Aqueous Two-Phase System–Ion Chromatography for Determination of Thiocyanate in Raw Milk

Thiocyanate could effectively inhibit bacteria in milk and extend the shelf life of milk. However, excessive addition will lead to health risks. Therefore, the determination of thiocyanate in raw milk has received a lot of attention, but the determination could be interfered with by other components...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bin Jiang, Shaojing Zhong, Hongliang Yu, Peifeng Chen, Baoyun Li, Dongmei Li, Chunhong Liu, Zhibiao Feng, Bo Tian
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	thiocyanate raw milk aqueous two-phase system ion chromatography extraction Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/42eb3e0308c14b10b594e326c33eb25c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:42eb3e0308c14b10b594e326c33eb25c
record_format	dspace
spelling	oai:doaj.org-article:42eb3e0308c14b10b594e326c33eb25c2021-11-25T18:59:24ZAqueous Two-Phase System–Ion Chromatography for Determination of Thiocyanate in Raw Milk10.3390/separations81102122297-8739https://doaj.org/article/42eb3e0308c14b10b594e326c33eb25c2021-11-01T00:00:00Zhttps://www.mdpi.com/2297-8739/8/11/212https://doaj.org/toc/2297-8739Thiocyanate could effectively inhibit bacteria in milk and extend the shelf life of milk. However, excessive addition will lead to health risks. Therefore, the determination of thiocyanate in raw milk has received a lot of attention, but the determination could be interfered with by other components in raw milk and the pre-treatment of raw milk is complex. In this study, a new pretreatment method combined with ion chromatography (IC) for rapid and sensitive determination of thiocyanate is proposed. An acetonitrile/(NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> aqueous two-phase system (ATPS) was developed for the separation and enrichment of thiocyanate in raw milk. Response surface methodology was performed to optimize the extraction conditions and an efficient pretreatment were obtained using ATPS composed of 42% acetonitrile (<i>w</i>/<i>w</i>) and 16% (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> (<i>w</i>/<i>w</i>), with the pH 4.7, and the recovery of thiocyanate reached 107.24 ± 0.5%, and the enrichment ratio was 10.74 ± 0.03. IC was used to establish a thiocyanate enrichment method. The linear range was from 0.05 to 15 mg/L and R<sup>2</sup> = 0.998, the limit of detection (LOD) was 0.2 μg/L, the limit of quantification (LQD) was 0.6 μg/L. Hence, it is feasible to combine ATPS with IC for the enrichment and determination of thiocyanate in raw milk.Bin JiangShaojing ZhongHongliang YuPeifeng ChenBaoyun LiDongmei LiChunhong LiuZhibiao FengBo TianMDPI AGarticlethiocyanateraw milkaqueous two-phase systemion chromatographyextractionPhysicsQC1-999ChemistryQD1-999ENSeparations, Vol 8, Iss 212, p 212 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	thiocyanate raw milk aqueous two-phase system ion chromatography extraction Physics QC1-999 Chemistry QD1-999
spellingShingle	thiocyanate raw milk aqueous two-phase system ion chromatography extraction Physics QC1-999 Chemistry QD1-999 Bin Jiang Shaojing Zhong Hongliang Yu Peifeng Chen Baoyun Li Dongmei Li Chunhong Liu Zhibiao Feng Bo Tian Aqueous Two-Phase System–Ion Chromatography for Determination of Thiocyanate in Raw Milk
description	Thiocyanate could effectively inhibit bacteria in milk and extend the shelf life of milk. However, excessive addition will lead to health risks. Therefore, the determination of thiocyanate in raw milk has received a lot of attention, but the determination could be interfered with by other components in raw milk and the pre-treatment of raw milk is complex. In this study, a new pretreatment method combined with ion chromatography (IC) for rapid and sensitive determination of thiocyanate is proposed. An acetonitrile/(NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> aqueous two-phase system (ATPS) was developed for the separation and enrichment of thiocyanate in raw milk. Response surface methodology was performed to optimize the extraction conditions and an efficient pretreatment were obtained using ATPS composed of 42% acetonitrile (<i>w</i>/<i>w</i>) and 16% (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> (<i>w</i>/<i>w</i>), with the pH 4.7, and the recovery of thiocyanate reached 107.24 ± 0.5%, and the enrichment ratio was 10.74 ± 0.03. IC was used to establish a thiocyanate enrichment method. The linear range was from 0.05 to 15 mg/L and R<sup>2</sup> = 0.998, the limit of detection (LOD) was 0.2 μg/L, the limit of quantification (LQD) was 0.6 μg/L. Hence, it is feasible to combine ATPS with IC for the enrichment and determination of thiocyanate in raw milk.
format	article
author	Bin Jiang Shaojing Zhong Hongliang Yu Peifeng Chen Baoyun Li Dongmei Li Chunhong Liu Zhibiao Feng Bo Tian
author_facet	Bin Jiang Shaojing Zhong Hongliang Yu Peifeng Chen Baoyun Li Dongmei Li Chunhong Liu Zhibiao Feng Bo Tian
author_sort	Bin Jiang
title	Aqueous Two-Phase System–Ion Chromatography for Determination of Thiocyanate in Raw Milk
title_short	Aqueous Two-Phase System–Ion Chromatography for Determination of Thiocyanate in Raw Milk
title_full	Aqueous Two-Phase System–Ion Chromatography for Determination of Thiocyanate in Raw Milk
title_fullStr	Aqueous Two-Phase System–Ion Chromatography for Determination of Thiocyanate in Raw Milk
title_full_unstemmed	Aqueous Two-Phase System–Ion Chromatography for Determination of Thiocyanate in Raw Milk
title_sort	aqueous two-phase system–ion chromatography for determination of thiocyanate in raw milk
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/42eb3e0308c14b10b594e326c33eb25c
work_keys_str_mv	AT binjiang aqueoustwophasesystemionchromatographyfordeterminationofthiocyanateinrawmilk AT shaojingzhong aqueoustwophasesystemionchromatographyfordeterminationofthiocyanateinrawmilk AT hongliangyu aqueoustwophasesystemionchromatographyfordeterminationofthiocyanateinrawmilk AT peifengchen aqueoustwophasesystemionchromatographyfordeterminationofthiocyanateinrawmilk AT baoyunli aqueoustwophasesystemionchromatographyfordeterminationofthiocyanateinrawmilk AT dongmeili aqueoustwophasesystemionchromatographyfordeterminationofthiocyanateinrawmilk AT chunhongliu aqueoustwophasesystemionchromatographyfordeterminationofthiocyanateinrawmilk AT zhibiaofeng aqueoustwophasesystemionchromatographyfordeterminationofthiocyanateinrawmilk AT botian aqueoustwophasesystemionchromatographyfordeterminationofthiocyanateinrawmilk
_version_	1718410458522714112

Aqueous Two-Phase System–Ion Chromatography for Determination of Thiocyanate in Raw Milk

Ejemplares similares