The effect of biologically active compounds in the mucus of slugs Limax maximus and Arion rufus on human skin cells

Abstract Molluscs are one of the sources of biologically active substances, which are now intensively studied, especially for their anti-cancer properties. Malignant melanoma originates from melanocytes, develops very quickly and is associated with poor prognosis. Therefore, the aim of the study was...

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Autores principales:	Leśków Anna, Tarnowska Małgorzata, Szczuka Izabela, Diakowska Dorota
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/42fc55ed33424a56ad03a78ef88feb3b
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spelling	oai:doaj.org-article:42fc55ed33424a56ad03a78ef88feb3b2021-12-02T18:48:23ZThe effect of biologically active compounds in the mucus of slugs Limax maximus and Arion rufus on human skin cells10.1038/s41598-021-98183-62045-2322https://doaj.org/article/42fc55ed33424a56ad03a78ef88feb3b2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98183-6https://doaj.org/toc/2045-2322Abstract Molluscs are one of the sources of biologically active substances, which are now intensively studied, especially for their anti-cancer properties. Malignant melanoma originates from melanocytes, develops very quickly and is associated with poor prognosis. Therefore, the aim of the study was to assess the properties of biologically active compounds in sterile mucus isolated from slugs Limax maximus and Arion rufus. Tested mucus were isolated using the new self-developed method which is safe for the environment and the animal donors. The impact of the mucus on human keratinocytes CCD 1106 KERTr and malignant melanoma cells A-375 was examined using MTT assay and SRB assay, which allowed us to determine the cell metabolic activity and cell number after treating them with slug mucus isolated from Limax maximus and Arion rufus decreased human keratinocytes and melanoma cells metabolic activity as well as manifested properties of reducing the number of cells in both tested cell lines, and therefore can be a source of biologically active substances with anticancer potential.Leśków AnnaTarnowska MałgorzataSzczuka IzabelaDiakowska DorotaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Leśków Anna Tarnowska Małgorzata Szczuka Izabela Diakowska Dorota The effect of biologically active compounds in the mucus of slugs Limax maximus and Arion rufus on human skin cells
description	Abstract Molluscs are one of the sources of biologically active substances, which are now intensively studied, especially for their anti-cancer properties. Malignant melanoma originates from melanocytes, develops very quickly and is associated with poor prognosis. Therefore, the aim of the study was to assess the properties of biologically active compounds in sterile mucus isolated from slugs Limax maximus and Arion rufus. Tested mucus were isolated using the new self-developed method which is safe for the environment and the animal donors. The impact of the mucus on human keratinocytes CCD 1106 KERTr and malignant melanoma cells A-375 was examined using MTT assay and SRB assay, which allowed us to determine the cell metabolic activity and cell number after treating them with slug mucus isolated from Limax maximus and Arion rufus decreased human keratinocytes and melanoma cells metabolic activity as well as manifested properties of reducing the number of cells in both tested cell lines, and therefore can be a source of biologically active substances with anticancer potential.
format	article
author	Leśków Anna Tarnowska Małgorzata Szczuka Izabela Diakowska Dorota
author_facet	Leśków Anna Tarnowska Małgorzata Szczuka Izabela Diakowska Dorota
author_sort	Leśków Anna
title	The effect of biologically active compounds in the mucus of slugs Limax maximus and Arion rufus on human skin cells
title_short	The effect of biologically active compounds in the mucus of slugs Limax maximus and Arion rufus on human skin cells
title_full	The effect of biologically active compounds in the mucus of slugs Limax maximus and Arion rufus on human skin cells
title_fullStr	The effect of biologically active compounds in the mucus of slugs Limax maximus and Arion rufus on human skin cells
title_full_unstemmed	The effect of biologically active compounds in the mucus of slugs Limax maximus and Arion rufus on human skin cells
title_sort	effect of biologically active compounds in the mucus of slugs limax maximus and arion rufus on human skin cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/42fc55ed33424a56ad03a78ef88feb3b
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The effect of biologically active compounds in the mucus of slugs Limax maximus and Arion rufus on human skin cells

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