The pPSU Plasmids for Generating DNA Molecular Weight Markers

Abstract Visualizing nucleic acids by gel electrophoresis is one of the most common techniques in molecular biology, and reference molecular weight markers or ladders are commonly used for size estimation. We have created the pPSU1 & pPSU2 pair of molecular weight marker plasmids which produce b...

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Autores principales:	Ryan C. Henrici, Turner J. Pecen, James L. Johnston, Song Tan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/430fdfef37e540a8bb7def120143362c
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spelling	oai:doaj.org-article:430fdfef37e540a8bb7def120143362c2021-12-02T12:32:52ZThe pPSU Plasmids for Generating DNA Molecular Weight Markers10.1038/s41598-017-02693-12045-2322https://doaj.org/article/430fdfef37e540a8bb7def120143362c2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02693-1https://doaj.org/toc/2045-2322Abstract Visualizing nucleic acids by gel electrophoresis is one of the most common techniques in molecular biology, and reference molecular weight markers or ladders are commonly used for size estimation. We have created the pPSU1 & pPSU2 pair of molecular weight marker plasmids which produce both 100 bp and 1 kb DNA ladders when digested with two common restriction enzymes. The 100 bp ladder fragments have been optimized to migrate appropriately on both agarose and native polyacrylamide, unlike many currently available DNA ladders. Sufficient plasmid DNA can be isolated from 100 ml E. coli cultures for the two plasmids to produce 100 bp or 1 kb ladders for 1000 gels. As such, the pPSU1 and pPSU2 plasmids provide reference fragments from 50 to 10000 bp at a fraction of the cost of commercial DNA ladders. The pPSU1 and pPSU2 plasmids are available without licensing restrictions to nonprofit academic users, affording freely available high-quality, low-cost molecular weight standards for molecular biology applications.Ryan C. HenriciTurner J. PecenJames L. JohnstonSong TanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ryan C. Henrici Turner J. Pecen James L. Johnston Song Tan The pPSU Plasmids for Generating DNA Molecular Weight Markers
description	Abstract Visualizing nucleic acids by gel electrophoresis is one of the most common techniques in molecular biology, and reference molecular weight markers or ladders are commonly used for size estimation. We have created the pPSU1 & pPSU2 pair of molecular weight marker plasmids which produce both 100 bp and 1 kb DNA ladders when digested with two common restriction enzymes. The 100 bp ladder fragments have been optimized to migrate appropriately on both agarose and native polyacrylamide, unlike many currently available DNA ladders. Sufficient plasmid DNA can be isolated from 100 ml E. coli cultures for the two plasmids to produce 100 bp or 1 kb ladders for 1000 gels. As such, the pPSU1 and pPSU2 plasmids provide reference fragments from 50 to 10000 bp at a fraction of the cost of commercial DNA ladders. The pPSU1 and pPSU2 plasmids are available without licensing restrictions to nonprofit academic users, affording freely available high-quality, low-cost molecular weight standards for molecular biology applications.
format	article
author	Ryan C. Henrici Turner J. Pecen James L. Johnston Song Tan
author_facet	Ryan C. Henrici Turner J. Pecen James L. Johnston Song Tan
author_sort	Ryan C. Henrici
title	The pPSU Plasmids for Generating DNA Molecular Weight Markers
title_short	The pPSU Plasmids for Generating DNA Molecular Weight Markers
title_full	The pPSU Plasmids for Generating DNA Molecular Weight Markers
title_fullStr	The pPSU Plasmids for Generating DNA Molecular Weight Markers
title_full_unstemmed	The pPSU Plasmids for Generating DNA Molecular Weight Markers
title_sort	ppsu plasmids for generating dna molecular weight markers
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/430fdfef37e540a8bb7def120143362c
work_keys_str_mv	AT ryanchenrici theppsuplasmidsforgeneratingdnamolecularweightmarkers AT turnerjpecen theppsuplasmidsforgeneratingdnamolecularweightmarkers AT jamesljohnston theppsuplasmidsforgeneratingdnamolecularweightmarkers AT songtan theppsuplasmidsforgeneratingdnamolecularweightmarkers AT ryanchenrici ppsuplasmidsforgeneratingdnamolecularweightmarkers AT turnerjpecen ppsuplasmidsforgeneratingdnamolecularweightmarkers AT jamesljohnston ppsuplasmidsforgeneratingdnamolecularweightmarkers AT songtan ppsuplasmidsforgeneratingdnamolecularweightmarkers
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The pPSU Plasmids for Generating DNA Molecular Weight Markers

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