Vesicular Stomatitis Virus-Based Vaccine Protects Mice against Crimean-Congo Hemorrhagic Fever

Abstract Crimean-Congo hemorrhagic fever virus (CCHFV), a tick-borne bunyavirus, can cause a life-threatening hemorrhagic syndrome in humans but not in its animal host. The virus is widely distributed throughout southeastern Europe, the Middle East, Africa, and Asia. Disease management has proven di...

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Autores principales:	Sergio E. Rodriguez, Robert W. Cross, Karla A. Fenton, Dennis A. Bente, Chad E. Mire, Thomas W. Geisbert
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/42f72c4d97ec490a8d99bd24eb14ed8f
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spelling	oai:doaj.org-article:42f72c4d97ec490a8d99bd24eb14ed8f2021-12-02T15:09:39ZVesicular Stomatitis Virus-Based Vaccine Protects Mice against Crimean-Congo Hemorrhagic Fever10.1038/s41598-019-44210-62045-2322https://doaj.org/article/42f72c4d97ec490a8d99bd24eb14ed8f2019-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-44210-6https://doaj.org/toc/2045-2322Abstract Crimean-Congo hemorrhagic fever virus (CCHFV), a tick-borne bunyavirus, can cause a life-threatening hemorrhagic syndrome in humans but not in its animal host. The virus is widely distributed throughout southeastern Europe, the Middle East, Africa, and Asia. Disease management has proven difficult and there are no broadly licensed vaccines or therapeutics. Recombinant vesicular stomatitis viruses (rVSV) expressing foreign glycoproteins (GP) have shown promise as experimental vaccines for several viral hemorrhagic fevers. Here, we developed and assessed a replication competent rVSV vector expressing the CCHFV glycoprotein precursor (GPC), which encodes CCHFV structural glycoproteins. This construct drives strong expression of CCHFV-GP, in vitro. Using these vectors, we vaccinated STAT-1 knock-out mice, an animal model for CCHFV. The vector was tolerated and 100% efficacious against challenge from a clinical strain of CCHFV. Anti-CCHFV-GP IgG and neutralizing antibody titers were observed in surviving animals. This study demonstrates that a rVSV expressing only the CCHFV-GP has the potential to serve as a replication competent vaccine platform against CCHF infections.Sergio E. RodriguezRobert W. CrossKarla A. FentonDennis A. BenteChad E. MireThomas W. GeisbertNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sergio E. Rodriguez Robert W. Cross Karla A. Fenton Dennis A. Bente Chad E. Mire Thomas W. Geisbert Vesicular Stomatitis Virus-Based Vaccine Protects Mice against Crimean-Congo Hemorrhagic Fever
description	Abstract Crimean-Congo hemorrhagic fever virus (CCHFV), a tick-borne bunyavirus, can cause a life-threatening hemorrhagic syndrome in humans but not in its animal host. The virus is widely distributed throughout southeastern Europe, the Middle East, Africa, and Asia. Disease management has proven difficult and there are no broadly licensed vaccines or therapeutics. Recombinant vesicular stomatitis viruses (rVSV) expressing foreign glycoproteins (GP) have shown promise as experimental vaccines for several viral hemorrhagic fevers. Here, we developed and assessed a replication competent rVSV vector expressing the CCHFV glycoprotein precursor (GPC), which encodes CCHFV structural glycoproteins. This construct drives strong expression of CCHFV-GP, in vitro. Using these vectors, we vaccinated STAT-1 knock-out mice, an animal model for CCHFV. The vector was tolerated and 100% efficacious against challenge from a clinical strain of CCHFV. Anti-CCHFV-GP IgG and neutralizing antibody titers were observed in surviving animals. This study demonstrates that a rVSV expressing only the CCHFV-GP has the potential to serve as a replication competent vaccine platform against CCHF infections.
format	article
author	Sergio E. Rodriguez Robert W. Cross Karla A. Fenton Dennis A. Bente Chad E. Mire Thomas W. Geisbert
author_facet	Sergio E. Rodriguez Robert W. Cross Karla A. Fenton Dennis A. Bente Chad E. Mire Thomas W. Geisbert
author_sort	Sergio E. Rodriguez
title	Vesicular Stomatitis Virus-Based Vaccine Protects Mice against Crimean-Congo Hemorrhagic Fever
title_short	Vesicular Stomatitis Virus-Based Vaccine Protects Mice against Crimean-Congo Hemorrhagic Fever
title_full	Vesicular Stomatitis Virus-Based Vaccine Protects Mice against Crimean-Congo Hemorrhagic Fever
title_fullStr	Vesicular Stomatitis Virus-Based Vaccine Protects Mice against Crimean-Congo Hemorrhagic Fever
title_full_unstemmed	Vesicular Stomatitis Virus-Based Vaccine Protects Mice against Crimean-Congo Hemorrhagic Fever
title_sort	vesicular stomatitis virus-based vaccine protects mice against crimean-congo hemorrhagic fever
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/42f72c4d97ec490a8d99bd24eb14ed8f
work_keys_str_mv	AT sergioerodriguez vesicularstomatitisvirusbasedvaccineprotectsmiceagainstcrimeancongohemorrhagicfever AT robertwcross vesicularstomatitisvirusbasedvaccineprotectsmiceagainstcrimeancongohemorrhagicfever AT karlaafenton vesicularstomatitisvirusbasedvaccineprotectsmiceagainstcrimeancongohemorrhagicfever AT dennisabente vesicularstomatitisvirusbasedvaccineprotectsmiceagainstcrimeancongohemorrhagicfever AT chademire vesicularstomatitisvirusbasedvaccineprotectsmiceagainstcrimeancongohemorrhagicfever AT thomaswgeisbert vesicularstomatitisvirusbasedvaccineprotectsmiceagainstcrimeancongohemorrhagicfever
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Vesicular Stomatitis Virus-Based Vaccine Protects Mice against Crimean-Congo Hemorrhagic Fever

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