The Effect of Hydroalcoholic Extract of Glaucium Flavum on the Activity of Anti Oxidative Enzymes in the Heart and Brain of Alloxan Induced Diabetic Rats

BACKGROUND AND OBJECTIVE: Oxidative stress is the connection between diabetes and neuropathies and micro vascular disorders. This study was designed to investigate the effect of hydroalcoholic extract of Glaucium Flavum on the tissue activity of antioxidant enzymes in the heart and brain of Alloxan...

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Autores principales: A Khoshvaghti, G H Darya, F Hashemi, M Klantari, K Hushmandi
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Publicado: Babol University of Medical Sciences 2019
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spelling oai:doaj.org-article:42fd0a743f634a478e009332bd5c09962021-11-10T08:30:37ZThe Effect of Hydroalcoholic Extract of Glaucium Flavum on the Activity of Anti Oxidative Enzymes in the Heart and Brain of Alloxan Induced Diabetic Rats1561-41072251-7170https://doaj.org/article/42fd0a743f634a478e009332bd5c09962019-12-01T00:00:00Zhttp://jbums.org/article-1-8596-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Oxidative stress is the connection between diabetes and neuropathies and micro vascular disorders. This study was designed to investigate the effect of hydroalcoholic extract of Glaucium Flavum on the tissue activity of antioxidant enzymes in the heart and brain of Alloxan induced diabetic rats. METHODS: In this experimental study, 32 male rats were randomly divided into four groups of eight including control, diabetic, diabetic rats treated by Glaucium Flavum with dose of 500mg/kg and diabetic rats treated with Glibenclamide 5μg/kg. Diabetes was induced by single injection of 120mg/kg of Alloxan. After one month, activity of SOD, CAT and GPX were measured in the heart and brain tissues and analyzed. FINDINGS: Cardiac activity of all three enzymes in the diabetic + extract group were significantly higher than diabetic control (p<0.001). Activity of SOD in brain had a significant difference in comparison to diabetic + extract (8.79±1.4) and diabetic +drug groups (6.77±1.7) (p=0.03). As the same, CAT activity in diabetic+extract group (4.64±1.2) was significantly higher than diabetic + drug group (3.83±1.5) (p<0.001). Similar to the two previous state, GPX activity in diabetic+extract group (4.23±0.7) was significantly higher than diabetic+drug group (3.64±0.4) (p=0.03). CONCLUSION: The present study declared that yellow Glaucium Flavum extract can promote the main protective enzymatic mechanisms against diabetic induced oxidative stress in heart and brain. In addition, the effect of the extract was more successful than the effect of Glibenclamide and this effect was more pronounced in brain tissue.A KhoshvaghtiG H DaryaF HashemiM KlantariK HushmandiBabol University of Medical Sciencesarticlediabetes mellitusglaucium flavumsuperoxide dismutasecatalaseglutathione peroxidasebrainheartratalloxanMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 21, Iss 1, Pp 331-337 (2019)
institution DOAJ
collection DOAJ
language EN
FA
topic diabetes mellitus
glaucium flavum
superoxide dismutase
catalase
glutathione peroxidase
brain
heart
rat
alloxan
Medicine
R
Medicine (General)
R5-920
spellingShingle diabetes mellitus
glaucium flavum
superoxide dismutase
catalase
glutathione peroxidase
brain
heart
rat
alloxan
Medicine
R
Medicine (General)
R5-920
A Khoshvaghti
G H Darya
F Hashemi
M Klantari
K Hushmandi
The Effect of Hydroalcoholic Extract of Glaucium Flavum on the Activity of Anti Oxidative Enzymes in the Heart and Brain of Alloxan Induced Diabetic Rats
description BACKGROUND AND OBJECTIVE: Oxidative stress is the connection between diabetes and neuropathies and micro vascular disorders. This study was designed to investigate the effect of hydroalcoholic extract of Glaucium Flavum on the tissue activity of antioxidant enzymes in the heart and brain of Alloxan induced diabetic rats. METHODS: In this experimental study, 32 male rats were randomly divided into four groups of eight including control, diabetic, diabetic rats treated by Glaucium Flavum with dose of 500mg/kg and diabetic rats treated with Glibenclamide 5μg/kg. Diabetes was induced by single injection of 120mg/kg of Alloxan. After one month, activity of SOD, CAT and GPX were measured in the heart and brain tissues and analyzed. FINDINGS: Cardiac activity of all three enzymes in the diabetic + extract group were significantly higher than diabetic control (p<0.001). Activity of SOD in brain had a significant difference in comparison to diabetic + extract (8.79±1.4) and diabetic +drug groups (6.77±1.7) (p=0.03). As the same, CAT activity in diabetic+extract group (4.64±1.2) was significantly higher than diabetic + drug group (3.83±1.5) (p<0.001). Similar to the two previous state, GPX activity in diabetic+extract group (4.23±0.7) was significantly higher than diabetic+drug group (3.64±0.4) (p=0.03). CONCLUSION: The present study declared that yellow Glaucium Flavum extract can promote the main protective enzymatic mechanisms against diabetic induced oxidative stress in heart and brain. In addition, the effect of the extract was more successful than the effect of Glibenclamide and this effect was more pronounced in brain tissue.
format article
author A Khoshvaghti
G H Darya
F Hashemi
M Klantari
K Hushmandi
author_facet A Khoshvaghti
G H Darya
F Hashemi
M Klantari
K Hushmandi
author_sort A Khoshvaghti
title The Effect of Hydroalcoholic Extract of Glaucium Flavum on the Activity of Anti Oxidative Enzymes in the Heart and Brain of Alloxan Induced Diabetic Rats
title_short The Effect of Hydroalcoholic Extract of Glaucium Flavum on the Activity of Anti Oxidative Enzymes in the Heart and Brain of Alloxan Induced Diabetic Rats
title_full The Effect of Hydroalcoholic Extract of Glaucium Flavum on the Activity of Anti Oxidative Enzymes in the Heart and Brain of Alloxan Induced Diabetic Rats
title_fullStr The Effect of Hydroalcoholic Extract of Glaucium Flavum on the Activity of Anti Oxidative Enzymes in the Heart and Brain of Alloxan Induced Diabetic Rats
title_full_unstemmed The Effect of Hydroalcoholic Extract of Glaucium Flavum on the Activity of Anti Oxidative Enzymes in the Heart and Brain of Alloxan Induced Diabetic Rats
title_sort effect of hydroalcoholic extract of glaucium flavum on the activity of anti oxidative enzymes in the heart and brain of alloxan induced diabetic rats
publisher Babol University of Medical Sciences
publishDate 2019
url https://doaj.org/article/42fd0a743f634a478e009332bd5c0996
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