Optimization of manganese bioleaching activity and molecular characterization of indigenous heterotrophic bacteria isolated from the sulfuric area

Abstract. Prasidya DA, Wilopo W, Warmada IW, Retnaningrum E. 2019. Optimization of manganese bioleaching activity and molecular characterization of indigenous heterotrophic bacteria isolated from the sulfuric area. Biodiversitas 20: 1904-1909. The present research evaluated the manganese bioleachin...

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Autores principales:	DENAYA ANDRYA PRASIDYA, WAHYU WILOPO, I WAYAN WARMADA, ENDAH RETNANINGRUM
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	sulfur-oxidizing bacteria (sob), bioleaching, manganese, 16s rrna. Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/430dfe38b4d94ecea4c00ad362a6ad4c
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spelling	oai:doaj.org-article:430dfe38b4d94ecea4c00ad362a6ad4c2021-11-21T21:42:27ZOptimization of manganese bioleaching activity and molecular characterization of indigenous heterotrophic bacteria isolated from the sulfuric area1412-033X2085-472210.13057/biodiv/d200716https://doaj.org/article/430dfe38b4d94ecea4c00ad362a6ad4c2019-06-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2533https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Prasidya DA, Wilopo W, Warmada IW, Retnaningrum E. 2019. Optimization of manganese bioleaching activity and molecular characterization of indigenous heterotrophic bacteria isolated from the sulfuric area. Biodiversitas 20: 1904-1909. The present research evaluated the manganese bioleaching potency of a heterotrophic bacteria KB3B1. This bacterial strain has been isolated from sulfuric area located at Ungaran, Middle of Java, Indonesia using modified 9K medium by adding of several organic nutrients. The manganese bioleaching activities of the strain was analysed by applying of varying glycine concentrations (0, 5, 10, 15 mg mL-1) with pyrolusite pulp densities of 0.02 g cm-3 on a rotary shaker at 180 rpm for 18 days incubation. Several parameters, including the growth of bacteria, pH values, the concentration of soluble manganese and cyanide, were investigated at the interval of 3 days. Molecular characteristics of the strain were further analyzed based on 16S rDNA gene sequences. After 15 days, the maximum yield of manganese 16.6% was achieved under the addition of 10 mg mL-1 glycine. This maximum extract obtained was followed by the maximum bacterial growth, pH, and cyanide product of the strain. Phylogenetic analysis showed that the strain was closely related with Bacillus niacini EP89. Besides, the average frequencies of guanine and cytosine (G+C) of the strain was in same range as that of the reference bacteria in the GenBank and Bergey's Manual Systematics of Bacteria.DENAYA ANDRYA PRASIDYAWAHYU WILOPOI WAYAN WARMADAENDAH RETNANINGRUMMBI & UNS Soloarticlesulfur-oxidizing bacteria (sob), bioleaching, manganese, 16s rrna.Biology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 7 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	sulfur-oxidizing bacteria (sob), bioleaching, manganese, 16s rrna. Biology (General) QH301-705.5
spellingShingle	sulfur-oxidizing bacteria (sob), bioleaching, manganese, 16s rrna. Biology (General) QH301-705.5 DENAYA ANDRYA PRASIDYA WAHYU WILOPO I WAYAN WARMADA ENDAH RETNANINGRUM Optimization of manganese bioleaching activity and molecular characterization of indigenous heterotrophic bacteria isolated from the sulfuric area
description	Abstract. Prasidya DA, Wilopo W, Warmada IW, Retnaningrum E. 2019. Optimization of manganese bioleaching activity and molecular characterization of indigenous heterotrophic bacteria isolated from the sulfuric area. Biodiversitas 20: 1904-1909. The present research evaluated the manganese bioleaching potency of a heterotrophic bacteria KB3B1. This bacterial strain has been isolated from sulfuric area located at Ungaran, Middle of Java, Indonesia using modified 9K medium by adding of several organic nutrients. The manganese bioleaching activities of the strain was analysed by applying of varying glycine concentrations (0, 5, 10, 15 mg mL-1) with pyrolusite pulp densities of 0.02 g cm-3 on a rotary shaker at 180 rpm for 18 days incubation. Several parameters, including the growth of bacteria, pH values, the concentration of soluble manganese and cyanide, were investigated at the interval of 3 days. Molecular characteristics of the strain were further analyzed based on 16S rDNA gene sequences. After 15 days, the maximum yield of manganese 16.6% was achieved under the addition of 10 mg mL-1 glycine. This maximum extract obtained was followed by the maximum bacterial growth, pH, and cyanide product of the strain. Phylogenetic analysis showed that the strain was closely related with Bacillus niacini EP89. Besides, the average frequencies of guanine and cytosine (G+C) of the strain was in same range as that of the reference bacteria in the GenBank and Bergey's Manual Systematics of Bacteria.
format	article
author	DENAYA ANDRYA PRASIDYA WAHYU WILOPO I WAYAN WARMADA ENDAH RETNANINGRUM
author_facet	DENAYA ANDRYA PRASIDYA WAHYU WILOPO I WAYAN WARMADA ENDAH RETNANINGRUM
author_sort	DENAYA ANDRYA PRASIDYA
title	Optimization of manganese bioleaching activity and molecular characterization of indigenous heterotrophic bacteria isolated from the sulfuric area
title_short	Optimization of manganese bioleaching activity and molecular characterization of indigenous heterotrophic bacteria isolated from the sulfuric area
title_full	Optimization of manganese bioleaching activity and molecular characterization of indigenous heterotrophic bacteria isolated from the sulfuric area
title_fullStr	Optimization of manganese bioleaching activity and molecular characterization of indigenous heterotrophic bacteria isolated from the sulfuric area
title_full_unstemmed	Optimization of manganese bioleaching activity and molecular characterization of indigenous heterotrophic bacteria isolated from the sulfuric area
title_sort	optimization of manganese bioleaching activity and molecular characterization of indigenous heterotrophic bacteria isolated from the sulfuric area
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/430dfe38b4d94ecea4c00ad362a6ad4c
work_keys_str_mv	AT denayaandryaprasidya optimizationofmanganesebioleachingactivityandmolecularcharacterizationofindigenousheterotrophicbacteriaisolatedfromthesulfuricarea AT wahyuwilopo optimizationofmanganesebioleachingactivityandmolecularcharacterizationofindigenousheterotrophicbacteriaisolatedfromthesulfuricarea AT iwayanwarmada optimizationofmanganesebioleachingactivityandmolecularcharacterizationofindigenousheterotrophicbacteriaisolatedfromthesulfuricarea AT endahretnaningrum optimizationofmanganesebioleachingactivityandmolecularcharacterizationofindigenousheterotrophicbacteriaisolatedfromthesulfuricarea
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Optimization of manganese bioleaching activity and molecular characterization of indigenous heterotrophic bacteria isolated from the sulfuric area

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