Functional and structural characterization of osteocytic MLO-Y4 cell proteins encoded by genes differentially expressed in response to mechanical signals in vitro

Abstract The anabolic response of bone to mechanical load is partially the result of osteocyte response to fluid flow-induced shear stress. Understanding signaling pathways activated in osteocytes exposed to fluid flow could identify novel signaling pathways involved in the response of bone to mecha...

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Autores principales:	Fanchi Meng, Graeme F. Murray, Lukasz Kurgan, Henry J. Donahue
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/42fd3c6dc6bd456dbbd3642c713a3248
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spelling	oai:doaj.org-article:42fd3c6dc6bd456dbbd3642c713a32482021-12-02T11:41:25ZFunctional and structural characterization of osteocytic MLO-Y4 cell proteins encoded by genes differentially expressed in response to mechanical signals in vitro10.1038/s41598-018-25113-42045-2322https://doaj.org/article/42fd3c6dc6bd456dbbd3642c713a32482018-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25113-4https://doaj.org/toc/2045-2322Abstract The anabolic response of bone to mechanical load is partially the result of osteocyte response to fluid flow-induced shear stress. Understanding signaling pathways activated in osteocytes exposed to fluid flow could identify novel signaling pathways involved in the response of bone to mechanical load. Bioinformatics allows for a unique perspective and provides key first steps in understanding these signaling pathways. We examined proteins encoded by genes differentially expressed in response to fluid flow in murine osteocytic MLO-Y4 cells. We considered structural and functional characteristics including putative intrinsic disorder, evolutionary conservation, interconnectedness in protein-protein interaction networks, and cellular localization. Our analysis suggests that proteins encoded by fluid flow activated genes have lower than expected conservation, are depleted in intrinsic disorder, maintain typical levels of connectivity for the murine proteome, and are found in the cytoplasm and extracellular space. Pathway analyses reveal that these proteins are associated with cellular response to stress, chemokine and cytokine activity, enzyme binding, and osteoclast differentiation. The lower than expected disorder of proteins encoded by flow activated genes suggests they are relatively specialized.Fanchi MengGraeme F. MurrayLukasz KurganHenry J. DonahueNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fanchi Meng Graeme F. Murray Lukasz Kurgan Henry J. Donahue Functional and structural characterization of osteocytic MLO-Y4 cell proteins encoded by genes differentially expressed in response to mechanical signals in vitro
description	Abstract The anabolic response of bone to mechanical load is partially the result of osteocyte response to fluid flow-induced shear stress. Understanding signaling pathways activated in osteocytes exposed to fluid flow could identify novel signaling pathways involved in the response of bone to mechanical load. Bioinformatics allows for a unique perspective and provides key first steps in understanding these signaling pathways. We examined proteins encoded by genes differentially expressed in response to fluid flow in murine osteocytic MLO-Y4 cells. We considered structural and functional characteristics including putative intrinsic disorder, evolutionary conservation, interconnectedness in protein-protein interaction networks, and cellular localization. Our analysis suggests that proteins encoded by fluid flow activated genes have lower than expected conservation, are depleted in intrinsic disorder, maintain typical levels of connectivity for the murine proteome, and are found in the cytoplasm and extracellular space. Pathway analyses reveal that these proteins are associated with cellular response to stress, chemokine and cytokine activity, enzyme binding, and osteoclast differentiation. The lower than expected disorder of proteins encoded by flow activated genes suggests they are relatively specialized.
format	article
author	Fanchi Meng Graeme F. Murray Lukasz Kurgan Henry J. Donahue
author_facet	Fanchi Meng Graeme F. Murray Lukasz Kurgan Henry J. Donahue
author_sort	Fanchi Meng
title	Functional and structural characterization of osteocytic MLO-Y4 cell proteins encoded by genes differentially expressed in response to mechanical signals in vitro
title_short	Functional and structural characterization of osteocytic MLO-Y4 cell proteins encoded by genes differentially expressed in response to mechanical signals in vitro
title_full	Functional and structural characterization of osteocytic MLO-Y4 cell proteins encoded by genes differentially expressed in response to mechanical signals in vitro
title_fullStr	Functional and structural characterization of osteocytic MLO-Y4 cell proteins encoded by genes differentially expressed in response to mechanical signals in vitro
title_full_unstemmed	Functional and structural characterization of osteocytic MLO-Y4 cell proteins encoded by genes differentially expressed in response to mechanical signals in vitro
title_sort	functional and structural characterization of osteocytic mlo-y4 cell proteins encoded by genes differentially expressed in response to mechanical signals in vitro
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/42fd3c6dc6bd456dbbd3642c713a3248
work_keys_str_mv	AT fanchimeng functionalandstructuralcharacterizationofosteocyticmloy4cellproteinsencodedbygenesdifferentiallyexpressedinresponsetomechanicalsignalsinvitro AT graemefmurray functionalandstructuralcharacterizationofosteocyticmloy4cellproteinsencodedbygenesdifferentiallyexpressedinresponsetomechanicalsignalsinvitro AT lukaszkurgan functionalandstructuralcharacterizationofosteocyticmloy4cellproteinsencodedbygenesdifferentiallyexpressedinresponsetomechanicalsignalsinvitro AT henryjdonahue functionalandstructuralcharacterizationofosteocyticmloy4cellproteinsencodedbygenesdifferentiallyexpressedinresponsetomechanicalsignalsinvitro
_version_	1718395395136028672

Functional and structural characterization of osteocytic MLO-Y4 cell proteins encoded by genes differentially expressed in response to mechanical signals in vitro

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