van der GiezenStuart H. RossChantelle HooperGrant D. StentifordNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-15 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Corey C. Holt Michelle Stone David Bass Kelly S. Bateman Ronny van Aerle Carly L. Daniels Mark van der Giezen Stuart H. Ross Chantelle Hooper Grant D. Stentiford The first clawed lobster virus Homarus gammarus nudivirus (HgNV n. sp.) expands the diversity of the Nudiviridae
description	Abstract Viral diseases of crustaceans are increasingly recognised as challenges to shellfish farms and fisheries. Here we describe the first naturally-occurring virus reported in any clawed lobster species. Hypertrophied nuclei with emarginated chromatin, characteristic histopathological lesions of DNA virus infection, were observed within the hepatopancreatic epithelial cells of juvenile European lobsters (Homarus gammarus). Transmission electron microscopy revealed infection with a bacilliform virus containing a rod shaped nucleocapsid enveloped in an elliptical membrane. Assembly of PCR-free shotgun metagenomic sequencing produced a circular genome of 107,063 bp containing 97 open reading frames, the majority of which share sequence similarity with a virus infecting the black tiger shrimp: Penaeus monodon nudivirus (PmNV). Multiple phylogenetic analyses confirm the new virus to be a novel member of the Nudiviridae: Homarus gammarus nudivirus (HgNV). Evidence of occlusion body formation, characteristic of PmNV and its closest relatives, was not observed, questioning the horizontal transmission strategy of HgNV outside of the host. We discuss the potential impacts of HgNV on juvenile lobster growth and mortality and present HgNV-specific primers to serve as a diagnostic tool for monitoring the virus in wild and farmed lobster stocks.
format	article
author	Corey C. Holt Michelle Stone David Bass Kelly S. Bateman Ronny van Aerle Carly L. Daniels Mark van der Giezen Stuart H. Ross Chantelle Hooper Grant D. Stentiford
author_facet	Corey C. Holt Michelle Stone David Bass Kelly S. Bateman Ronny van Aerle Carly L. Daniels Mark van der Giezen Stuart H. Ross Chantelle Hooper Grant D. Stentiford
author_sort	Corey C. Holt
title	The first clawed lobster virus Homarus gammarus nudivirus (HgNV n. sp.) expands the diversity of the Nudiviridae
title_short	The first clawed lobster virus Homarus gammarus nudivirus (HgNV n. sp.) expands the diversity of the Nudiviridae
title_full	The first clawed lobster virus Homarus gammarus nudivirus (HgNV n. sp.) expands the diversity of the Nudiviridae
title_fullStr	The first clawed lobster virus Homarus gammarus nudivirus (HgNV n. sp.) expands the diversity of the Nudiviridae
title_full_unstemmed	The first clawed lobster virus Homarus gammarus nudivirus (HgNV n. sp.) expands the diversity of the Nudiviridae
title_sort	first clawed lobster virus homarus gammarus nudivirus (hgnv n. sp.) expands the diversity of the nudiviridae
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/42e57a828ebd4c5d88a67ffd3dddb29d
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The first clawed lobster virus Homarus gammarus nudivirus (HgNV n. sp.) expands the diversity of the Nudiviridae

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