Diurnal and circadian rhythms in the tomato transcriptome and their modulation by cryptochrome photoreceptors.

<h4>Background</h4>Circadian clocks are internal molecular time-keeping mechanisms that provide living organisms with the ability to adjust their growth and physiology and to anticipate diurnal environmental changes. Circadian clocks, without exception, respond to light and, in plants, l...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Paolo Facella, Loredana Lopez, Fabrizio Carbone, David W Galbraith, Giovanni Giuliano, Gaetano Perrotta
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/430764ee0e7e468097e4defce9b6c927
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:430764ee0e7e468097e4defce9b6c927
record_format	dspace
spelling	oai:doaj.org-article:430764ee0e7e468097e4defce9b6c9272021-11-25T06:11:24ZDiurnal and circadian rhythms in the tomato transcriptome and their modulation by cryptochrome photoreceptors.1932-620310.1371/journal.pone.0002798https://doaj.org/article/430764ee0e7e468097e4defce9b6c9272008-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18665253/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Circadian clocks are internal molecular time-keeping mechanisms that provide living organisms with the ability to adjust their growth and physiology and to anticipate diurnal environmental changes. Circadian clocks, without exception, respond to light and, in plants, light is the most potent and best characterized entraining stimulus. The capacity of plants to respond to light is achieved through a number of photo-perceptive proteins including cryptochromes and phytochromes. There is considerable experimental evidence demonstrating the roles of photoreceptors in providing light input to the clock.<h4>Methodology</h4>In order to identify genes regulated by diurnal and circadian rhythms, and to establish possible functional relations between photoreceptors and the circadian clock in tomato, we monitored the temporal transcription pattern in plants entrained to long-day conditions, either by large scale comparative profiling, or using a focused approach over a number of photosensory and clock-related genes by QRT-PCR. In parallel, focused transcription analyses were performed in cry1a- and in CRY2-OX tomato genotypes.<h4>Conclusions</h4>We report a large series of transcript oscillations that shed light on the complex network of interactions among tomato photoreceptors and clock-related genes. Alteration of cryptochrome gene expression induced major changes in the rhythmic oscillations of several other gene transcripts. In particular, over-expression of CRY2 had an impact not only on day/night fluctuations but also on rhythmicity under constant light conditions. Evidence was found for widespread diurnal oscillations of transcripts encoding specific enzyme classes (e.g. carotenoid biosynthesis enzymes) as well as for post-transcriptional diurnal and circadian regulation of the CRY2 transcript.Paolo FacellaLoredana LopezFabrizio CarboneDavid W GalbraithGiovanni GiulianoGaetano PerrottaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 7, p e2798 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Paolo Facella Loredana Lopez Fabrizio Carbone David W Galbraith Giovanni Giuliano Gaetano Perrotta Diurnal and circadian rhythms in the tomato transcriptome and their modulation by cryptochrome photoreceptors.
description	<h4>Background</h4>Circadian clocks are internal molecular time-keeping mechanisms that provide living organisms with the ability to adjust their growth and physiology and to anticipate diurnal environmental changes. Circadian clocks, without exception, respond to light and, in plants, light is the most potent and best characterized entraining stimulus. The capacity of plants to respond to light is achieved through a number of photo-perceptive proteins including cryptochromes and phytochromes. There is considerable experimental evidence demonstrating the roles of photoreceptors in providing light input to the clock.<h4>Methodology</h4>In order to identify genes regulated by diurnal and circadian rhythms, and to establish possible functional relations between photoreceptors and the circadian clock in tomato, we monitored the temporal transcription pattern in plants entrained to long-day conditions, either by large scale comparative profiling, or using a focused approach over a number of photosensory and clock-related genes by QRT-PCR. In parallel, focused transcription analyses were performed in cry1a- and in CRY2-OX tomato genotypes.<h4>Conclusions</h4>We report a large series of transcript oscillations that shed light on the complex network of interactions among tomato photoreceptors and clock-related genes. Alteration of cryptochrome gene expression induced major changes in the rhythmic oscillations of several other gene transcripts. In particular, over-expression of CRY2 had an impact not only on day/night fluctuations but also on rhythmicity under constant light conditions. Evidence was found for widespread diurnal oscillations of transcripts encoding specific enzyme classes (e.g. carotenoid biosynthesis enzymes) as well as for post-transcriptional diurnal and circadian regulation of the CRY2 transcript.
format	article
author	Paolo Facella Loredana Lopez Fabrizio Carbone David W Galbraith Giovanni Giuliano Gaetano Perrotta
author_facet	Paolo Facella Loredana Lopez Fabrizio Carbone David W Galbraith Giovanni Giuliano Gaetano Perrotta
author_sort	Paolo Facella
title	Diurnal and circadian rhythms in the tomato transcriptome and their modulation by cryptochrome photoreceptors.
title_short	Diurnal and circadian rhythms in the tomato transcriptome and their modulation by cryptochrome photoreceptors.
title_full	Diurnal and circadian rhythms in the tomato transcriptome and their modulation by cryptochrome photoreceptors.
title_fullStr	Diurnal and circadian rhythms in the tomato transcriptome and their modulation by cryptochrome photoreceptors.
title_full_unstemmed	Diurnal and circadian rhythms in the tomato transcriptome and their modulation by cryptochrome photoreceptors.
title_sort	diurnal and circadian rhythms in the tomato transcriptome and their modulation by cryptochrome photoreceptors.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/430764ee0e7e468097e4defce9b6c927
work_keys_str_mv	AT paolofacella diurnalandcircadianrhythmsinthetomatotranscriptomeandtheirmodulationbycryptochromephotoreceptors AT loredanalopez diurnalandcircadianrhythmsinthetomatotranscriptomeandtheirmodulationbycryptochromephotoreceptors AT fabriziocarbone diurnalandcircadianrhythmsinthetomatotranscriptomeandtheirmodulationbycryptochromephotoreceptors AT davidwgalbraith diurnalandcircadianrhythmsinthetomatotranscriptomeandtheirmodulationbycryptochromephotoreceptors AT giovannigiuliano diurnalandcircadianrhythmsinthetomatotranscriptomeandtheirmodulationbycryptochromephotoreceptors AT gaetanoperrotta diurnalandcircadianrhythmsinthetomatotranscriptomeandtheirmodulationbycryptochromephotoreceptors
_version_	1718414036406632448

Diurnal and circadian rhythms in the tomato transcriptome and their modulation by cryptochrome photoreceptors.

Ejemplares similares