Co-continuous structural effect of size-controlled macro-porous glass membrane on extracellular vesicle collection for the analysis of miRNA

Abstract Recent studies have shown that extracellular vesicles (EVs) can be utilized as appropriate and highly specific biomarkers in liquid biopsy for the diagnosis and prognosis of serious illness. However, there are few methods that can collect and isolate miRNA in EVs simply, quickly and efficie...

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Autores principales:	Hiroshi Yukawa, Shuji Yamazaki, Keita Aoki, Kengo Muto, Naoto Kihara, Kazuhide Sato, Daisuke Onoshima, Takahiro Ochiya, Yasuhito Tanaka, Yoshinobu Baba
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/431686f73b4f471ba3708db7b57033b4
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spelling	oai:doaj.org-article:431686f73b4f471ba3708db7b57033b42021-12-02T13:39:23ZCo-continuous structural effect of size-controlled macro-porous glass membrane on extracellular vesicle collection for the analysis of miRNA10.1038/s41598-021-87986-22045-2322https://doaj.org/article/431686f73b4f471ba3708db7b57033b42021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87986-2https://doaj.org/toc/2045-2322Abstract Recent studies have shown that extracellular vesicles (EVs) can be utilized as appropriate and highly specific biomarkers in liquid biopsy for the diagnosis and prognosis of serious illness. However, there are few methods that can collect and isolate miRNA in EVs simply, quickly and efficiently using general equipment such as a normal centrifuge. In this paper, we developed an advanced glass membrane column (AGC) device incorporating a size-controlled macro-porous glass (MPG) membrane with a co-continuous structure to overcome the limitations of conventional EV collection and miRNA extraction from the EVs. The size of macro-pores in the MPG membrane could be accurately controlled by changing the heating temperature and time on the basis of spinodal decomposition of B2O3, Na2O, and SiO2 in phase separation. The AGC device with an MPG membrane could collect the EVs simply and quickly (< 10 min) from cell culture supernatant, serum and urine. This AGC device could extract miRNA from the EVs captured in the MPG membrane with high efficiency when combined with a miRNA extraction solution. We suggest that the AGC device with an MPG membrane can be useful for the diagnosis and prognosis of serious illness using of EVs in various kinds of body fluids.Hiroshi YukawaShuji YamazakiKeita AokiKengo MutoNaoto KiharaKazuhide SatoDaisuke OnoshimaTakahiro OchiyaYasuhito TanakaYoshinobu BabaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hiroshi Yukawa Shuji Yamazaki Keita Aoki Kengo Muto Naoto Kihara Kazuhide Sato Daisuke Onoshima Takahiro Ochiya Yasuhito Tanaka Yoshinobu Baba Co-continuous structural effect of size-controlled macro-porous glass membrane on extracellular vesicle collection for the analysis of miRNA
description	Abstract Recent studies have shown that extracellular vesicles (EVs) can be utilized as appropriate and highly specific biomarkers in liquid biopsy for the diagnosis and prognosis of serious illness. However, there are few methods that can collect and isolate miRNA in EVs simply, quickly and efficiently using general equipment such as a normal centrifuge. In this paper, we developed an advanced glass membrane column (AGC) device incorporating a size-controlled macro-porous glass (MPG) membrane with a co-continuous structure to overcome the limitations of conventional EV collection and miRNA extraction from the EVs. The size of macro-pores in the MPG membrane could be accurately controlled by changing the heating temperature and time on the basis of spinodal decomposition of B2O3, Na2O, and SiO2 in phase separation. The AGC device with an MPG membrane could collect the EVs simply and quickly (< 10 min) from cell culture supernatant, serum and urine. This AGC device could extract miRNA from the EVs captured in the MPG membrane with high efficiency when combined with a miRNA extraction solution. We suggest that the AGC device with an MPG membrane can be useful for the diagnosis and prognosis of serious illness using of EVs in various kinds of body fluids.
format	article
author	Hiroshi Yukawa Shuji Yamazaki Keita Aoki Kengo Muto Naoto Kihara Kazuhide Sato Daisuke Onoshima Takahiro Ochiya Yasuhito Tanaka Yoshinobu Baba
author_facet	Hiroshi Yukawa Shuji Yamazaki Keita Aoki Kengo Muto Naoto Kihara Kazuhide Sato Daisuke Onoshima Takahiro Ochiya Yasuhito Tanaka Yoshinobu Baba
author_sort	Hiroshi Yukawa
title	Co-continuous structural effect of size-controlled macro-porous glass membrane on extracellular vesicle collection for the analysis of miRNA
title_short	Co-continuous structural effect of size-controlled macro-porous glass membrane on extracellular vesicle collection for the analysis of miRNA
title_full	Co-continuous structural effect of size-controlled macro-porous glass membrane on extracellular vesicle collection for the analysis of miRNA
title_fullStr	Co-continuous structural effect of size-controlled macro-porous glass membrane on extracellular vesicle collection for the analysis of miRNA
title_full_unstemmed	Co-continuous structural effect of size-controlled macro-porous glass membrane on extracellular vesicle collection for the analysis of miRNA
title_sort	co-continuous structural effect of size-controlled macro-porous glass membrane on extracellular vesicle collection for the analysis of mirna
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/431686f73b4f471ba3708db7b57033b4
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Co-continuous structural effect of size-controlled macro-porous glass membrane on extracellular vesicle collection for the analysis of miRNA

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