Antibody isotype diversity against SARS-CoV-2 is associated with differential serum neutralization capacities
Abstract Understanding antibody responses to SARS-CoV-2 is indispensable for the development of containment measures to overcome the current COVID-19 pandemic. Recent studies showed that serum from convalescent patients can display variable neutralization capacities. Still, it remains unclear whethe...
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Nature Portfolio
2021
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oai:doaj.org-article:431b486be8244906b88b8f8c1a505e1f2021-12-02T13:20:04ZAntibody isotype diversity against SARS-CoV-2 is associated with differential serum neutralization capacities10.1038/s41598-021-84913-32045-2322https://doaj.org/article/431b486be8244906b88b8f8c1a505e1f2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84913-3https://doaj.org/toc/2045-2322Abstract Understanding antibody responses to SARS-CoV-2 is indispensable for the development of containment measures to overcome the current COVID-19 pandemic. Recent studies showed that serum from convalescent patients can display variable neutralization capacities. Still, it remains unclear whether there are specific signatures that can be used to predict neutralization. Here, we performed a detailed analysis of sera from a cohort of 101 recovered healthcare workers and we addressed their SARS-CoV-2 antibody response by ELISA against SARS-CoV-2 Spike receptor binding domain and nucleoprotein. Both ELISA methods detected sustained levels of serum IgG against both antigens. Yet, the majority of individuals from our cohort generated antibodies with low neutralization capacity and only 6% showed high neutralizing titers against both authentic SARS-CoV-2 virus and the Spike pseudotyped virus. Interestingly, higher neutralizing sera correlate with detection of -IgG, IgM and IgA antibodies against both antigens, while individuals with positive IgG alone showed poor neutralization response. These results suggest that having a broader repertoire of antibodies may contribute to more potent SARS-CoV-2 neutralization. Altogether, our work provides a cross sectional snapshot of the SARS-CoV-2 neutralizing antibody response in recovered healthcare workers and provides preliminary evidence that possessing multiple antibody isotypes can play an important role in predicting SARS-CoV-2 neutralization.Maria G. NovalMaria E. KaczmarekAkiko KoideBruno A. Rodriguez-RodriguezPing LouieTakuya TadaTakamitsu HattoriTatyana PanchenkoLarizbeth A. RomeroKai Wen TengAndrew BazleyMaren de VriesMarie I. SamanovicJeffrey N. WeiserIoannis AifantisJoan CangiarellaMark J. MulliganLudovic DesvignesMeike DittmannNathaniel R. LandauMaria Aguero-RosenfeldShohei KoideKenneth A. StaplefordNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
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Medicine R Science Q |
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Medicine R Science Q Maria G. Noval Maria E. Kaczmarek Akiko Koide Bruno A. Rodriguez-Rodriguez Ping Louie Takuya Tada Takamitsu Hattori Tatyana Panchenko Larizbeth A. Romero Kai Wen Teng Andrew Bazley Maren de Vries Marie I. Samanovic Jeffrey N. Weiser Ioannis Aifantis Joan Cangiarella Mark J. Mulligan Ludovic Desvignes Meike Dittmann Nathaniel R. Landau Maria Aguero-Rosenfeld Shohei Koide Kenneth A. Stapleford Antibody isotype diversity against SARS-CoV-2 is associated with differential serum neutralization capacities |
| description |
Abstract Understanding antibody responses to SARS-CoV-2 is indispensable for the development of containment measures to overcome the current COVID-19 pandemic. Recent studies showed that serum from convalescent patients can display variable neutralization capacities. Still, it remains unclear whether there are specific signatures that can be used to predict neutralization. Here, we performed a detailed analysis of sera from a cohort of 101 recovered healthcare workers and we addressed their SARS-CoV-2 antibody response by ELISA against SARS-CoV-2 Spike receptor binding domain and nucleoprotein. Both ELISA methods detected sustained levels of serum IgG against both antigens. Yet, the majority of individuals from our cohort generated antibodies with low neutralization capacity and only 6% showed high neutralizing titers against both authentic SARS-CoV-2 virus and the Spike pseudotyped virus. Interestingly, higher neutralizing sera correlate with detection of -IgG, IgM and IgA antibodies against both antigens, while individuals with positive IgG alone showed poor neutralization response. These results suggest that having a broader repertoire of antibodies may contribute to more potent SARS-CoV-2 neutralization. Altogether, our work provides a cross sectional snapshot of the SARS-CoV-2 neutralizing antibody response in recovered healthcare workers and provides preliminary evidence that possessing multiple antibody isotypes can play an important role in predicting SARS-CoV-2 neutralization. |
| format |
article |
| author |
Maria G. Noval Maria E. Kaczmarek Akiko Koide Bruno A. Rodriguez-Rodriguez Ping Louie Takuya Tada Takamitsu Hattori Tatyana Panchenko Larizbeth A. Romero Kai Wen Teng Andrew Bazley Maren de Vries Marie I. Samanovic Jeffrey N. Weiser Ioannis Aifantis Joan Cangiarella Mark J. Mulligan Ludovic Desvignes Meike Dittmann Nathaniel R. Landau Maria Aguero-Rosenfeld Shohei Koide Kenneth A. Stapleford |
| author_facet |
Maria G. Noval Maria E. Kaczmarek Akiko Koide Bruno A. Rodriguez-Rodriguez Ping Louie Takuya Tada Takamitsu Hattori Tatyana Panchenko Larizbeth A. Romero Kai Wen Teng Andrew Bazley Maren de Vries Marie I. Samanovic Jeffrey N. Weiser Ioannis Aifantis Joan Cangiarella Mark J. Mulligan Ludovic Desvignes Meike Dittmann Nathaniel R. Landau Maria Aguero-Rosenfeld Shohei Koide Kenneth A. Stapleford |
| author_sort |
Maria G. Noval |
| title |
Antibody isotype diversity against SARS-CoV-2 is associated with differential serum neutralization capacities |
| title_short |
Antibody isotype diversity against SARS-CoV-2 is associated with differential serum neutralization capacities |
| title_full |
Antibody isotype diversity against SARS-CoV-2 is associated with differential serum neutralization capacities |
| title_fullStr |
Antibody isotype diversity against SARS-CoV-2 is associated with differential serum neutralization capacities |
| title_full_unstemmed |
Antibody isotype diversity against SARS-CoV-2 is associated with differential serum neutralization capacities |
| title_sort |
antibody isotype diversity against sars-cov-2 is associated with differential serum neutralization capacities |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/431b486be8244906b88b8f8c1a505e1f |
| work_keys_str_mv |
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1718393205423079424 |