Potassium as a pluripotency-associated element identified through inorganic element profiling in human pluripotent stem cells

Abstract Despite their well-known function in maintaining normal cell physiology, how inorganic elements are relevant to cellular pluripotency and differentiation in human pluripotent stem cells (hPSCs) has yet to be systematically explored. Using total reflection X-ray fluorescence (TXRF) spectrome...

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Autores principales:	Victor J. T. Lin, Ashwini Zolekar, Yi Shi, Bhuvaneswari Koneru, Slobodan Dimitrijevich, Anthony J. Di Pasqua, Yu-Chieh Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/43197e935d0f4140911b3661046ff9bf
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spelling	oai:doaj.org-article:43197e935d0f4140911b3661046ff9bf2021-12-02T11:52:19ZPotassium as a pluripotency-associated element identified through inorganic element profiling in human pluripotent stem cells10.1038/s41598-017-05117-22045-2322https://doaj.org/article/43197e935d0f4140911b3661046ff9bf2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05117-2https://doaj.org/toc/2045-2322Abstract Despite their well-known function in maintaining normal cell physiology, how inorganic elements are relevant to cellular pluripotency and differentiation in human pluripotent stem cells (hPSCs) has yet to be systematically explored. Using total reflection X-ray fluorescence (TXRF) spectrometry and inductively coupled plasma mass spectrometry (ICP-MS), we analyzed the inorganic components of human cells with isogenic backgrounds in distinct states of cellular pluripotency. The elemental profiles revealed that the potassium content of human cells significantly differs when their cellular pluripotency changes. Pharmacological treatment that alters cell membrane permeability to potassium affected the maintenance and establishment of cellular pluripotency via multiple mechanisms in bona fide hPSCs and reprogrammed cells. Collectively, we report that potassium is a pluripotency-associated inorganic element in human cells and provide novel insights into the manipulation of cellular pluripotency in hPSCs by regulating intracellular potassium.Victor J. T. LinAshwini ZolekarYi ShiBhuvaneswari KoneruSlobodan DimitrijevichAnthony J. Di PasquaYu-Chieh WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Victor J. T. Lin Ashwini Zolekar Yi Shi Bhuvaneswari Koneru Slobodan Dimitrijevich Anthony J. Di Pasqua Yu-Chieh Wang Potassium as a pluripotency-associated element identified through inorganic element profiling in human pluripotent stem cells
description	Abstract Despite their well-known function in maintaining normal cell physiology, how inorganic elements are relevant to cellular pluripotency and differentiation in human pluripotent stem cells (hPSCs) has yet to be systematically explored. Using total reflection X-ray fluorescence (TXRF) spectrometry and inductively coupled plasma mass spectrometry (ICP-MS), we analyzed the inorganic components of human cells with isogenic backgrounds in distinct states of cellular pluripotency. The elemental profiles revealed that the potassium content of human cells significantly differs when their cellular pluripotency changes. Pharmacological treatment that alters cell membrane permeability to potassium affected the maintenance and establishment of cellular pluripotency via multiple mechanisms in bona fide hPSCs and reprogrammed cells. Collectively, we report that potassium is a pluripotency-associated inorganic element in human cells and provide novel insights into the manipulation of cellular pluripotency in hPSCs by regulating intracellular potassium.
format	article
author	Victor J. T. Lin Ashwini Zolekar Yi Shi Bhuvaneswari Koneru Slobodan Dimitrijevich Anthony J. Di Pasqua Yu-Chieh Wang
author_facet	Victor J. T. Lin Ashwini Zolekar Yi Shi Bhuvaneswari Koneru Slobodan Dimitrijevich Anthony J. Di Pasqua Yu-Chieh Wang
author_sort	Victor J. T. Lin
title	Potassium as a pluripotency-associated element identified through inorganic element profiling in human pluripotent stem cells
title_short	Potassium as a pluripotency-associated element identified through inorganic element profiling in human pluripotent stem cells
title_full	Potassium as a pluripotency-associated element identified through inorganic element profiling in human pluripotent stem cells
title_fullStr	Potassium as a pluripotency-associated element identified through inorganic element profiling in human pluripotent stem cells
title_full_unstemmed	Potassium as a pluripotency-associated element identified through inorganic element profiling in human pluripotent stem cells
title_sort	potassium as a pluripotency-associated element identified through inorganic element profiling in human pluripotent stem cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/43197e935d0f4140911b3661046ff9bf
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Potassium as a pluripotency-associated element identified through inorganic element profiling in human pluripotent stem cells

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