Osteoinductive Moldable and Curable Bone Substitutes Based on Collagen, BMP-2 and Highly Porous Polylactide Granules, or a Mix of HAP/β-TCP

In dentistry, maxillofacial surgery, traumatology, and orthopedics, there is a need to use osteoplastic materials that have not only osteoinductive and osteoconductive properties but are also convenient for use. In the study, compositions based on collagen hydrogel were developed. Polylactide granul...

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Autores principales:	Andrey Vyacheslavovich Vasilyev, Valeriya Sergeevna Kuznetsova, Tatyana Borisovna Bukharova, Egor Olegovich Osidak, Timofei Evgenevich Grigoriev, Yuriy Dmitrievich Zagoskin, Irina Alekseevna Nedorubova, Sergey Petrovich Domogatsky, Igor Ivanovich Babichenko, Oksana Aleksandrovna Zorina, Sergey Ivanovich Kutsev, Sergei Nicolaevich Chvalun, Anatoly Alekseevich Kulakov, Fedor Fedorovich Losev, Dmitry Vadimovich Goldshtein
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	collagen fibronectin hydrogel polylactide HAP/β-TCP BMP-2 Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/431d569b506d4293928eecde5dea7515
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spelling	oai:doaj.org-article:431d569b506d4293928eecde5dea75152021-11-25T18:49:00ZOsteoinductive Moldable and Curable Bone Substitutes Based on Collagen, BMP-2 and Highly Porous Polylactide Granules, or a Mix of HAP/β-TCP10.3390/polym132239742073-4360https://doaj.org/article/431d569b506d4293928eecde5dea75152021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/22/3974https://doaj.org/toc/2073-4360In dentistry, maxillofacial surgery, traumatology, and orthopedics, there is a need to use osteoplastic materials that have not only osteoinductive and osteoconductive properties but are also convenient for use. In the study, compositions based on collagen hydrogel were developed. Polylactide granules (PLA) or a traditional bone graft, a mixture of hydroxyapatite and β-tricalcium phosphate (HAP/β-TCP), were used for gel filling to improve mechanical osteoconductive properties of compositions. The mechanical tests showed that collagen hydrogels filled with 12 wt% highly porous PLA granules (elastic modulus 373 ± 55 kPa) or 35 wt% HAP/β-TCP granules (elastic modulus 451 ± 32 kPa) had optimal manipulative properties. All composite components were cytocompatible. The cell’s viability was above 90%, and the components’ structure facilitated the cell’s surface adhesion. The bone morphogenetic protein-2 (BMP-2) provided osteoinductive composition properties. It was impregnated directly into the collagen hydrogel with the addition of fibronectin or inside porous PLA granules. The implantation of a collagen hydrogel with BMP-2 and PLA granules into a critical-size calvarial defect in rats led to the formation of the most significant volume of bone tissue: 61 ± 15%. It was almost 2.5 times more than in the groups where a collagen-fibronectin hydrogel with a mixture of HAP/β-TCP (25 ± 7%) or a fibronectin-free composition with porous PLA granules impregnated with BMP-2 (23 ± 8%) were used. Subcutaneous implantation of the compositions also showed their high biocompatibility and osteogenic potential in the absence of a bone environment. Thus, the collagen-fibronectin hydrogel with BMP-2 and PLA granules has optimal biocompatibility, osteogenic, and manipulative properties.Andrey Vyacheslavovich VasilyevValeriya Sergeevna KuznetsovaTatyana Borisovna BukharovaEgor Olegovich OsidakTimofei Evgenevich GrigorievYuriy Dmitrievich ZagoskinIrina Alekseevna NedorubovaSergey Petrovich DomogatskyIgor Ivanovich BabichenkoOksana Aleksandrovna ZorinaSergey Ivanovich KutsevSergei Nicolaevich ChvalunAnatoly Alekseevich KulakovFedor Fedorovich LosevDmitry Vadimovich GoldshteinMDPI AGarticlecollagenfibronectinhydrogelpolylactideHAP/β-TCPBMP-2Organic chemistryQD241-441ENPolymers, Vol 13, Iss 3974, p 3974 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	collagen fibronectin hydrogel polylactide HAP/β-TCP BMP-2 Organic chemistry QD241-441
spellingShingle	collagen fibronectin hydrogel polylactide HAP/β-TCP BMP-2 Organic chemistry QD241-441 Andrey Vyacheslavovich Vasilyev Valeriya Sergeevna Kuznetsova Tatyana Borisovna Bukharova Egor Olegovich Osidak Timofei Evgenevich Grigoriev Yuriy Dmitrievich Zagoskin Irina Alekseevna Nedorubova Sergey Petrovich Domogatsky Igor Ivanovich Babichenko Oksana Aleksandrovna Zorina Sergey Ivanovich Kutsev Sergei Nicolaevich Chvalun Anatoly Alekseevich Kulakov Fedor Fedorovich Losev Dmitry Vadimovich Goldshtein Osteoinductive Moldable and Curable Bone Substitutes Based on Collagen, BMP-2 and Highly Porous Polylactide Granules, or a Mix of HAP/β-TCP
description	In dentistry, maxillofacial surgery, traumatology, and orthopedics, there is a need to use osteoplastic materials that have not only osteoinductive and osteoconductive properties but are also convenient for use. In the study, compositions based on collagen hydrogel were developed. Polylactide granules (PLA) or a traditional bone graft, a mixture of hydroxyapatite and β-tricalcium phosphate (HAP/β-TCP), were used for gel filling to improve mechanical osteoconductive properties of compositions. The mechanical tests showed that collagen hydrogels filled with 12 wt% highly porous PLA granules (elastic modulus 373 ± 55 kPa) or 35 wt% HAP/β-TCP granules (elastic modulus 451 ± 32 kPa) had optimal manipulative properties. All composite components were cytocompatible. The cell’s viability was above 90%, and the components’ structure facilitated the cell’s surface adhesion. The bone morphogenetic protein-2 (BMP-2) provided osteoinductive composition properties. It was impregnated directly into the collagen hydrogel with the addition of fibronectin or inside porous PLA granules. The implantation of a collagen hydrogel with BMP-2 and PLA granules into a critical-size calvarial defect in rats led to the formation of the most significant volume of bone tissue: 61 ± 15%. It was almost 2.5 times more than in the groups where a collagen-fibronectin hydrogel with a mixture of HAP/β-TCP (25 ± 7%) or a fibronectin-free composition with porous PLA granules impregnated with BMP-2 (23 ± 8%) were used. Subcutaneous implantation of the compositions also showed their high biocompatibility and osteogenic potential in the absence of a bone environment. Thus, the collagen-fibronectin hydrogel with BMP-2 and PLA granules has optimal biocompatibility, osteogenic, and manipulative properties.
format	article
author	Andrey Vyacheslavovich Vasilyev Valeriya Sergeevna Kuznetsova Tatyana Borisovna Bukharova Egor Olegovich Osidak Timofei Evgenevich Grigoriev Yuriy Dmitrievich Zagoskin Irina Alekseevna Nedorubova Sergey Petrovich Domogatsky Igor Ivanovich Babichenko Oksana Aleksandrovna Zorina Sergey Ivanovich Kutsev Sergei Nicolaevich Chvalun Anatoly Alekseevich Kulakov Fedor Fedorovich Losev Dmitry Vadimovich Goldshtein
author_facet	Andrey Vyacheslavovich Vasilyev Valeriya Sergeevna Kuznetsova Tatyana Borisovna Bukharova Egor Olegovich Osidak Timofei Evgenevich Grigoriev Yuriy Dmitrievich Zagoskin Irina Alekseevna Nedorubova Sergey Petrovich Domogatsky Igor Ivanovich Babichenko Oksana Aleksandrovna Zorina Sergey Ivanovich Kutsev Sergei Nicolaevich Chvalun Anatoly Alekseevich Kulakov Fedor Fedorovich Losev Dmitry Vadimovich Goldshtein
author_sort	Andrey Vyacheslavovich Vasilyev
title	Osteoinductive Moldable and Curable Bone Substitutes Based on Collagen, BMP-2 and Highly Porous Polylactide Granules, or a Mix of HAP/β-TCP
title_short	Osteoinductive Moldable and Curable Bone Substitutes Based on Collagen, BMP-2 and Highly Porous Polylactide Granules, or a Mix of HAP/β-TCP
title_full	Osteoinductive Moldable and Curable Bone Substitutes Based on Collagen, BMP-2 and Highly Porous Polylactide Granules, or a Mix of HAP/β-TCP
title_fullStr	Osteoinductive Moldable and Curable Bone Substitutes Based on Collagen, BMP-2 and Highly Porous Polylactide Granules, or a Mix of HAP/β-TCP
title_full_unstemmed	Osteoinductive Moldable and Curable Bone Substitutes Based on Collagen, BMP-2 and Highly Porous Polylactide Granules, or a Mix of HAP/β-TCP
title_sort	osteoinductive moldable and curable bone substitutes based on collagen, bmp-2 and highly porous polylactide granules, or a mix of hap/β-tcp
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/431d569b506d4293928eecde5dea7515
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Osteoinductive Moldable and Curable Bone Substitutes Based on Collagen, BMP-2 and Highly Porous Polylactide Granules, or a Mix of HAP/β-TCP

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