MicroRNA-378 regulates adipogenic differentiation in bovine intramuscular preadipocytes by targeting CaMKK2

Intramuscular fat, as one of the most important palatability attribute of beef carcase, is the primary determinant of beef quality. The research of adipogenesis mechanism would provide new insight into intramuscular fatty deposition. Here, the role of microRNA-378 was investigated during bovine adip...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dongwei Li, Heng Wang, Yongmin Li, Changqing Qu, Yunhai Zhang, Hongyu Liu, Xiaorong Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	microrna-378 camkk2 adipogenic differentiation bovine intramuscular preadipocytes Diseases of the endocrine glands. Clinical endocrinology RC648-665 Cytology QH573-671 Physiology QP1-981
Acceso en línea:	https://doaj.org/article/4308544633be4f6fbdc2d3afd3828354
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4308544633be4f6fbdc2d3afd3828354
record_format	dspace
spelling	oai:doaj.org-article:4308544633be4f6fbdc2d3afd38283542021-11-04T15:00:45ZMicroRNA-378 regulates adipogenic differentiation in bovine intramuscular preadipocytes by targeting CaMKK22162-39452162-397X10.1080/21623945.2021.1982526https://doaj.org/article/4308544633be4f6fbdc2d3afd38283542021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21623945.2021.1982526https://doaj.org/toc/2162-3945https://doaj.org/toc/2162-397XIntramuscular fat, as one of the most important palatability attribute of beef carcase, is the primary determinant of beef quality. The research of adipogenesis mechanism would provide new insight into intramuscular fatty deposition. Here, the role of microRNA-378 was investigated during bovine adipogenic differentiation. It was revealed that miR-378 expression exists variably in bovine major tissue and organs by RT-qPCR. It was predicted that miR-378 targets CaMKK2, as an AMPKα kinase, by DIANA Tools. For better research, primary preadipocytes with stable transfection for up-/down-regulated expression of miR-378 were constructed by lentiviral vectors with GFP gene. The analyses of qPCR showed that PPARγ and adiponectin mRNA levels increased, but C/EBPβ, pref-1 and CaMKK2 mRNA levels decreased during adipogenic differentiation. When miR-378 was overexpressed, preadipocytes proliferation became slower, there are more cellular lipid droplets, and PPARγ and C/EBPβ mRNA levels were higher, but pref-1, adiponectin and CaMKK2 were lower than control groups. Luciferase assay and western blot analysis validated that miR-378 binds the nucleotide sites of the 3′- untranslated region of CaMKK2, which inhibits the mRNA and protein expression of CaMKK2. These findings suggest that miR-378 promotes adipogenic differentiation in bovine intramuscular preadipocytes by targeting CaMKK2 via AMPK signalling pathway.Dongwei LiHeng WangYongmin LiChangqing QuYunhai ZhangHongyu LiuXiaorong ZhangTaylor & Francis Grouparticlemicrorna-378camkk2adipogenic differentiationbovineintramuscular preadipocytesDiseases of the endocrine glands. Clinical endocrinologyRC648-665CytologyQH573-671PhysiologyQP1-981ENAdipocyte, Vol 10, Iss 1, Pp 483-492 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microrna-378 camkk2 adipogenic differentiation bovine intramuscular preadipocytes Diseases of the endocrine glands. Clinical endocrinology RC648-665 Cytology QH573-671 Physiology QP1-981
spellingShingle	microrna-378 camkk2 adipogenic differentiation bovine intramuscular preadipocytes Diseases of the endocrine glands. Clinical endocrinology RC648-665 Cytology QH573-671 Physiology QP1-981 Dongwei Li Heng Wang Yongmin Li Changqing Qu Yunhai Zhang Hongyu Liu Xiaorong Zhang MicroRNA-378 regulates adipogenic differentiation in bovine intramuscular preadipocytes by targeting CaMKK2
description	Intramuscular fat, as one of the most important palatability attribute of beef carcase, is the primary determinant of beef quality. The research of adipogenesis mechanism would provide new insight into intramuscular fatty deposition. Here, the role of microRNA-378 was investigated during bovine adipogenic differentiation. It was revealed that miR-378 expression exists variably in bovine major tissue and organs by RT-qPCR. It was predicted that miR-378 targets CaMKK2, as an AMPKα kinase, by DIANA Tools. For better research, primary preadipocytes with stable transfection for up-/down-regulated expression of miR-378 were constructed by lentiviral vectors with GFP gene. The analyses of qPCR showed that PPARγ and adiponectin mRNA levels increased, but C/EBPβ, pref-1 and CaMKK2 mRNA levels decreased during adipogenic differentiation. When miR-378 was overexpressed, preadipocytes proliferation became slower, there are more cellular lipid droplets, and PPARγ and C/EBPβ mRNA levels were higher, but pref-1, adiponectin and CaMKK2 were lower than control groups. Luciferase assay and western blot analysis validated that miR-378 binds the nucleotide sites of the 3′- untranslated region of CaMKK2, which inhibits the mRNA and protein expression of CaMKK2. These findings suggest that miR-378 promotes adipogenic differentiation in bovine intramuscular preadipocytes by targeting CaMKK2 via AMPK signalling pathway.
format	article
author	Dongwei Li Heng Wang Yongmin Li Changqing Qu Yunhai Zhang Hongyu Liu Xiaorong Zhang
author_facet	Dongwei Li Heng Wang Yongmin Li Changqing Qu Yunhai Zhang Hongyu Liu Xiaorong Zhang
author_sort	Dongwei Li
title	MicroRNA-378 regulates adipogenic differentiation in bovine intramuscular preadipocytes by targeting CaMKK2
title_short	MicroRNA-378 regulates adipogenic differentiation in bovine intramuscular preadipocytes by targeting CaMKK2
title_full	MicroRNA-378 regulates adipogenic differentiation in bovine intramuscular preadipocytes by targeting CaMKK2
title_fullStr	MicroRNA-378 regulates adipogenic differentiation in bovine intramuscular preadipocytes by targeting CaMKK2
title_full_unstemmed	MicroRNA-378 regulates adipogenic differentiation in bovine intramuscular preadipocytes by targeting CaMKK2
title_sort	microrna-378 regulates adipogenic differentiation in bovine intramuscular preadipocytes by targeting camkk2
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/4308544633be4f6fbdc2d3afd3828354
work_keys_str_mv	AT dongweili microrna378regulatesadipogenicdifferentiationinbovineintramuscularpreadipocytesbytargetingcamkk2 AT hengwang microrna378regulatesadipogenicdifferentiationinbovineintramuscularpreadipocytesbytargetingcamkk2 AT yongminli microrna378regulatesadipogenicdifferentiationinbovineintramuscularpreadipocytesbytargetingcamkk2 AT changqingqu microrna378regulatesadipogenicdifferentiationinbovineintramuscularpreadipocytesbytargetingcamkk2 AT yunhaizhang microrna378regulatesadipogenicdifferentiationinbovineintramuscularpreadipocytesbytargetingcamkk2 AT hongyuliu microrna378regulatesadipogenicdifferentiationinbovineintramuscularpreadipocytesbytargetingcamkk2 AT xiaorongzhang microrna378regulatesadipogenicdifferentiationinbovineintramuscularpreadipocytesbytargetingcamkk2
_version_	1718444765932945408

MicroRNA-378 regulates adipogenic differentiation in bovine intramuscular preadipocytes by targeting CaMKK2

Ejemplares similares