Loading equine oocytes with cryoprotective agents captured with a finite element method model

Abstract Cryopreservation can be used to store equine oocytes for extended periods so that they can be used in artificial reproduction technologies at a desired time point. It requires use of cryoprotective agents (CPAs) to protect the oocytes against freezing injury. The intracellular introduction...

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Autores principales:	Sercan Içli, Meisam Soleimani, Harriëtte Oldenhof, Harald Sieme, Peter Wriggers, Willem F. Wolkers
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/42ec7603b6d742699dfadd8d7f2b2fb8
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spelling	oai:doaj.org-article:42ec7603b6d742699dfadd8d7f2b2fb82021-12-02T17:13:17ZLoading equine oocytes with cryoprotective agents captured with a finite element method model10.1038/s41598-021-99287-92045-2322https://doaj.org/article/42ec7603b6d742699dfadd8d7f2b2fb82021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99287-9https://doaj.org/toc/2045-2322Abstract Cryopreservation can be used to store equine oocytes for extended periods so that they can be used in artificial reproduction technologies at a desired time point. It requires use of cryoprotective agents (CPAs) to protect the oocytes against freezing injury. The intracellular introduction of CPAs, however, may cause irreversible osmotic damage. The response of cells exposed to CPA solutions is governed by the permeability of the cellular membrane towards water and the CPAs. In this study, a mathematical mass transport model describing the permeation of water and CPAs across an oocyte membrane was used to simulate oocyte volume responses and concomitant intracellular CPA concentrations during the exposure of oocytes to CPA solutions. The results of the analytical simulations were subsequently used to develop a phenomenological finite element method (FEM) continuum model to capture the response of oocytes exposed to CPA solutions with spatial information. FEM simulations were used to depict spatial differences in CPA concentration during CPA permeation, namely at locations near the membrane surface and towards the middle of the cell, and to capture corresponding changes in deformation and hydrostatic pressure. FEM simulations of the multiple processes occurring during CPA loading of oocytes are a valuable tool to increase our understanding of the mechanisms underlying cryopreservation outcome.Sercan IçliMeisam SoleimaniHarriëtte OldenhofHarald SiemePeter WriggersWillem F. WolkersNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sercan Içli Meisam Soleimani Harriëtte Oldenhof Harald Sieme Peter Wriggers Willem F. Wolkers Loading equine oocytes with cryoprotective agents captured with a finite element method model
description	Abstract Cryopreservation can be used to store equine oocytes for extended periods so that they can be used in artificial reproduction technologies at a desired time point. It requires use of cryoprotective agents (CPAs) to protect the oocytes against freezing injury. The intracellular introduction of CPAs, however, may cause irreversible osmotic damage. The response of cells exposed to CPA solutions is governed by the permeability of the cellular membrane towards water and the CPAs. In this study, a mathematical mass transport model describing the permeation of water and CPAs across an oocyte membrane was used to simulate oocyte volume responses and concomitant intracellular CPA concentrations during the exposure of oocytes to CPA solutions. The results of the analytical simulations were subsequently used to develop a phenomenological finite element method (FEM) continuum model to capture the response of oocytes exposed to CPA solutions with spatial information. FEM simulations were used to depict spatial differences in CPA concentration during CPA permeation, namely at locations near the membrane surface and towards the middle of the cell, and to capture corresponding changes in deformation and hydrostatic pressure. FEM simulations of the multiple processes occurring during CPA loading of oocytes are a valuable tool to increase our understanding of the mechanisms underlying cryopreservation outcome.
format	article
author	Sercan Içli Meisam Soleimani Harriëtte Oldenhof Harald Sieme Peter Wriggers Willem F. Wolkers
author_facet	Sercan Içli Meisam Soleimani Harriëtte Oldenhof Harald Sieme Peter Wriggers Willem F. Wolkers
author_sort	Sercan Içli
title	Loading equine oocytes with cryoprotective agents captured with a finite element method model
title_short	Loading equine oocytes with cryoprotective agents captured with a finite element method model
title_full	Loading equine oocytes with cryoprotective agents captured with a finite element method model
title_fullStr	Loading equine oocytes with cryoprotective agents captured with a finite element method model
title_full_unstemmed	Loading equine oocytes with cryoprotective agents captured with a finite element method model
title_sort	loading equine oocytes with cryoprotective agents captured with a finite element method model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/42ec7603b6d742699dfadd8d7f2b2fb8
work_keys_str_mv	AT sercanicli loadingequineoocyteswithcryoprotectiveagentscapturedwithafiniteelementmethodmodel AT meisamsoleimani loadingequineoocyteswithcryoprotectiveagentscapturedwithafiniteelementmethodmodel AT harrietteoldenhof loadingequineoocyteswithcryoprotectiveagentscapturedwithafiniteelementmethodmodel AT haraldsieme loadingequineoocyteswithcryoprotectiveagentscapturedwithafiniteelementmethodmodel AT peterwriggers loadingequineoocyteswithcryoprotectiveagentscapturedwithafiniteelementmethodmodel AT willemfwolkers loadingequineoocyteswithcryoprotectiveagentscapturedwithafiniteelementmethodmodel
_version_	1718381373555736576

Loading equine oocytes with cryoprotective agents captured with a finite element method model

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